A5072131030- Extracellular vesicles in disease
- MicroRNA in disease regulation
- Pregnancy and preeclampsia studies
- Toxoplasma gondii Research Studies
- Ubiquitin and proteasome pathways
- RNA Interference and Gene Delivery
- Immunotherapy and Immune Responses
- Epigenetics and DNA Methylation
- Birth, Development, and Health
- Marine Biology and Ecology Research
- Cancer, Hypoxia, and Metabolism
- Autophagy in Disease and Therapy
- Cytomegalovirus and herpesvirus research
- Nitric Oxide and Endothelin Effects
- Immune cells in cancer
- Adenosine and Purinergic Signaling
- Endoplasmic Reticulum Stress and Disease
- Cell Adhesion Molecules Research
- Caveolin-1 and cellular processes
- RNA regulation and disease
- Gestational Diabetes Research and Management
- Cancer-related molecular mechanisms research
- Neurogenesis and neuroplasticity mechanisms
- Herpesvirus Infections and Treatments
- Axon Guidance and Neuronal Signaling
Advanced Center for Chronic Diseases
2022-2025
University of Chile
2006-2025
Fundación Ciencia and Vida
2011-2024
San Sebastián University
2019-2024
Biogipuzkoa Health Research Institute
2024
University College London
2024
Universidad Mayor
2024
Centro de Recursos Educativos Avanzados
2023
Karolinska Institutet
2014-2022
Universidad de Los Andes, Chile
2015-2020
The early and long-term effects of coronary artery ligation on the plasma left ventricular angiotensin-converting enzyme (ACE ACE2) activities, ACE ACE2 mRNA levels, circulating angiotensin (Ang) levels [Ang I, Ang-(1-7), Ang-(1-9), Ang II], cardiac function were evaluated 1 8 weeks after experimental myocardial infarction in adult Sprague Dawley rats. Sham-operated rats used as controls. Coronary caused infarction, hypertrophy, dysfunction surgery. At week 1, II Ang-(1-9) well activities...
Extracellular vesicles (EVs) have shown great potential for targeted therapy, as they a natural ability to pass through biological barriers and, depending on their origin, can preferentially accumulate at defined sites, including tumors. Analyzing the of EVs target specific cells remains challenging, considering unspecific binding lipophilic tracers other proteins, limitations fluorescence deep tissue imaging and effect external labeling strategies tropism. In this work, we determined...
Abstract In the adult hippocampus, new neurons are generated in dentate gyrus. The Wnt signaling pathway regulates this process, but little is known about endogenous ligands involved. We investigated role of Wnt5a on hippocampal neurogenesis. neuronal morphogenesis during embryonic development, and maintains dendritic architecture pyramidal hippocampus. Here, we determined that knockdown mouse gyrus by lentivirus-mediated shRNA impaired differentiation progenitor cells, reduced development...
Disruption of the class I human leukocyte antigen (HLA) molecules has important implications for immune evasion and tumor evolution. We developed major histocompatibility complex loss heterozygosity (LOH), allele-specific mutation measurement expression repression (MHC Hammer). identified extensive variability in HLA allelic pervasive alternative splicing normal lung breast tissue. In TRACERx TCGA cohorts, 61% adenocarcinoma (LUAD), 76% squamous cell carcinoma (LUSC) 35% estrogen...
Angiotensin-(1-9) is present in human and rat plasma its circulating levels increased early after myocardial infarction or animals treated with angiotensin-converting enzyme inhibitor. However, the cardiovascular effects of this peptide are unknown.To determine whether angiotensin-(1-9) a novel anti-cardiac hypertrophy factor vitro vivo involved pharmacological drugs acting on renin-angiotensin system.The administration to infarcted rats by osmotic minipumps (450 ng/kg per min, n = 6) vs....
Mesenchymal stem cells (MSCs) secrete exosomes that are capable of modifying the tumor environment through different mechanisms including changes in cancer-cell secretome. This activity depends on their cargo content is largely defined by cellular origin. Endometrial fine regulators angiogenic process during menstrual cycle includes an angiostatic condition associated with end cycle. Hence, we studied (MenSCs)-secreted prostate PC3 cells. Our results showed induce a reduction VEGF secretion...
Stress precipitates mood disorders, characterized by a range of symptoms present in different combinations, suggesting the existence disease subtypes. Using an animal model, we previously described that repetitive stress via restraint or immobilization induced depressive-like behaviors rats were differentially reverted serotonin- noradrenaline-based antidepressant drug, indicating neurobiological mechanisms may be involved. The forebrain astrocyte protein aldolase C, contained small...
In the adult hippocampus new neurons are continuously generated from neural stem cells (NSCs) present at subgranular zone of dentate gyrus. This process is controlled by Wnt signaling, which plays a complex role in regulating multiple steps neurogenesis including maintenance, proliferation and differentiation progenitor development newborn neurons. Differential effects signaling during progression could be mediated cell-type specific expression receptors. Here we studied potential Frizzled-1...
Breast cancer is one of the most frequently diagnosed cancers and leading cause cancer-related deaths in women worldwide, whereby mortality largely attributable to development distant metastasis. Caveolin-1 (CAV1) a multifunctional membrane protein that typically upregulated final stages promotes migration invasion tumor cells. Elevated levels CAV1 have been detected extracellular vesicles (EVs) from advanced patients. EVs are lipid enclosed vesicular structures contain bioactive proteins,...
