A5010768340- Microbial Community Ecology and Physiology
- Bacteriophages and microbial interactions
- Genomics and Phylogenetic Studies
- Polar Research and Ecology
- Bacterial Genetics and Biotechnology
- Antibiotic Resistance in Bacteria
- Protist diversity and phylogeny
- Anaerobic Digestion and Biogas Production
- RNA and protein synthesis mechanisms
- Probiotics and Fermented Foods
- Helminth infection and control
- Milk Quality and Mastitis in Dairy Cows
- Agriculture, Plant Science, Crop Management
- Agriculture and Biological Studies
- Plant Virus Research Studies
- Veterinary Oncology Research
- Biofuel production and bioconversion
- Parasitic Infections and Diagnostics
- Pharmaceutical and Antibiotic Environmental Impacts
- Machine Learning in Bioinformatics
- Plant Pathogens and Resistance
- Enterobacteriaceae and Cronobacter Research
- Microbial infections and disease research
- biodegradable polymer synthesis and properties
- Escherichia coli research studies
Institute of Biochemistry and Biophysics, Polish Academy of Sciences
2016-2025
Polish Academy of Sciences
2011-2020
University of Florida
2017
University of Warsaw
2017
University of Newcastle Australia
2017
Museum and Institute of Zoology
2017
Witold Stefański Institute of Parasitology
2007-2016
Potato (Solanum tuberosum L.) is the world's most important non-grain food crop and central to global security. It clonally propagated, highly heterozygous, autotetraploid, suffers acute inbreeding depression. Here we use a homozygous doubled-monoploid potato clone sequence assemble 86% of 844-megabase genome. We predict 39,031 protein-coding genes present evidence for at least two genome duplication events indicative palaeopolyploid origin. As first an asterid, reveals 2,642 specific this...
Polaromonas is one of the most abundant genera found on glacier surfaces, yet its ecology remains poorly described. Investigations made to date point towards a uniform distribution phylotypes across globe. We compared 43 isolates obtained from surfaces Arctic and Antarctic glaciers address this issue. 16S rRNA gene sequences, intergenic transcribed spacers (ITS) metabolic fingerprinting showed great differences between hemispheres but also neighboring glaciers. Phylogenetic distance...
During the acetogenic step of anaerobic digestion, products acidogenesis are oxidized to substrates for methanogenesis: hydrogen, carbon dioxide and acetate. Acetogenesis methanogenesis highly interconnected processes due syntrophic associations between bacteria hydrogenotrophic methanogens, allowing whole process become thermodynamically favorable. The aim this study is determine influence dominant acidic on metabolic pathways methane formation find a core microbiome substrate-specific...
Achlorophyllous unicellular microalgae of the genus Prototheca (Trebouxiophyceae, Chlorophyta) are only known plants that cause infections in both humans and animals, collectively referred to as protothecosis. Human protothecosis, most commonly manifested cutaneous, articular, disseminated disease, is primarily caused by Protothecawickerhamii, followed Protothecazopfii and, sporadically, Protothecacutis Protothecamiyajii In veterinary medicine, however, P. zopfii a major pathogen responsible...
Cold-active bacteria of the genus Polaromonas (class Betaproteobacteria) are important components glacial microbiomes. In this study, extrachromosomal replicons 26 psychrotolerant strains, isolated from Arctic and Antarctic glaciers, were identified, sequenced, characterized. The plasmidome these strains consists 13 replicons, ranging in size 3,378 to 101,077 bp. silico sequence analyses identified conserved backbones plasmids, composed genes required for plasmid replication, stable...
The complex cell wall structure of algae often precludes efficient extraction their genetic material. purpose this study was to design a next-generation sequencing-suitable DNA isolation method for unicellular, achlorophyllous, yeast-like microalgae the genus Prototheca, only known plant pathogens both humans and animals. effectiveness newly proposed scheme compared with five other, previously described methods, commonly used from plants and/or yeasts, available either as...
Phage vB_SauP_phiAGO1.3 (phiAGO1.3) is a polyvalent Staphylococcus lytic podovirus with 17.6-kb genome (Gozdek et al., 2018). It can infect most of the aureus human isolates dominant clonal complexes. We show that major factor contributing to wide host range phiAGO1.3 lack or sparcity target sites for certain restriction-modification systems types I and II in its genome. requires adsorption β-O-GlcNAcylated cell wall teichoic acid, which also essential expression methicillin resistance....
Glaciers have recently been recognized as ecosystems comprised of several distinct habitats: a sunlit and oxygenated glacial surface, ice, dark, mostly anoxic bed. Surface meltwaters annually flood the subglacial sediments by means drainage channels. Glacial surfaces host aquatic microhabitats called cryoconite holes, regarded "hot spots" microbial abundance activity, largely contributing to meltwaters' bacterial diversity. This study presents an investigation hole anaerobes discusses their...
