A5090488719- FOXO transcription factor regulation
- Pregnancy and preeclampsia studies
- Cancer, Lipids, and Metabolism
- Microtubule and mitosis dynamics
- Chemotherapy-induced cardiotoxicity and mitigation
- Endometriosis Research and Treatment
- Ovarian cancer diagnosis and treatment
- Circular RNAs in diseases
- Reproductive System and Pregnancy
- Cancer-related molecular mechanisms research
- Birth, Development, and Health
- PARP inhibition in cancer therapy
- Cancer-related Molecular Pathways
- Gestational Diabetes Research and Management
- MicroRNA in disease regulation
- Extracellular vesicles in disease
- Metabolism, Diabetes, and Cancer
- DNA Repair Mechanisms
- Sirtuins and Resveratrol in Medicine
- Cell death mechanisms and regulation
- Genomics and Chromatin Dynamics
- Genetics, Aging, and Longevity in Model Organisms
- Neonatal Respiratory Health Research
- Disaster Response and Management
- CRISPR and Genetic Engineering
Universidad de Los Andes, Chile
2015-2024
Cancer Research UK
2009-2023
University of Chile
2016
Hammersmith Hospital
2010-2015
Imperial College London
2009-2015
Universitat de Barcelona
2011-2012
Ludwig Cancer Research
2011
University of Hong Kong
2011
Sichuan University
2011
West China Hospital of Sichuan University
2011
Endometrial cancer is the most common malignancy of lower female reproductive tract. The tumor suppressor FOXO1 downregulated in endometrial compared with normal endometrium but underlying mechanisms are not well understood. Using microRNA (miR) target prediction algorithms, we identified several miRs that potentially bind 3'-untranslated region transcripts. Expression profiling and malignant samples by quantitative real-time PCR Northern blot analysis revealed an inverse correlation between...
FOXM1 has been implicated in taxane resistance, but the molecular mechanism involved remains elusive. In here, we show that depletion can sensitize breast cancer cells and mouse embryonic fibroblasts into entering paclitaxel-induced senescence, with loss of clonogenic ability, induction senescence-associated β-galactosidase activity flat cell morphology. We also demonstrate regulates expression microtubulin-associated kinesin KIF20A at transcriptional level directly through a Forkhead...
The transcription factor Forkhead box M1 (FOXM1) is a key regulator of cell proliferation and overexpressed in many forms primary cancers, leading to uncontrolled division genomic instability. To address the role FOXM1 chemoresistance, we generated cisplatin-resistant breast cancer line (MCF-7-CIS(R)), which had an elevated level protein mRNA expression relative parental MCF-7 cells. A close correlation was observed between its proposed downstream targets that are involved DNA repair;...
In this report, we investigated the role and regulation of forkhead box M1 (FOXM1) in breast cancer epirubicin resistance. We generated epirubicin-resistant MCF-7 carcinoma (MCF-7-EPI(R)) cells found FOXM1 protein levels to be higher MCF-7-EPI(R) than that expression is downregulated by but not cells. also established there a loss p53 function represses at transcription gene promoter through activation repression E2F activity Using p53(-/-) mouse embryo fibroblasts, showed important for...
In this study, we report the identification of a novel role SIRT6 in both epirubicin and paclitaxel resistance breast cancer. We found that protein levels are elevated paclitaxel- epirubicin-resistant MCF-7 cells compared with parental sensitive cells. knockout depletion sensitized to treatment, whereas ectopic overexpression led increased epirubicin. Moreover, our data suggest could be mediating through enhancing DNA repair response epirubicin-induced damage. Clonogenic assays also revealed...
Abstract Here we present the Transcription Factor Encyclopedia (TFe), a new web-based compendium of mini review articles on transcription factors (TFs) that is founded principles open access and collaboration. Our consortium over 100 researchers has collectively contributed 130 pertinent human, mouse rat TFs. Notable features TFe website include high-quality PDF generator web API for programmatic data retrieval. aims to rapidly educate scientists about TFs they encounter through delivery...
FOXO3a is a forkhead transcription factor that regulates multitude of important cellular processes, including proliferation, apoptosis, differentiation, and metabolism. Doxorubicin treatment MCF-7 breast carcinoma cells results in nuclear relocation the induction stress-activated kinase p38 MAPK. Here, we studied potential regulation by response to doxorubicin. Co-immunoprecipitation studies demonstrated direct interaction between FOXO3a. We also showed can bind phosphorylate recombinant...
FOXM1 is implicated in genotoxic drug resistance but its mechanism of action remains elusive. We show here that FOXM1-depletion can sensitize breast cancer cells and mouse embryonic fibroblasts (MEFs) into entering epirubicin-induced senescence, with the loss long-term cell proliferation ability, accumulation γH2AX foci, induction senescence-associated β-galactosidase activity morphology. Conversely, reconstitution FOXM1-deficient MEFs alleviates foci. also demonstrate regulates NBS1 at...
