- Plant Molecular Biology Research
- Plant biochemistry and biosynthesis
- Plant Pathogens and Fungal Diseases
- Photosynthetic Processes and Mechanisms
- Light effects on plants
- Plant Gene Expression Analysis
- Horticultural and Viticultural Research
- Plant Pathogens and Resistance
- Berry genetics and cultivation research
- Plant Physiology and Cultivation Studies
- Greenhouse Technology and Climate Control
- Postharvest Quality and Shelf Life Management
- Plant Virus Research Studies
- Edible Oils Quality and Analysis
- Insect-Plant Interactions and Control
- Chromosomal and Genetic Variations
- Pancreatitis Pathology and Treatment
- Mitochondrial Function and Pathology
- Phytochemicals and Antioxidant Activities
- Plant-Microbe Interactions and Immunity
- Plant tissue culture and regeneration
- Plant Disease Resistance and Genetics
- Genomics and Phylogenetic Studies
- Cystic Fibrosis Research Advances
- Plant Reproductive Biology
Inserm
2024
Université de Strasbourg
2024
Centre National de la Recherche Scientifique
2002-2024
Institut de génétique et de biologie moléculaire et cellulaire
2024
National Agency for New Technologies, Energy and Sustainable Economic Development
2010-2019
University of Calabria
1996-2019
Research Centre for Olive Growing and Olive Oil Industry
2019
ENEA Trisaia Research Centre
2007-2017
Erasmus Hospital
2017
ENEA Casaccia Research Centre
2016
Potato (Solanum tuberosum L.) is the world's most important non-grain food crop and central to global security. It clonally propagated, highly heterozygous, autotetraploid, suffers acute inbreeding depression. Here we use a homozygous doubled-monoploid potato clone sequence assemble 86% of 844-megabase genome. We predict 39,031 protein-coding genes present evidence for at least two genome duplication events indicative palaeopolyploid origin. As first an asterid, reveals 2,642 specific this...
Coffee, tea, and chocolate converge Caffeine has evolved multiple times among plant species, but no one knows whether these events involved similar genes. Denoeud et al. sequenced the Coffea canephora (coffee) genome identified a conserved gene order (see Perspective by Zamir). Although this species underwent fewer duplications than related relevant caffeine genes experienced tandem that expanded their numbers within species. Scientists have seen independent expansions in distantly of tea...
Cryptochromes are blue light photoreceptors found in plants, bacteria, and animals. In Arabidopsis, cryptochrome 2 (cry2) is involved primarily the control of flowering time photomorphogenesis under low-fluence light. No data on function cry2 available apart from Arabidopsis (Arabidopsis thaliana). Expression tomato (Solanum lycopersicum) CRY2 gene was altered through a combination transgenic overexpression virus-induced silencing. Tomato overexpressors show phenotypes similar to but...
Abstract Background Despite its primary economic importance, genomic information on olive tree is still lacking. 454 pyrosequencing was used to enrich the very few sequence data currently available for Olea europaea species and identify genes involved in expression of fruit quality traits. Results Fruits Coratina , a widely cultivated variety characterized by high phenolic content, Tendellone an oleuropein-lacking natural variant, were as starting material monitoring transcriptome. Four...
The influence of internal (genetic and developmental) external (environmental) factors on levels flavonoid gene transcripts, enzyme activity metabolites was studied in fruit six cultivated strawberry (Fragaria x ananassa Duch.) genotypes grown at two Italian locations. Gene expression showed development- genotype-associated patterns, revealing coordination. Analysis clarified the regulation mechanism hydroxylation status B-ring major pools pointed out examples genotype-specific...
Olive (Olea europaea L.) fruits contain numerous secondary metabolites, primarily phenolics, terpenes and sterols, some of which are particularly interesting for their nutraceutical properties. This study will attempt to provide further insight into the profile olive phenolic compounds during fruit development identify major genetic determinants metabolism.The concentration compounds, such as oleuropein, demethyloleuropein, 3-4 DHPEA-EDA, ligstroside, tyrosol, hydroxytyrosol, verbascoside...
The olive tree lacks dormancy and is low temperature sensitive, with differences in cold tolerance oil quality among genotypes. produced the drupe, unsaturated fatty acids contribute to its quality. aim of present research was investigate relationship development, response, expression acid desaturase (FAD) genes, composition drupes belonging genotypes differing leaf tolerance, but producing good (i.e. non-hardy Moraiolo, semi-hardy Frantoio, hardy Canino). In all genotypes, sensitivity,...
