A5026203422- Probiotics and Fermented Foods
- Bacterial Genetics and Biotechnology
- Microbial Metabolites in Food Biotechnology
- Bacteriophages and microbial interactions
- Genomics and Phylogenetic Studies
- RNA and protein synthesis mechanisms
- Nutrition and Health Studies
- Viral gastroenteritis research and epidemiology
- Gut microbiota and health
- Antibiotic Resistance in Bacteria
- Microbial Metabolic Engineering and Bioproduction
- Polyamine Metabolism and Applications
- Animal Nutrition and Physiology
- Enzyme Structure and Function
- Meat and Animal Product Quality
- Biochemical and Structural Characterization
- Antimicrobial Peptides and Activities
- Oral microbiology and periodontitis research
- Protein Hydrolysis and Bioactive Peptides
- Enzyme Production and Characterization
- Microbial infections and disease research
- Antimicrobial Resistance in Staphylococcus
- Steroid Chemistry and Biochemistry
- Digestive system and related health
- Enterobacteriaceae and Cronobacter Research
Institute of Biochemistry and Biophysics, Polish Academy of Sciences
2012-2023
Polish Academy of Sciences
2000-2015
University of Warsaw
2015
Warsaw University of Life Sciences
2014
Mossakowski Medical Research Institute, Polish Academy of Sciences
2011
Institut National de la Recherche Agronomique
1998
Département Génétique Animale
1992-1998
Medical University of Warsaw
1978-1985
ABSTRACT Oscypek is a traditional Polish scalded-smoked cheese, with protected-designation-of-origin (PDO) status, manufactured from raw sheep's milk without starter cultures in the Tatra Mountains region of Poland. This study was undertaken order to gain insight into microbiota that develops and evolves during manufacture ripening stages Oscypek. To this end, we made use both culturing culture-independent methods PCR followed by denaturing gradient gel electrophoresis (PCR-DGGE)...
Here we report the nucleotide sequence of pCTX-M3, a highly conjugative plasmid that is responsible for extensive spread gene coding CTX-M-3 extended-spectrum beta-lactamase in clinical populations family Enterobacteriaceae Poland. The belongs to IncL/M incompatibility group, 89,468 bp size, and carries 103 putative genes. Besides bla(CTX-M-3), it also bears bla(TEM-1), aacC2, armA genes, as well integronic aadA2, dfrA12, sul1, which altogether confer resistance majority beta-lactams...
A trial was conducted to evaluate the effect of in ovo injection prebiotic and synbiotics on growth performance, meat quality traits (cholesterol content, intramuscular collagen properties, fiber measurements), presence histopathological changes pectoral muscle (PS) broiler chickens. On d 12 incubation, 480 eggs were randomly divided into 5 experimental groups treated with different bioactives, injected: C, control physiological saline; T1 1.9 mg raffinose family oligosaccharides; T2 T3...
The Lactococcus lactis subsp. strains isolated from dairy products are auxotrophs for branched-chain amino acids (leucine, isoleucine, and valine), while most nondairy media prototrophs. We have cloned sequenced the leu genes one auxotroph, IL1403. sequence is 99% homologous to that of prototroph NCDO2118, which was determined previously. Two nonsense mutations two small deletions were found in auxotroph sequence, might explain acid auxotrophy. Nevertheless, appear be transcribed regulated...
Prebiotics and probiotics applied alone or together (synbiotics) can influence the intestinal microbiota modulate immune response. We analyzed impact of in ovo administration synbiotics on system development Ross (broiler) Green-legged Partridgelike (GP, dual-purpose fowl) chickens. For delivery 12th day eggs incubation, two strains lactic acid bacteria (LAB) were used, i.e. Lactococcus lactis subsp. IBB SL1 (S1) cremoris SC1 (S2), combined with raffinose family oligosaccharides (RFO)...
In eukaryotes, RNA species originating from pervasive transcription are regulators of various cellular processes, the expression individual genes to control development and oncogenesis. prokaryotes, function its output on cell physiology is still unknown. Most bacteria possess termination factor Rho, which represses pervasive, mostly antisense, transcription. Here, we investigate biological significance Rho-controlled in Gram-positive model bacterium Bacillus subtilis. Rho inactivation...
Titanium dioxide (TiO2) is commonly used as a food additive (E171 in the EU) for its whitening and opacifying properties. However, risk of intestinal barrier disruption, including dysbiosis gut microbiota, increasingly suspected because presence nano-sized fraction this additive. We hypothesized that food-grade E171 Aeroxyde P25 (identical to NM-105 OECD reference nanomaterial European Union Joint Research Centre) interact with both commensal bacteria transient food-borne under...
Lactic acid bacteria (LAB) are Gram-positive which considered for use as adjuvant therapeutics in management of various disease ailments, including obesity, irritable bowel syndrome, lactose intolerance and cancer. To investigate the possible Lactococcus lactis strains from our collection treatment gastrointestinal cancer, we tested them ability to arrest proliferation human colorectal adenocarcinoma cells (Caco-2). Results BrdU assay showed that anti-proliferative activity L. is...
