Synbiotics are synergistic combinations of prebiotics and probiotics. In chickens, synbiotics can be delivered in ovo to expedite colonization the gut by beneficial bacteria. We therefore aimed design vitro validate them broiler chickens upon delivery. The probiotic components were Lactobacillus salivarius plantarum. Their growth was assessed MRS medium supplemented with different prebiotics. Based on results (hatchability curve), two designed: S1 -Lactobacillus galactooligosaccarides (GOS)...

Intestinal mucosa is the interface between microbial content of gut and host's milieu. The goal this study was to modulate chicken intestinal microflora by in ovo stimulation with galactooligosaccharides (GOS) prebiotic demonstrate molecular responses host. animal trial performed on meat-type chickens (Ross 308). GOS delivered injection into air cell day 12 egg incubation. Analysis communities mucosal gene expression at slaughter (day 42 post-hatching). Chyme (for DNA isolation) RNA from...

The effects of heat stress (HS) caused by high temperatures continue to be a global concern in poultry production. Poultry birds are homoeothermic, however, modern-day chickens highly susceptible HS due their inefficiency dissipating from body the lack sweat glands. During HS, load is higher than chickens' ability regulate it. This can disturb normal physiological functioning, affect metabolism and cause behavioural changes, respiratory alkalosis immune dysregulation birds. These adverse gut...

To determine immunomodulatory effects of synbiotics administered in ovo on immune-related gene expression adult chickens.30 Green-legged Partridgelike chickens.On incubation day 12, eggs were injected with 3 (Lactococcus lactis subsp IBB SL1 raffinose family oligosaccharides [RFOs; S1], Lactococcus cremoris SC1 RFOs [S2], and Lactobacillus acidophilus Streptococcus faecium lactose [S3]). Control prebiotic or saline (0.9% NaCl) solution. Gene 6 cytokines (interleukin [IL]-4, IL-6, IL-12p40,...

Probiotics and phytobiotics have demonstrated effective improvement of gut health in broiler chickens when individually administered in-ovo. However, their combined use in-ovo, has not been studied to date. We coined the term "prophybiotic" (probiotic + phytobiotic) for such a combination. The current study therefore, aimed elucidate effects selected probiotic phytobiotic on production parameters, as opposed probiotics alone. ROSS 308 hatching eggs were injected with either Leuconostoc...

In the European Union, legislation concerning animal housing is becoming stricter because of welfare concerns. Feather pecking (FP) in large group systems a major problem. It has been suggested that corticosterone (CORT) response to manual restraint as measure for stress associated with FP behavior. The aim current study was identify QTL involved behavior and laying hens. An F2 population 630 hens established from cross between two commercial lines differing their propensity feather peck....

Prebiotics and probiotics applied alone or together (synbiotics) can influence the intestinal microbiota modulate immune response. We analyzed impact of in ovo administration synbiotics on system development Ross (broiler) Green-legged Partridgelike (GP, dual-purpose fowl) chickens. For delivery 12th day eggs incubation, two strains lactic acid bacteria (LAB) were used, i.e. Lactococcus lactis subsp. IBB SL1 (S1) cremoris SC1 (S2), combined with raffinose family oligosaccharides (RFO)...

In ovo delivery of prebiotics and synbiotics in chickens allows for the development intestinal microflora prior to hatching, which boosts their robustness. The goal this study was determine transcriptomic profile spleen (S), cecal tonsils (CT), large intestine (LI) adult injected with ovo. On day 12 embryo development, incubating eggs were prebiotics: inulin alone (P1) or combination Lactococcus lactis subsp. IBB2955 (S1), galactooligosaccharides (GOS) (P2) cremoris IBB477 (S2); control...

The current research was conducted to compare growth, carcass traits, pH, intramuscular collagen (IMC) properties, and genetic bases of IMC carcasses (breast-muscle weight) different lines generations adult males females Japanese quail (Coturnix japonica). Forty-four quails (generation F0), 22 Pharaoh (F-33) meat-type Standard (S-22) laying-type females, were crossed produce the F1 hybrids generation. F2 generation created by mating one male with female, full siblings. birds, randomly chosen...

Background: A previous study showed that prebiotics and synbiotics administered in ovo into the egg air cell on 12th day of incubation enhance growth development chickens.However, influence this procedure efficiency innate immune system broiler chickens is unclear.Therefore, aim was to evaluate whether early (on embryo development) administration selected (inulin -Pre1 Bi 2 tos -Pre2) + Lactococcus lactis subsp.lactis IBB SL1 -Syn1 L. subsp.cremoris SC1 -Syn2) influences system.Results:...

