A5035834555- Metal Extraction and Bioleaching
- Minerals Flotation and Separation Techniques
- Corrosion Behavior and Inhibition
- Genomics and Phylogenetic Studies
- Arsenic contamination and mitigation
- Electronic and Structural Properties of Oxides
- Bacteriophages and microbial interactions
- Trace Elements in Health
- Metalloenzymes and iron-sulfur proteins
- Microbial Community Ecology and Physiology
- Cassava research and cyanide
- Mineral Processing and Grinding
Fundación Ciencia and Vida
2020-2024
San Sebastián University
2021-2024
Millennium Science Initiative
2021
University of Chile
2004-2011
University of Antofagasta
2011
Millennium Institute for Integrative Biology
2004-2005
ABSTRACT A set of proteins that changed their levels synthesis during growth Acidithiobacillus ferrooxidans ATCC 19859 on metal sulfides, thiosulfate, elemental sulfur, and ferrous iron was characterized by using two-dimensional polyacrylamide gel electrophoresis. N-terminal amino acid sequencing mass spectrometry analysis these allowed identification the localization corresponding genes in available genomic sequence A. 23270. The context around several suggests involvement energetic...
Abstract Members of the genus Acidithiobacillus, now ranked within class Acidithiobacillia, are model bacteria for study chemolithotrophic energy conversion under extreme conditions. Knowledge genomic and taxonomic diversity Acidithiobacillia is still limited. Here, we present a systematic analysis nearly 100 genomes from sampled wide range habitats. Some these new others have been reclassified on basis advanced analysis, thus defining 19 lineages ranking at different levels. This work...
Acidithiobacillus ferrooxidans is a chemolithoautotrophic acidophile capable of obtaining energy by oxidizing ferrous iron or sulfur compounds such as metal sulfides. Some the proteins involved in these oxidations have been described forming part periplasm this extremophile. The detailed study periplasmic components constitutes an important area to understand physiology and environmental interactions microorganisms. Proteomics analysis fraction A. ATCC 23270 was performed using high...
Membrane vesicles (MVs) are envelope-derived extracellular sacs that perform a broad diversity of physiological functions in bacteria. While considerably studied pathogenic microorganisms, the roles, relevance, and biotechnological potential MVs from environmental bacteria less well established. Acidithiobacillaceae family active players sulfur iron biogeochemical cycles extremely acidic environments drivers leaching mineral ores contributing to acid rock/mine drainage (ARD/AMD) industrial...
Plasmids are major drivers of microbial evolution, enabling horizontal gene transfer and facilitating adaptation through the dissemination relevant genes traits. Little is known about plasmid diversity function in extremophiles. 'Fervidacidithiobacillus caldus' a meso-thermo-acidophilic sulfur oxidizer, key player cycling acidic environments. Here, we present comprehensive analysis plasmidome, associated anti-mobile genetic element defense systems, across genomes this species metagenomes...
Production of sulfur globules during sulfide or thiosulfate oxidation is a characteristic feature some bacteria. Although their generation has been reported in Acidithiobacillus ferrooxidans, its mechanism formation and deposition, as well the physiological significance these compounds oxidation, are currently unknown. Under oxygen sufficient conditions (OSC), A. ferrooxidans oxidizes to tetrathionate, which accumulates culture medium. Tetrathionate then oxidized by tetrathionate hydrolase...
Eight nucleotide sequences containing a single rhodanese domain were found in the Acidithiobacillus ferrooxidans ATCC 23270 genome: p11, p14, p14.3, p15, p16, p16.2, p21, and p28. Amino acids sequence comparisons allowed us to identify potentially catalytic Cys residues other highly conserved family features all eight proteins. The genomic contexts of some rhodanese-like genes determination their expression at mRNA level by using macroarrays suggested implication sulfur oxidation metabolism,...
Abstract The recent revision of the Acidithiobacillia class using genomic taxonomy methods has shown that, in addition to existence previously unrecognized genera and species, some species harbor levels divergence that are congruent with ongoing differentiation processes. In this study, we have performed a subspecies-level analysis sequenced strains Acidithiobacillus ferrooxidans prove distinct sublineages identify discriminant genomic/genetic characteristics linked these sublineages, shed...
Mobile genetic elements (MGEs) are relevant agents in bacterial adaptation and evolutionary diversification. Stable appropriation of these DNA depends on host factors, among which the nucleoid-associated proteins (NAPs). NAPs highly abundant that bind bend DNA, altering its topology folding, thus affecting all known cellular processes from replication to expression. Even though NAP coding genes found most prokaryotic genomes, their functions chromosome biology xenogeneic silencing only for a...