Memory CD8+ T cell responses have the potential to mediate long-lasting protection against cancers. Resident memory (Trm) cells stably reside in non-lymphoid tissues and superior innate adaptive immunity pathogens. Emerging evidence indicates that Trm develop human solid cancers play a key role controlling tumor growth. However, specific contribution of anti-tumor is incompletely understood. Moreover, clinically applicable vaccination strategies efficiently establish remain largely...
Introduction Preeclampsia is a maternal hypertensive disorder with uncertain etiology and leading cause of fetal mortality worldwide, causing nearly 40% premature births delivered before 35 weeks gestation. The first stage preeclampsia characterized by reduction utero-placental blood flow which reflected in high pressure proteinuria during the second half pregnancy. In human placenta androgens derived from adrenal glands are converted into estrogens enzymatic action placental aromatase. This...
Abstract Protein kinase CK2 is a highly conserved and constitutively active Ser/Thr-kinase that phosphorylates large number of substrates, resulting in increased cell proliferation survival. A known target Akt, player the PI3K/Akt/mTORC1 signaling pathway, which aberrantly activated 32% colorectal cancer (CRC) patients. On other hand, mTORC1 plays an important role regulation protein synthesis, growth, autophagy. Some studies suggest regulates several cancers. The most recently developed...
In the last few decades, it has been established that astrocytes play key roles in regulation of neuronal morphology. However, contribution astrocyte-derived small extracellular vesicles (sEVs) to morphological differentiation neurons only recently addressed. Here, we showed cultured expressing a GFP-tagged version stress-regulated astrocytic enzyme Aldolase C (Aldo C-GFP) release are transferred into hippocampal neurons. Surprisingly, Aldo C-GFP-containing sEVs C-GFP sEVs) displayed an...
Clinically depressed individuals respond to different types of antidepressants, suggesting that neurobiological mechanisms may be responsible for their depression. However, animal models characterize this are not yet available. We induced depressive-like behaviors in rats using 2 chronic stress models: restraint small cages or immobilization adaptable plastic cones. Both increased anxiety responses evaluated by novelty-suppressed feeding and the elevated plus-maze; learned helplessness tail...
The obligate intracellular parasite Toxoplasma gondii exploits cells of the immune system to disseminate. Upon T. gondii-infection, γ-aminobutyric acid (GABA)/GABAA receptor signaling triggers a hypermigratory phenotype in dendritic (DCs) by unknown signal transduction pathways. Here, we demonstrate that calcium (Ca2+) DCs is indispensable for gondii-induced DC hypermotility and transmigration vitro. We report activation GABAA receptors GABA induces transient Ca2+ entry DCs. Murine bone...
The obligate intracellular parasite Toxoplasma gondii exploits cells of the immune system to disseminate. Upon infection, parasitized dendritic (DCs) and microglia exhibit a hypermigratory phenotype in vitro that has been associated with enhancing dissemination vivo mice. One unresolved question is how parasites commandeer achieve systemic by 'Trojan-horse' mechanism. By chromatography mass spectrometry analyses, we identified an orthologue 14-3-3 protein family, T. (Tg14-3-3), as mediator...
Introduction: Extracellular vesicles (EVs) are secreted from all types of cells and involved in the trafficking proteins, metabolites, genetic material cell to cell. According their biogenesis physical properties, EVs often classified as small (including exosomes) or large EVs, oncosomes. A variety methods used for isolated EVs; however, they have several limitations, including vesicle deformation, reduced particle yield, co-isolate protein contaminants. Here we present an optimized fast...
Membrane vesicles (MVs) are envelope-derived extracellular sacs that perform a broad diversity of physiological functions in bacteria. While considerably studied pathogenic microorganisms, the roles, relevance, and biotechnological potential MVs from environmental bacteria less well established. Acidithiobacillaceae family active players sulfur iron biogeochemical cycles extremely acidic environments drivers leaching mineral ores contributing to acid rock/mine drainage (ARD/AMD) industrial...
Caveolin-1 (CAV1) is a membrane protein that promotes migration, invasion and metastasis of cancer cells when phosphorylated on tyrosine-14 (Y14) by cell intrinsic mechanism involving the activation novel Rab5-Rac1 signaling axis. Moreover, CAV1 expressed in aggressive included into extracellular vesicles (EVs) such EVs increase metastatic potential recipient lower grade cells. However, relevance Y14 phosphorylation these extrinsic EV-stimulated events remained to be determined. Here we used...
// Lorena Lobos-González 1, 2, 3 , Verónica Silva 2 Mariela Araya Franko Restovic 8 Javiera Echenique Luciana Oliveira-Cruz Christopher Fitzpatrick 4 Macarena Briones Jaime Villegas Claudio Villota Soledad Vidaurre 5 Vincenzo Borgna 6 Miguel Socias 7 Sebastián Valenzuela Constanza Lopez Teresa Manuel Varas Jorge Díaz Luis O. Burzio A. 1 Andes Biotechnologies SpA, Santiago, Chile Fundación Ciencia & Vida, Facultad de Medicina, Universidad Chile, Independencia, Ciencias Biológicas, Andrés...