Summary All 47 non-nisin producing strains of Streptococcus lactis and 12/13 Str. subsp. diacetylactis examined produced bacteriocins, for which the term lactostrepcins is suggested. Seven cremoris no bacteriocins active against 3 lactic streptococci used as indicators. The were divided into groups: I, those only one streptomycin resistant mutant 60 indicator strain; II, all strains; III, not employed. sensitive to various proteolytic enzymes phospholipase D, but retained full or partial...
Abstract Campylobacter jejuni / coli infections are the leading cause of bacterial diarrheal illnesses in humans. Many epidemiological studies indicate that improperly prepared meat from chickens carry a high load their intestinal tracts is key source human infections. LAB , mainly members Lactococcus and Lactobacillus genera, increasingly have been tested as vehicles for delivery heterologous or viral antigens to animal mucosal immune systems. Thus, objective this study was isolate,...
A fragment of the Lactococcus lactis chromosome containing an open reading frame 265 codons, denoted bglR, has been characterized. The polypeptide encoded by bglR shares 36 to 30% sequence identity with a family regulatory proteins including ArbG from Erwinia chrysanthemi, BglG Escherichia coli, and SacT SacY Bacillus subtilis. These are involved in positive control utilization different sugars transcription antitermination. For some these it demonstrated that antitermination is exerted...
The extrachromosomal gene pool plays a significant role both in evolution and the environmental adaptation of bacteria. L. lactis subsp. IL594 strain contains seven plasmids, named pIL1 to pIL7, is parental plasmid-free IL1403, which one best characterized lactococcal strains LAB. Complete nucleotide sequences (6,382 bp), pIL2 (8,277 pIL3 (19,244 pIL4 (48,979), pIL5 (23,395), pIL6 (28,435 bp) pIL7 (28,546) were established deposited generally accessible database (GeneBank). Nine highly...
The Lactococcus lactis chromosomal region containing the seven structural genes required for tryptophan biosynthesis was characterized by cloning and sequencing. All of trp were identified homology their products with known Trp proteins from other organisms. identification confirmed five ability to complement mutations in Escherichia coli. are present order trpEGDCFBA span a 7,968-bp segment. Each gene is preceded putative ribosome binding site complementary 3' end L. 16S rRNA. Three pairs...
ABSTRACT The Lactococcus lactis trpEGDCFBA operon is preceded by a noncoding leader region. Transcriptional studies of the trp revealed three transcripts with respective sizes 8 kb (encompassing entire operon), 290 bases, and 160 bases (corresponding to parts region). These most likely result from initiation at unique P promoter, transcription termination either T1 (upstream operon) or T2 (downstream and/or processing. Three parameters were shown differentially affect amount these...
We present here the results of an exploration bacteriophage content dairy wheys collected from milk plants localized in various regions Poland. Thirty-three whey samples 17 were analyzed and found to contain phages active against L. lactis strains. High phage titer all suggested phage-induced lysis be main cause fermentation failures. In total, over 220 isolated examined for their restriction patterns, genome sizes, genetic groups DNA homology, host ranges. Based on digestions identified...
Lactobacillus rhamnosus LOCK900 fulfills the criteria required for probiotic strains. In this study, we report a whole-genome sequence of isolate and compare it with other L. complete genome sequences already published.
Sourdough fermentation is a traditional process that used to improve bread quality. A spontaneous sourdough ecosystem consists of mixture flour and water fermented by endogenous lactic acid bacteria (LAB) yeasts. The aim this study was identify bacterial diversity during backslopping sourdoughs prepared from wheat, spelt, or rye wholemeal flour. Culture-dependent analyses showed the number LAB (109 CFU/ml) higher three orders magnitude than yeasts (106 CFU/ml), irrespective type. These...
Escherichia coli isolates recovered from patients during a clonal outbreak in Warsaw, Poland, hospital 1997 produced different levels of an extended-spectrum beta-lactamase (ESBL) the SHV type. The hyperproduction correlated with multiplication ESBL gene copies within plasmid. Here, we present complete nucleotide sequence plasmid p1658/97 carried by outbreak. is 125,491 bp and shows mosaic structure which all modules constituting core are homologous to those found plasmids F R100 separated...
Tetracycline-resistant Lactococcus lactis strains originally isolated from Polish raw milk were analyzed for the ability to transfer their antibiotic resistance genes in vitro, using filter mating experiments, and vivo, germfree rats. Four of six L. isolates able tetracycline determinants vitro Bu2-60, at frequencies ranging 10(-5) 10(-7) transconjugants per recipient. Three these four could also Enterococcus faecalis JH2-2, whereas no Bacillus subtilis YBE01, Pseudomonas putida KT2442,...