The PI3K-Akt signal pathway plays a key role in tumorigenesis and the development of drug-resistance. Cytotoxic chemotherapy resistance is linked to limited therapeutic options poor prognosis.Examination FOXO3a phosphorylated-Akt (P-Akt) expression breast cancer tissue microarrays showed nuclear was associated with lymph node positivity (p = 0.052), prognosis 0.014), P-Akt invasive ductal carcinoma. Using tamoxifen doxorubicin-sensitive -resistant cell lines as models, we found that...
Introduction Preeclampsia is a maternal hypertensive disorder with uncertain etiology and leading cause of fetal mortality worldwide, causing nearly 40% premature births delivered before 35 weeks gestation. The first stage preeclampsia characterized by reduction utero-placental blood flow which reflected in high pressure proteinuria during the second half pregnancy. In human placenta androgens derived from adrenal glands are converted into estrogens enzymatic action placental aromatase. This...
iASPP is a specific regulator of p53-mediated apoptosis. Herein, we provided the first report on expression profile in ovarian epithelial tumor and its effect paclitaxel chemosensitivity.Expression amplification status was examined 203 clinical samples 17 cell lines using immunohistochemistry, quantitative real-time PCR, immunoblotting, correlated with clinicopathologic parameters. Changes proliferation, mitotic catastrophe, apoptosis, underlying mechanism cancer cells different p53...
E2F1 is responsible for the regulation of FOXM1 expression, which plays a key role in epirubicin resistance. Here, we examined and response to cancer cells. We first showed that promoting cell survival, resistance as its depletion by siRNA attenuated induction viability epirubicin. also found p38-MAPK activity mirrors expression patterns both epirubicin-sensitive -resistant MCF-7 breast cells, suggesting p38 has regulating Consistently, studies using pharmacologic inhibitors, knockdown,...
FOXA1 expression correlates with the breast cancer luminal subtype and patient survival. RNA protein analysis of a panel cell lines revealed that BRCA1 deficiency is associated downregulation expression. Knockdown resulted in enhancement promoter methylation MCF-7 cells, whereas reconstitution Brca1-deficent mouse mammary epithelial cells (MMECs) promoted Foxa1 methylation. These data suggest suppresses hypermethylation silencing. Consistently, treatment MMECs DNA inhibitor...
Natural disasters increase the level population stress, including pregnant women, who can experience prenatal maternal affecting fetus and triggering perinatal complications, such as low birth weight, smaller head circumference, etc. However, little is known about effects of earthquake on outcomes.To evaluate effect occurred February 27, 2010 outcomes Chilean to examine these by timing exposure during pregnancy newborn gender.A register-based study was performed using data collected from...
Aim To isolate and characterize oral extracellular vesicles from gingival crevicular fluid at 11–14 weeks evaluate their capacity to identify patients risk of developing gestational diabetes mellitus. Methods A case-control study was conducted, including who developed mellitus (n = 11) healthy pregnant controls 23). Obstetric periodontal histories were recorded gestation, samples obtained. Extracellular isolated by ExoQuick. Nanoparticle tracking analysis, ELISA transmission electron...
Successful implantation requires coordinated migration and invasion of trophoblast cells into a receptive endometrium. Reduced forkhead box M1 (FOXM1) expression limits angiogenesis in choriocarcinoma cell lines, rat model, placental FOXM1 protein was significantly upregulated the early stages pregnancy compared to term pregnancy. However, precise role events remains unknown. By analyzing mice blastocysts at embryonic day (E3.5), we have demonstrated that is expressed as blastocyst stage, it...
During gestation, low oxygen environment is a major determinant of early placentation process, while persistent placental hypoxia leads to pregnancy-related complications such as preeclampsia (PE) and intrauterine growth restriction (IUGR). PE affects 5%-8% all pregnancies worldwide cause maternal fetal morbidity mortality. development, due poor trophoblast invasion reduced uteroplacental perfusion endothelial dysfunction clinical manifestation PE. Here we hypothesized that nuclear factor...
To explore the diagnostic usefulness of extracellular vesicles (EVs), and their subpopulations (micro-vesicles exosomes), microRNAs (miRNA-21-3p, miRNA-150-5p, miRNA-26a-5p) in peri-implant crevicular fluid (PICF) subjects with healthy, mucositis peri-implantitis implants.A total 54 patients were enrolled into mucositis, groups. PICF samples collected, EVs (MVs Exo) isolated characterized by nanoparticle tracking analysis transmission electron microscopy. The expression miRNA-21-3p,...