A5069916411- Genomics and Chromatin Dynamics
- SARS-CoV-2 and COVID-19 Research
- Acute Lymphoblastic Leukemia research
- COVID-19 Clinical Research Studies
- Microtubule and mitosis dynamics
- Bioinformatics and Genomic Networks
- SARS-CoV-2 detection and testing
- RNA and protein synthesis mechanisms
- Cancer Genomics and Diagnostics
- Childhood Cancer Survivors' Quality of Life
- Single-cell and spatial transcriptomics
- Receptor Mechanisms and Signaling
- CRISPR and Genetic Engineering
- COVID-19 and healthcare impacts
- Estrogen and related hormone effects
- Gene expression and cancer classification
- Prenatal Screening and Diagnostics
- RNA Research and Splicing
- Respiratory viral infections research
- Chromosomal and Genetic Variations
- RNA modifications and cancer
- Biotin and Related Studies
- BRCA gene mutations in cancer
- Genomics and Phylogenetic Studies
Centre Hospitalier Universitaire Sainte-Justine
2021-2024
Institute for Research in Immunology and Cancer
2012-2018
Université de Montréal
2012-2018
Stanford University
2013
Meertens Institute
2009
The Netherlands Cancer Institute
2005
United States Office of Personnel Management
2005
Oncode Institute
2005
Kinetochore Targeting Chromosomes must be segregated accurately during cell division. This is facilitated by the attachment of mitotic spindle microtubules to kinetochore at chromosomal centromere. The centromere marked with histone H3 variant CenH3 (CENP-A in human), and CENP-C forms part inner kinetochore. Kato et al. (p. 1110 ) used structural biology, biochemistry, mutagenesis show that recognizes CENP-A chromatin via several different interactions. "central domain" makes close contact...
Abstract Here we present the Transcription Factor Encyclopedia (TFe), a new web-based compendium of mini review articles on transcription factors (TFs) that is founded principles open access and collaboration. Our consortium over 100 researchers has collectively contributed 130 pertinent human, mouse rat TFs. Notable features TFe website include high-quality PDF generator web API for programmatic data retrieval. aims to rapidly educate scientists about TFs they encounter through delivery...
Polo-like kinase 1 (Plk1) plays a role in numerous events mitosis, but how the multiple functions of Plk1 are separated is poorly understood. We studied regulation through two putative phosphorylation residues, Ser-137 and Thr-210. Using phospho-specific antibodies, we found that Thr-210 precedes vivo, latter occurring specifically late mitosis. show expression activating mutants these S137D T210D, results distinct mitotic phenotypes. Whereas both phospho-mimicking as well double mutant...
Genome-wide transcriptome profiling has enabled non-supervised classification of tumours, revealing different sub-groups characterized by specific gene expression features. However, the biological significance these subtypes remains for most part unclear. We describe herein an interactive platform, Minimum Spanning Trees Inferred Clustering (MiSTIC), that integrates direct visualization and comparison correlation structure between datasets, analysis molecular causes underlying co-variations...
Significance Many biological processes, including signal transduction pathways and gene expression regulation, require the assembly of multisubunit protein complexes in a temporally coordinated fashion. However, approaches to studying dynamics properties multimeric complex currently remain limited. We have developed method monitor trimeric live cells, using specific combination bioluminescence- fluorescence-based energy transfer. illustrate potential this technology reveal ligand-activated...
Abstract Resolving the transcriptomes of higher eukaryotes is more tangible with advent long read sequencing, which greatly facilitates identification new transcripts and their splicing isoforms. However, computational analysis RNA sequencing data remains challenging as it difficult to disentangle technical artifacts from bona fide biological information. To address this, we evaluated performance multiple leading transcriptome assembly algorithms on ability accurately reconstruct transcript...
Abstract Gene expression profiling provides a detailed molecular snapshot of cellular phenotypes that can be used to compare different biological conditions. Nanopore sequencing technology generate high-resolution transcriptomic data in real-time and at low cost, which heralds new opportunities for medicine. In this study, we demonstrate the clinical utility by processing RNA from childhood acute lymphoblastic leukemia (ALL) patients on-the-fly with trained neural network classifier. This...
The first confirmed case of COVID-19 in Quebec, Canada, occurred at Verdun Hospital on February 25, 2020. A month later, a localized outbreak was observed this hospital. We performed tiled amplicon whole genome nanopore sequencing nasopharyngeal swabs from all SARS-CoV-2 positive samples 31 March to 17 April 2020 2 local hospitals assess viral diversity (unknown the time Quebec) and potential associations with clinical outcomes. report 264 genomes 242 individuals-both staff patients-with...
BACKGROUND: COVID-19 is usually a time-limited disease. However, prolonged infections and reinfections can occur among immunocompromised patients. It be difficult to distinguish infection from new one, especially when reinfection occurs early. METHODS: We report the case of 57-year-old man infected with SARS-CoV-2 while undergoing chemotherapy for follicular lymphoma. He experienced symptomatic 3 months despite 5-day course remdesivir eventually deteriorated died. RESULTS: Viral genome...
Abstract Background The objective of this study is to assess the concordance and added value combined comparative genomic hybridization plus single‐nucleotide polymorphism microarray (CGH/SNP) analyses in pediatric acute lymphoblastic leukemia (ALL) risk stratification compared conventional cytogenetic methods. Procedure This a retrospective that included patients aged 1–18 years diagnosed with de novo ALL at Sainte‐Justine Hospital between 2016 2021. Results from molecular were collected...
The first confirmed case of COVID-19 in Quebec, Canada, occurred at Verdun Hospital on February 25, 2020. A month later, a localized outbreak was observed this hospital. We performed tiled amplicon whole genome nanopore sequencing nasopharyngeal swabs from all SARS-CoV-2 positive samples 31 March to 17 April 2020 2 local hospitals assess the viral diversity outbreak. report 264 genomes 242 individuals (both staff and patients) with associated clinical features outcomes, as well longitudinal...