A5106128344- Plant Pathogens and Resistance
- Microbial infections and disease research
- Chromosomal and Genetic Variations
- T-cell and B-cell Immunology
- Plant Disease Resistance and Genetics
- Toxin Mechanisms and Immunotoxins
- Vector-borne infectious diseases
- Potato Plant Research
- Biochemical and Structural Characterization
- Aquaculture disease management and microbiota
- Plant Pathogenic Bacteria Studies
- Plant Virus Research Studies
- Phytoplasmas and Hemiptera pathogens
- Escherichia coli research studies
- Mycotoxins in Agriculture and Food
- Powdery Mildew Fungal Diseases
- Plant nutrient uptake and metabolism
- Connective tissue disorders research
- Plant Micronutrient Interactions and Effects
- Immune Cell Function and Interaction
- Insect Pest Control Strategies
- Elasticity and Material Modeling
- Immune Response and Inflammation
- Vector-Borne Animal Diseases
- Sugarcane Cultivation and Processing
Universidade de São Paulo
2022
Instituto de Botânica
2021
Universidad Peruana Cayetano Heredia
2011-2018
Centre de Coopération Internationale en Recherche Agronomique pour le Développement
1996-2006
Institut Pasteur de la Guadeloupe
1999-2004
Centre Hospitalier Universitaire Pointe-à-Pitre
1996-1998
University of Hawaii System
1997
Utrecht University
1995
Potato (Solanum tuberosum L.) is the world's most important non-grain food crop and central to global security. It clonally propagated, highly heterozygous, autotetraploid, suffers acute inbreeding depression. Here we use a homozygous doubled-monoploid potato clone sequence assemble 86% of 844-megabase genome. We predict 39,031 protein-coding genes present evidence for at least two genome duplication events indicative palaeopolyploid origin. As first an asterid, reveals 2,642 specific this...
The genome of potato, a major global food crop, was recently sequenced. work presented here details the integration potato reference (DM) with new sequence-tagged site marker-based linkage map and other physical genetic maps closely related species tomato. Primary anchoring DM assembly accomplished by use diploid segregating population, which genotyped several types molecular markers to construct ~936 cM comprising 2469 marker loci. In silico approaches used from genotype RH89-039-16 (RH)...
Abstract Background Sugarcane is capable to store large amounts of sucrose in the culm at maturity hence it became a major source for food and renewable energy industries. Sucrose, main disaccharide produced by photosynthesis, mainly stored vacuole cells non-photosynthetic tissues. Two pathways are known release free plant cells, one de novo synthesis dependent on phosphate synthase (SPS) phosphatase (S6PP) while other regulatory (SuSy) activity. The molecular understanding genes that give...
Currently available serological tests for cowdriosis (Cowdria ruminantium infection) in domestic ruminants are hampered by their low specificities because of cross-reactivity with Ehrlichia spp. The use recombinant major antigenic protein (MAP1) C. serodiagnosis was investigated. Overlapping fragments the MAP1 were expressed Escherichia coli and reacted sera from sheep infected either or ovina. Two immunogenic regions on protein, designated MAP1-A MAP1-B, identified. reactive antisera, E....
A competitive enzyme-linked immunosorbent assay (cELISA) was developed to detect immunoglobulin G antibodies the major 32-kDa protein of Cowdria ruminantium. total 1,804 serum samples collected from cattle on 19 islands in eastern Caribbean Basin were tested by this cELISA. 133 10 (Antigua, Dominica, Grenada, Guadeloupe, Martinique, Montserrat, St. Kitts, Lucia, Martin, and Vincent) found be positive. The presence C. ruminantium these confirmed immunofluorescence Western blotting...
SUMMARY Ehrlichia ruminantium is an obligate intracellular bacterium that causes heartwater in ruminants and for which T‐cell‐mediated immunity believed to play important role protection. To better characterize protective cellular immunity, E. ‐specific IFN‐γ IL‐4 recall responses major T‐cell subsets were analysed by flow cytometry during immunization of goats with a killed vaccine following virulent challenge. The elicited both CD8 + CD4 produce cytoplasmic the absence IL‐4, thus...
Les auteurs rapportent les résultats de suivis séro-épidémiologiques réalisés dans la zone sahélienne, des Niayes et en nord-guinéenne respectivement sur zébus, bovins Diakoré taurins Ndama. Des veaux âgés 0 à 3 mois ou 6 ont fait l'objet prélèvements sang tous trimestres ceci durant environ 20 mois. Au test d'immunofluorescence, 70 % 1 donnent positifs vis-à-vis l'antigène Cowdria ruminantium, tandis que prévalence est 92 entre Au-delà du 2e trimestre, on obtient 100 positifs. En...
La helada es el estrés abiótico caracterizado por la disminución de temperatura del aire debajo cero grados centígrados, uno los problemas mayor incidencia e impacto en agricultura andina. Por otro lado, estudios morfológicos y fisiológicos señalan a las papas nativas como fuentes tolerancia genética al helada. Sin embargo, bases moleculares mecanismos papa, aún son desconocidas. tecnología secuenciamiento ARN (RNA-seq) una excelente herramienta que permite identificar cambios perfiles...
A bstract : Cellular responses induced in two Creole goats by vaccination with killed Cowdria ruminantium ( ) were confirmed IFN‐γ production and interleukin‐2 receptor (IL‐2R) expression. Both CD4 + CD8 but not WC1 T cells showed a substantial increase cell surface expression of IL‐2R molecules response to whole lysate. (Welgevonden strain) proteins fractionated using continuous‐flow electrophoresis tested for their ability induce PBMC collected three weeks after the first inoculation one...
The exposure of plants to drought conditions inhibits shoot growth, increases production toxic reactive oxygen species (ROS), and negatively affects photosynthesis carbohydrate metabolism. Drought susceptibility in potato impacts all stages the crop, from emergence tuber initiation. Although there have been various studies global changes gene expression profiles during potatoes, few based on native species. Because their high genetic diversity, potatoes Andean regions Peru, Ecuador Bolivia,...