A5088904321- Neuroscience and Neuropharmacology Research
- Extracellular vesicles in disease
- Neurogenesis and neuroplasticity mechanisms
- MicroRNA in disease regulation
- RNA Interference and Gene Delivery
- Neuroinflammation and Neurodegeneration Mechanisms
- Tryptophan and brain disorders
- Nerve injury and regeneration
- Memory and Neural Mechanisms
- Stress Responses and Cortisol
- interferon and immune responses
- Neural dynamics and brain function
- Ion channel regulation and function
- Circular RNAs in diseases
- Sleep and Wakefulness Research
- Circadian rhythm and melatonin
- Adipose Tissue and Metabolism
- Nicotinic Acetylcholine Receptors Study
- Neuroendocrine regulation and behavior
- Photoreceptor and optogenetics research
- Redox biology and oxidative stress
- Epilepsy research and treatment
- Nitric Oxide and Endothelin Effects
- Fungal and yeast genetics research
- Protein Tyrosine Phosphatases
Universidad de Los Andes, Chile
2015-2024
Universidad de Los Andes
2014-2024
Advanced Center for Chronic Diseases
2023
Clínica Las Condes
2017
University of Chile
1992-2015
Universidad Andrés Bello
2015
Karolinska Institutet
2015
Universidad de Los Andes
2003-2013
Universidad Católica del Norte
2011
Leibniz Institute for Neurobiology
2000-2001
The Dlg4 gene encodes for post-synaptic density protein 95 (PSD95), a major synaptic that clusters glutamate receptors and is critical plasticity. PSD95 levels are diminished in ageing neurodegenerative disorders, including Alzheimer's disease Huntington's disease. epigenetic mechanisms (dys)regulate transcription of Dlg4/PSD95, or other plasticity genes, largely unknown, limiting the development targeted epigenome therapy. We analysed Dlg4/PSD95 landscape hippocampal tissue designed...
Astrocytes use gliotransmitters to modulate neuronal function and plasticity. However, the role of small extracellular vesicles, called exosomes, in astrocyte-to-neuron signaling is mostly unknown. Exosomes originate multivesicular bodies parent cells are secreted by fusion body limiting membrane with plasma membrane. Their molecular cargo, consisting RNA species, proteins, lipids, part cell type state specific. Among species transported microRNAs (miRNAs) able modify gene expression...
Aging compromises brain function leading to cognitive decline. A cyclic ketogenic diet (KD) improves memory in aged mice after long-term administration; however, short-term effects later life and the molecular mechanisms that govern such changes remain unclear. Here, we explore impact of a KD treatment starting at elderly stage on mice. Behavioral testing potentiation (LTP) recordings reveal working hippocampal LTP. Furthermore, synaptosome proteome fed evidence predominantly presynaptic...
Stress precipitates mood disorders, characterized by a range of symptoms present in different combinations, suggesting the existence disease subtypes. Using an animal model, we previously described that repetitive stress via restraint or immobilization induced depressive-like behaviors rats were differentially reverted serotonin- noradrenaline-based antidepressant drug, indicating neurobiological mechanisms may be involved. The forebrain astrocyte protein aldolase C, contained small...
In the last few decades, it has been established that astrocytes play key roles in regulation of neuronal morphology. However, contribution astrocyte-derived small extracellular vesicles (sEVs) to morphological differentiation neurons only recently addressed. Here, we showed cultured expressing a GFP-tagged version stress-regulated astrocytic enzyme Aldolase C (Aldo C-GFP) release are transferred into hippocampal neurons. Surprisingly, Aldo C-GFP-containing sEVs C-GFP sEVs) displayed an...
Neuroplastin-65 and -55 (previously known as gp65 gp55) are glycoproteins of the Ig superfamily that enriched in rat forebrain synaptic membrane preparations. Whereas two-Ig domain isoform neuroplastin-55 is expressed many tissues, three-Ig neuroplastin-65 brain-specific postsynaptic density (PSD) protein Here, we have assessed function neuroplastin long-term plasticity. Immunocytochemical studies with neuroplastin-65-specific antibodies differentially stain distinct neuropil regions...
It is thought that the ability of human mesenchymal stem cells (hMSC) to deliver neurotrophic factors might be potentially useful for treatment neurodegenerative disorders. The aim present study was characterize signals and/or molecules regulate brain-derived factor (BDNF) protein expression/delivery in hMSC cultures and evaluate effect epigenetically generated BDNF-secreting on intact lesioned substantia nigra (SN). We tested 4 different culture media found presence fetal bovine serum (FBS)...
Clinically depressed individuals respond to different types of antidepressants, suggesting that neurobiological mechanisms may be responsible for their depression. However, animal models characterize this are not yet available. We induced depressive-like behaviors in rats using 2 chronic stress models: restraint small cages or immobilization adaptable plastic cones. Both increased anxiety responses evaluated by novelty-suppressed feeding and the elevated plus-maze; learned helplessness tail...
Among the mechanisms of suppression that T regulatory (Treg) cells exert to control immune responses, secretion small extracellular vesicles (sEV) has been recently proposed as a novel contact-independent immunomodulatory mechanism. Previous studies have demonstrated Treg produce sEV, including exosomes, able modulate effector function CD4+ cells, and antigen presenting (APCs) such dendritic (DCs) through transfer microRNA, cytokines, production adenosine, among others. Previously, we...
ORIGINAL RESEARCH article Front. Cell. Neurosci., 09 May 2013Sec. Cellular Neurophysiology https://doi.org/10.3389/fncel.2013.00066
Methadone is a synthetic long-acting opioid that increasingly used in the replacement therapy of opioid-addicted patients, including pregnant women. However, methadone this population poses challenges, as it induces cognitive and behavioral impairments infants exposed to during prenatal development. In animal models, exposure results detrimental consequences central nervous system, such as: (i) increased neuronal apoptosis; (ii) disruption oligodendrocyte maturation apoptosis (iii) microglia...
1. Sixteen rats were recorded continuously for 3 days using an automated system that detected, quantified, and stored the incidence of cortical delta waves, sigma spindles, hippocampal theta rhythm, electromyographic activity. A time series then was constructed wherein 15-s epochs ascribed to one behavioral state: wakefulness (W), quiet sleep (QS), or active (AS, a state also referred as REM sleep). From those series, AS episodes non-AS intervals could be determined. Episodes defined lasting...
Nitric oxide exerts important regulatory functions in various brain processes. Its synthesis neurons has been most commonly ascribed to the neuronal nitric synthase (nNOS) isoform. However, endothelial isoform (eNOS), which is significantly associated with caveolae different cell types, implicated synaptic plasticity and enriched dendrites of CA1 hippocampal neurons. Using high resolution microscopy co-distribution analysis eNOS raft proteins, we now show for first time primary cortical...
Chronic stress affects brain areas involved in learning and emotional responses. Although most studies have concentrated on the effect of limbic-related structures, this study we investigated whether chronic might induce impairments diencephalic structures associated with limbic components response. Specifically, analyzed immobilization expression sympathetic markers rat epithalamic pineal gland by immunohistochemistry western blot, whereas plasma melatonin concentration was determined...