Starting from the categorical definitions of "depressive disorders", we proceeded to list individual forms provided by DSM-V, with a particular focus on historical, clinical, neurobiological and therapeutic profiles,
The identification and characterization of transcripts involved in flower organ development, plant reproduction metabolism represent key steps phenotypic physiological pathways, may generate high-quality transcript variants useful for the development functional markers. This study was aimed at obtaining an extensive olive transcripts, by providing sound information on candidate MADS-box genes related to ABC model putative genetic molecular determinants ovary abortion pollen-pistil...
Cryptochromes are flavin-containing blue/UVA light photoreceptors that regulate various plant light-induced physiological processes. In Arabidopsis (Arabidopsis thaliana), cryptochromes mediate de-etiolation, photoperiodic control of flowering, entrainment the circadian clock, cotyledon opening and expansion, anthocyanin accumulation, root growth. tomato (Solanum lycopersicum), encoded by a multigene family, comprising CRY1a, CRY1b, CRY2, CRY3 We have previously reported phenotypes cry1a...
Microscopic investigations were conducted into the interaction of Colletotrichum acutatum on white and red strawberry ( Fragaria ×ananassa ) fruit surfaces. The results showed that, whilst early events similar in both fruits, after 24 h fungal colonization dramatically varied: fruits C. became quiescent as melanized appressoria, but it displayed subcuticular necrotrophic invasion. A microarray analysis strawberries with was performed, order to reveal differences gene expression possibly...
Wheat is one of the world's most important crops and characterized by a large polyploid genome. One way to reduce genome complexity isolate single chromosomes using flow cytometry. Low coverage DNA sequencing can provide snapshot individual chromosomes, allowing fast characterization their main features comparison with other genomes. We used massively parallel 454 pyrosequencing obtain 2x wheat chromosome 5A. The resulting sequence assembly was identify TEs, genes miRNAs, as well infer...
Summary Several novel allelic groups of tomato ( Solanum lycopersicum L.) mutants with impaired photomorphogenesis have been identified after γ‐ray mutagenesis phyA phyB1 double‐mutant seed. Recessive in one group are characterized by retarded hook opening, increased hypocotyl elongation and reduced chlorophyll content under white light (WL). These showed a specific impairment response to blue (BL) resulting from lesions the gene encoding BL receptor cryptochrome 1 (cry1). Phytochrome A cry1...
Background Circadian clocks are internal molecular time-keeping mechanisms that provide living organisms with the ability to adjust their growth and physiology anticipate diurnal environmental changes. clocks, without exception, respond light and, in plants, is most potent best characterized entraining stimulus. The capacity of plants achieved through a number photo-perceptive proteins including cryptochromes phytochromes. There considerable experimental evidence demonstrating roles...
Cucumber mosaic virus (CMV), a member of the Cucumovirus genus, is causal agent several plant diseases in wide range host species, causing important economic losses agriculture. Because lack natural resistance genes most crops, different genetic engineering strategies have been adopted to obtain virus-resistant plants. In previous study, we described transgenic tomato plants expressing single-chain variable fragment antibody (scFv G4) that are specifically protected from CMV infection. this...
In order to sense and respond the fluctuating light conditions, higher plants possess several families of photoreceptors, such as phytochromes (PHYs), cryptochromes (CRYs) phototropins. CRYs are responsible for photomorphogenesis play a role in circadian, developmental adaptive growth regulation plants. tomato (Solanum lycopersicum), CRY2 controls vegetative development, flowering time, fruit antioxidant content well diurnal transcription other photoreceptor genes. We applied large-scale...
Background Widespread in the Mediterranean basin, Olea europaea trees are gaining worldwide popularity for nutritional and cancer-protective properties of oil, mechanically extracted from ripe fruits. Fruit development is a physiological process with remarkable impact on modulation biosynthesis compounds affecting quality drupes as well final composition olive oil. Proteomics offers possibility to dig deeper into major changes during fruit development, including important phase ripening,...
Chronic airway infection is a hallmark feature of cystic fibrosis (CF) disease. In the present study, sputum samples from CF patients were collected and characterized by 16S rRNA gene-targeted approach, to assess how lung microbiota composition changes following severe decline in function. particular, we compared two groups with CF, i.e. substantial their function (SD) stable (S). The showed different bacterial composition, SD reporting more heterogeneous community than S ones. Pseudomonas...
The olive fruit fly Bactrocera oleae (Diptera: Tephritidae) is the most devastating pest of cultivated (Olea europaea L.). Intraspecific variation in plant resistance to B. has been described only at phenotypic level. In this work, we used a transcriptomic approach study molecular response two cultivars with contrasting level susceptibility. Using next-generation pyrosequencing, first generated catalogue more than 80,000 sequences expressed drupes from approximately 700k reads. assembled...