The pace at which multidrug-resistant strains emerge has been alarming. P. stuartii is an infrequent but relevant drug-resistant nosocomial pathogen causing local to systemic life-threatening infections. We propose alternative approach fight this bacterium based on the properties of phage tailspikes with depolymerase activity that degrade surface bacterial polymers, making bacteria susceptible immune system. Unlike antibiotics, have narrow and specific substrate spectra, by acting as...
Atopic dermatitis (AD) is a common inflammatory skin disease, for which dysbiosis of the mycobiome considered triggering factor. The aim this study was to explore AD patients and healthy volunteers (HV). included 50 as many HV. Culture-based species identification involved battery conventional phenotypic tests PCR sequencing internal transcribed spacer (ITS) 1 2 regions within rDNA cluster. Culture-independent, metataxonomic performed with ITS1 target region. overall culture-positive rate...
Propionic acid (PA) is an important organic with applications in food preservation, feed additives, and bio-based chemical production. While industrial PA mostly derived from petrochemical processes, sustainable microbial alternatives are gaining attention. In this study, we explored a co-fermentation strategy using lactic bacteria (LAB) complementary metabolic capabilities to enhance biosynthesis via the 1,2-propanediol (PDO) pathway. Genome-based screening identified division between...
Sourdough fermentation is a traditional process that used to improve bread quality. A spontaneous sourdough ecosystem consists of mixture flour and water fermented by endogenous lactic acid bacteria (LAB) yeasts. The aim this study was identify bacterial diversity during backslopping sourdoughs prepared from wheat, spelt, or rye wholemeal flour. Culture-dependent analyses showed the number LAB (109 CFU/ml) higher three orders magnitude than yeasts (106 CFU/ml), irrespective type. These...
Comprehensive whole-genome sequencing was performed on two multi-drug-resistant
Various Lactobacillus paracasei strains are found in diverse environments, including dairy and plant materials the intestinal tract of humans animals, also used food industry or as probiotics. In this study, we have isolated a new strain L. subsp. IBB3423 from samples raw cow milk collected citizen science project. showed some desired probiotic features such high adhesion capacity ability to metabolize inulin. Its complete genome sequence comprising chromosome 3,183,386 bp two plasmids 5986...
Colobanthus quitensis (Kunth) Bartl. and Deschampsia antarctica Desv. are the only Magnoliophyta to naturally colonize Antarctic region. The reason for their sole presence in Antarctica is still debated as there no definitive consensus on how two unrelated flowering plants managed establish breeding populations this part of world. In study, we have explored compared rhizosphere root-endosphere dwelling microbial community C. D. specimens sampled maritime from sites displaying contrasting...
Introduction Bacteria of genus Pectobacterium , encompassing economically significant pathogens affecting various plants, includes the species P. betavasculorum initially associated with beetroot infection. However, its host range is much broader. It causes diseases sunflower, potato, tomato, carrots, sweet radish, squash, cucumber, and chrysanthemum. To explain this phenomenon, a comprehensive pathogenomic phenomic characterisation was performed. Methods Genomes strains isolated from...
Abstract The cereal leaf beetle (CLB, Oulema melanopus ) is one of the major pests. effect insecticides belonging to different chemical classes, with mechanisms action and active substances' concentrations on CLB bacterial microbiome, was investigated. Targeted metagenomic analysis V3–V4 regions 16S ribosomal gene used determine composition microbiome. Each caused a decrease in abundance bacteria genus Pantoea , an increase Stenotrophomonas Acinetobacter compared untreated insects. After...
Pseudomonas aeruginosa is a cause of nosocomial infections, especially in patients with cystic fibrosis and burn wounds. PAO1 strain its derivatives are widely used to study the biology this bacterium, however recent studies demonstrated differences genomes phenotypes from different laboratories.Here we report genome sequence P. PAO1161 laboratory strain, leu-, RifR, restriction-modification defective derivative, described as host IncP-8 plasmid FP2, conferring resistance mercury. Comparison...
Emerging important Acinetobacter strains commonly accommodate a plethora of mobile elements including plasmids different size. Plasmids, apart from encoding modules enabling their self-replication and/or transmission, can carry diverse number genes, allowing the host cell to survive in an environment that would otherwise be lethal or restrictive for growth. The present study characterizes plasmidome generated arsenic-resistant strain named ZS207, classified as lwoffii. Sequencing effort...
Poa annua (annual bluegrass) is one of the most ubiquitous grass species in world. In isolated regions maritime Antarctica, it has become an invasive organism threatening native tundra communities. this study, we have explored and compared rhizosphere root-endosphere dwelling microbial community P. specimens Antarctic Central European origin terms bacterial phylogenetic diversity metabolic activity with a geochemical soil background. Our results show that rhizospheric was unique for each...
In the majority of bacterial species, tripartite ParAB-parS system, composed an ATPase (ParA), a DNA-binding protein (ParB), and its target parS sequence(s), assists in chromosome partitioning. ParB forms large nucleoprotein complexes at parS(s), located vicinity origin chromosomal replication (oriC), which after are subsequently positioned by ParA cell poles. Remarkably, participate not only segregation but through interactions with various cellular partners they also involved other...