A5068308281- Cancer-related molecular mechanisms research
- Colorectal Cancer Treatments and Studies
- Genetic factors in colorectal cancer
- RNA modifications and cancer
- MicroRNA in disease regulation
- Cervical Cancer and HPV Research
- RNA Research and Splicing
- Cancer Genomics and Diagnostics
- Liver Disease Diagnosis and Treatment
- Cellular transport and secretion
- Inflammatory Bowel Disease
- Liver Diseases and Immunity
- Lymphoma Diagnosis and Treatment
- Antibiotic Resistance in Bacteria
- Bladder and Urothelial Cancer Treatments
- Pituitary Gland Disorders and Treatments
- S100 Proteins and Annexins
- Diet and metabolism studies
- Immune cells in cancer
- RNA and protein synthesis mechanisms
- Cancer Immunotherapy and Biomarkers
- Gene expression and cancer classification
- Cancer-related Molecular Pathways
- IL-33, ST2, and ILC Pathways
- Epigenetics and DNA Methylation
University of Warsaw
2019-2025
The Maria Sklodowska-Curie National Research Institute of Oncology
2015-2024
Genomics (United Kingdom)
2024
Centrum Onkologii
2017-2020
Institute of Biochemistry and Biophysics, Polish Academy of Sciences
2016
Postgraduate School of Molecular Medicine
2009-2011
Gdańsk Medical University
2009
Background Clinical progression of colorectal cancers (CRC) may occur in parallel with distinctive signaling alterations. We designed multidirectional analyses integrating microarray-based data biostatistics and bioinformatics to elucidate the metabolic alterations underlying CRC development adenoma-carcinoma sequence. Methodology/Principal Findings Studies were performed on normal mucosa, adenoma, carcinoma samples obtained during surgery or colonoscopy. Collections cryostat sections...
Microplastic particles from the air are inhaled and accumulate in lungs, potentially causing immunological reactions airway tissue injury. This study aimed to evaluate biological effects of polyamide fibres on nasal epithelium co-cultivated with macrophages control, asthma, COPD groups. Nasal epithelial cells alone or co-culture monocyte-derived were exposed for 48 h. We identified 8 differentially expressed genes (DEGs) controls, 309 DEGs asthma (including ANKRD36C, BCL2L15, FCGBP, IL-19),...
Inhibition of oncogenic transcriptional programs is a promising therapeutic strategy. A substituted tricyclic benzimidazole, SEL120-34A, novel inhibitor Cyclin-dependent kinase 8 (CDK8), which regulates transcription by associating with the Mediator complex. X-ray crystallography has shown SEL120-34A to be type I forming halogen bonds protein's hinge region and hydrophobic complementarities within its front pocket. inhibits phosphorylation STAT1 S727 STAT5 S726 in cancer cells vitro....
We analysed critically the potential usefulness of RNA- and DNA-based biomarkers in supporting conventional histological diagnostic tests for prostate carcinoma (PCa) detection. Microarray profiling gene expression DNA methylation was performed on 16 benign prostatic hyperplasia (BPH) 32 cancerous non-cancerous samples extracted by radical prostatectomy. The predictive value selected validated qPCR-based methods using tissue from 58 prostates and, separately, 227 core biopsies. HOXC6, AMACR...
Irritable bowel syndrome (IBS) is a chronic functional disorder and its development may be linked, directly indirectly, to intestinal dysbiosis. Here we investigated the interactions between IBS symptoms gut microbiome, including relation rifaximin (1200 mg daily; 11.2 g per treatment). We recruited 72 patients, 31 with IBS-D (diarrhea), 11 IBS-C (constipation), 30 IBS-M (mixed constipation diarrhea) healthy controls (HCs). Of them, 68%, 64%, 53% patients IBS-D, IBS-C, IBS-M, respectively,...
Background: Clinical progression of colorectal cancers (CRC) may occur in parallel with distinctive signaling alterations.We designed multidirectional analyses integrating microarray-based data biostatistics and bioinformatics to elucidate the metabolic alterations underlying CRC development adenoma-carcinoma sequence.Methodology/Principal Findings: Studies were performed on normal mucosa, adenoma, carcinoma samples obtained during surgery or colonoscopy.Collections cryostat sections...
Vulvar squamous cell carcinoma (VSCC) constitutes over 90% of vulvar cancer. Its pathogenesis can follow two different pathways; high risk human papillomavirus (hrHPV)-dependent and HPV-independent. Due to the rarity VSCC, molecular mechanisms underlying VSCC development remain largely unknown. The study aimed identify pathogenic mutations implicated in pathways development.Using next generation sequencing, 81 tumors, 52 hrHPV(+) 29 hrHPV(-), were screened for hotspot 50 genes covered by Ion...
Asthma and chronic obstructive pulmonary disease (COPD) are heterogeneous diseases with different inflammatory phenotypes. Various mediators play a role in these diseases. The aim of this study was to analyze the neutrophilic eosinophilic airway systemic inflammation as phenotypic characterization patients asthma COPD. Twenty-four 33 COPD were enrolled study. All mild-to-moderate stage disease, none them treated inhaled corticosteroids. Concentrations IL-6, neutrophil elastase (NE), matrix...
Most inflammatory bowel diseases (IBDs) are classic complex disorders represented by common alleles. Here we aimed to define the genetic architecture of pediatric and adult-onset IBDs for Polish population. A total 1495 patients were recruited, including 761 with Crohn's disease (CD; 424 pediatric), 734 ulcerative colitis (UC; 390 934 healthy controls. Allelotyping employed a pooled-DNA genome-wide association study (GWAS) was validated individual genotyping. Whole exome sequencing (WES)...