OBJECTIVE To evaluate the effect of in ovo administration inulin and Lactococcus lactis on immune-related gene expression broiler chickens. ANIMALS 45 Ross broilers. PROCEDURES On day 12 embryonic development, 360 eggs were equally allocated among 3 treatment groups injected with 0.2 mL a solution that contained 1.76 mg (prebiotic group) or enriched 1,000 CFUs L subsp 2955 (synbiotic group), they saline (0.9% NaCl) (control). At 1, 14, 35 days after hatching, 5 male birds from each group...

The effect of the in ovo application selected prebiotics and synbiotics on humoral immune response against T-dependent (SRBC) T-independent (dextran) antigens delayed-type hypersensitivity (DTH) to phytohemagglutinin was studied. On 12th day incubation, 800 eggs (Ross 308) were divided into five groups injected egg air chamber with prebiotic inulin (Pre1), Bi2tos (Pre2), a synbiotic composed Lactococcus lactis subsp. IBB SL1 (Syn1), L. cremoris SC1 (Syn2), physiological saline (control...

Summary Four domesticated strains of Nile tilapia ( Oreochromis niloticus L.) were genetically characterized using 14 microsatellite markers and 64 animals per strain. Two strains, Chitralada (AIT) International Development Research Centers (IDRC) obtained from the AIT institute, Bangkok, Thailand. The GIFT strain (5th generation) came NAGRI, Thailand, GÖTT was supplied by University Göttingen, Germany. average numbers alleles marker 5.0 (GÖTT), 5.4 (AIT), 5.6 7.5 (GIFT). Private found at...

The Green-legged Partridgelike (GP) fowl, an old native Polish breed, is characterised by reseda green-coloured shanks rather than yellow, white, slate or black commonly observed across most domestic breeds of chicken. Here, we investigate the origin, genetic relationships and structure GP fowl using mtDNA D-loop sequencing genome-wide SNP analysis. Genome-wide association analysis between enables us to verify control green shank phenotype, a defining trait for breed. Two haplogroups three...

Galactooligosaccharides (GOS) that are delivered in ovo improve intestinal microbiota composition and mitigate the negative effects of heat stress broiler chickens. Hubbard hybrids slow-growing chickens with a high resistance to heat. In this paper, we determined impact GOS on challenged The experiment was 2 × factorial design. On day 12 incubation, (3.5 mg/egg) into egg (n = 300). Controls (C) were mock-injected physiological saline After hatching, C groups split thermal groups:...

Abstract Campylobacter and Salmonella are the two most prominent foodborne zoonotic pathogens reported in European Union. As poultry is one of major sources these pathogens, it imperative to mitigate colonization poultry. Many strains lactic acid bacteria (LAB) have demonstrated anti- characteristics varying degrees spectrums which attributed production various metabolites. However, compounds consequent antimicrobial properties highly strain dependent. Therefore, current study was performed...

Feather pecking (FP) is a major problem in cage and free-range housing systems. In systems, FP more difficult to control. It not known why victim being pecked. could be that bird genetically predisposed To study the genetics of behavior, large F2 population 630 hens was generated from cross between two commercial laying lines differing their propensity feather peck. The traits measured at 6 30 wk age were receiving gentle FP, severe aggressive pecking. addition, toe (TP) also age. For age,...

The aim of the study was to determine effect probiotics, prebiotics and synbiotics administered in ovo on selected morphological parameters small intestine (duodenum, jejunum, ileum) broiler chickens (Ross 308) native (Green-legged Partridge, GP). On 12th day embryonic development (the incubation period), an aqueous solution a suitable bioactive substance supplied egg's air cell: probiotic-Lactococcus lactis subsp. cremoris (PRO), prebiotic-GOS, galacto-oligosaccharides (PRE) or...

We describe the mapping of amplified restriction fragment polymorphism (AFLP) markers in chicken (Gallus domesticus) using a multi-colour fluorescent detection system. DNA was used from population consisting four families with total 183 F2 individuals. The enzyme combination EcoRI/TaqI for double digestion, and fluorescently labelled fragments were analysed on an ABI PRISM 377 sequencer. Polymorphic signals range 50-500 bp genotyped Genotyper 2.0 software, which enabled analysis both...