Article13 January 2020Open Access Source DataTransparent process Synthetic lethality between VPS4A and VPS4B triggers an inflammatory response in colorectal cancer Ewelina Szymańska Corresponding Author [email protected] orcid.org/0000-0001-7994-0764 Laboratory of Cell Biology, International Institute Molecular Warsaw, Poland Search for more papers by this author Paulina Nowak Krzysztof Kolmus Magdalena Cybulska Department Genetics, Maria Skłodowska-Curie Institute-Oncology Centre, Goryca...
About 50% of non-small cell lung cancer (NSCLC) patients develop distant metastases following pulmonary resection. Currently, there are no reliable factors allowing for individual selection high-risk adjuvant systemic therapies. We assessed by quantitative reverse transcription PCR microRNA (miRNA) expression in 273 stage I–IIIA NSCLC samples. Expression 677 miRNAs was evaluated fresh-frozen tumour samples the training cohort 50 squamous carcinoma (SCC) who underwent curative surgery. Of...
Excess double-stranded RNA (dsRNA) is present in the cytoplasm of human cells, usually following viral infections. Recognition dsRNAs activates innate immune pathways, leading to cellular inflammation and inhibition cell growth. Here, we show that an effective dsRNA response may occur without onset inflammation. Pro-inflammatory [RLR (retinoic acid–inducible gene I–like receptor)–dependent pathway] growth inhibitory mechanisms [oligoadenylate synthetase (OAS)/ribonuclease L (RNase L)–...
The heterogeneous nuclear ribonucleoprotein K (hnRNPK) is a nucleic acid-binding protein that acts as docking platform integrating signal transduction pathways to acid-related processes. Given hnRNPK could be involved in other steps compose gene expression the definition of its genome-wide occupancy important better understand role transcription and co-transcriptional Here, we used chromatin immunoprecipitation followed by deep sequencing (ChIP-Seq) analyze hnRNPK-DNA interaction colon...
Genome-wide mechanisms that coordinate expression of subsets functionally related genes are largely unknown. Recent studies show receptor tyrosine kinases and components signal transduction cascades including the extracellular signal-regulated protein kinase (ERK), once thought to act predominantly in vicinity plasma membrane cytoplasm, can be recruited chromatin encompassing transcribed genes. distribution these transducers their relationship transcribing RNA polymerase II (Pol2) could...
Approximately 90% of colorectal cancer (CRC) deaths are caused by tumors ability to migrate into the adjacent tissues and metastase distant organs. More than 40 genes have been causally linked development CRC but no mutations associated with metastasis yet. To identify molecular basis we performed whole-exome genome-scale transcriptome sequencing 7 liver metastases along their matched primary tumours normal tissue. Multiple, spatially separated fragments were analyzed in each case. Uniformly...
Abstract Primary biliary cholangitis (PBC), primary sclerosing (PSC), and inflammatory bowel diseases (IBDs), including Crohn’s disease (CD) ulcerative colitis (UC), are heterogeneous chronic autoimmune that may share underlying pathogenic mechanisms. Herein, we compared simultaneously analyzed blood transcriptomes from patients with PBC, PSC, IBD. Microarray-based measurements were conducted using RNA isolated whole samples 90, 45, 95 93 CD, UC, respectively, 47 healthy controls. Expression...
Current knowledge on the biology of squamous cell vulvar carcinoma (VSCC) is limited. We aimed to identify protein markers VSCC tumors that would permit stratify patients by progression risk. Early-stage from who progressed (progVSCC) and those were disease-free (d-fVSCC) during follow-up, along with normal tissues examined mass spectrometry-based proteomics. Differentially expressed proteins (DEPs) then verified in solid blood samples premalignant lesions. In progVSCC vs. d-fVSCC tumors,...
Abstract Background A causal link between microbiota composition (dysbiosis) and oncogenesis has been demonstrated for several types of cancer. Neutrophils play a role in both immune protection against bacterial threats carcinogenesis. This study aimed to characterise intratumoral bacteria vulvar squamous cell carcinoma (VSCC) their putative effect on neutrophil recruitment cancer progression. Methods Clinical material was obtained from 89 patients with VSCC. Next-generation sequencing (NGS)...
Comprehensive whole-genome sequencing was performed on two multi-drug-resistant
The red microalga Cyanidioschyzon merolae inhabits extreme environments with high temperatures (40–56 °C), acidity (pH 0.05–4), and concentrations of heavy metals that are lethal to most forms life. However, information is scarce on the precise adaptation mechanisms this extremophile such hostile conditions. Gaining knowledge important for understanding evolution microorganisms in early stages life Earth characterized by environments. Through an analysis re-programming global transcriptome...
Altered epigenetic mechanisms, similar to gene mutations, contribute the pathogenesis and molecular heterogeneity of neoplasms, including colorectal cancer (CRC). Enhancer zeste 2 (EZH2) is a histone methyltransferase, which involved in silencing aberrantly expressed CRC. Therefore, identification genes regulated by EZH2 CRC important improve current understanding its role epigenetics. The present study used chromatin immunoprecipitation (ChIP) followed deep sequencing assess genome-wide...
Uterine sarcomas and mixed epithelial–mesenchymal uterine tumors are a heterogeneous group of rare for which there very few diagnostic markers available. As aberrant microRNA (miRNA) expression patterns represent putative cancer markers, we aimed to identify miRNA profiles the major sarcoma subtypes uterus. Eighty-eight miRNAs were assessed by quantitative RT-PCR in cancerous non-cancerous tissue samples collected from 29 patients with endometrial sarcoma, leiomyosarcoma, tumors. Tumor...