- Metal Extraction and Bioleaching
- Mine drainage and remediation techniques
- Minerals Flotation and Separation Techniques
- Corrosion Behavior and Inhibition
- Extraction and Separation Processes
- Radioactive element chemistry and processing
- Mineral Processing and Grinding
- Mining and Resource Management
- Microbial Fuel Cells and Bioremediation
- Microbial Community Ecology and Physiology
- Chromium effects and bioremediation
- Geochemistry and Elemental Analysis
- Coal and Its By-products
- Genomics and Phylogenetic Studies
- Soil Carbon and Nitrogen Dynamics
- Arsenic contamination and mitigation
- Analytical Chemistry and Chromatography
- Tailings Management and Properties
- Biofuel production and bioconversion
- Methane Hydrates and Related Phenomena
- Eicosanoids and Hypertension Pharmacology
- Metalloenzymes and iron-sulfur proteins
- Anaerobic Digestion and Biogas Production
- Enzyme Production and Characterization
- Pesticide and Herbicide Environmental Studies
Bangor University
2015-2024
Dominica State College
2022-2023
Coventry University
2020-2022
Natural History Museum
2010-2022
San Sebastián University
2020
Fundación Ciencia and Vida
2016-2020
Northwestern University
2018
Universidad Andrés Bello
2016
Bigelow Laboratory for Ocean Sciences
2012
Stanford University
2012
Microbial life in extremely low pH (<3) natural and man-made environments may be considerably diverse. Prokaryotic acidophiles (eubacteria archaea) have been the focus of much research activity this area, primarily because importance these microorganisms biotechnology (predominantly commercial biological processing metal ores) environmental pollution (genesis 'acid mine drainage'); however, obligately acidophilic eukaryotes (fungi, yeasts, algae protozoa) are also known, form stable...
REVIEW article Front. Microbiol., 16 March 2012Sec. Microbiological Chemistry and Geomicrobiology https://doi.org/10.3389/fmicb.2012.00096
Waste materials from metal mining, such as mineral tailings, often contain significant amounts of potentially valuable metals particularly where, in many historic operations, the efficiency flotation technologies used to concentrate target minerals was not good those currently available. A two-stage leaching and recovery protocol developed extract copper tailings generated waste two mines operating Spain Serbia. The most effective extraction (84 >90%) achieved by bioleaching at 45 °C, using...
Abstract Genomic sequencing is essential to track the evolution and spread of SARS-CoV-2, optimize molecular tests, treatments, vaccines, guide public health responses. To investigate global SARS-CoV-2 genomic surveillance, we used sequences shared via GISAID estimate impact intensity turnaround times on variant detection in 189 countries. In first two years pandemic, 78% high-income countries sequenced >0.5% their COVID-19 cases, while 42% low- middle-income reached that mark. Around 25%...
ABSTRACT Microorganisms were enumerated and isolated on selective solid media from a pilot-scale stirred-tank bioleaching operation in which polymetallic sulfide concentrate was subjected to biologically accelerated oxidation at 45°C. Four distinct prokaryotes isolated: three bacteria (an Acidithiobacillus caldus -like organism, thermophilic Leptospirillum sp., Sulfobacillus sp.) one archaeon (a Ferroplasma isolate). The relative numbers of these changed the reactors sampled, isolate became...
ABSTRACT The microbial composition of acid streamers (macroscopic biofilms) in acidic, metal-rich waters two locations (an abandoned copper mine and a chalybeate spa) north Wales was studied using cultivation-based biomolecular techniques. Known chemolithotrophic heterotrophic acidophiles were readily isolated from disrupted streamers, but they accounted for only <1 to 7% the total microorganisms present. Fluorescent situ hybridization (FISH) revealed that 80 90% microbes both types β...
Five moderately thermophilic iron-oxidizing bacteria, including representative strains of the three classified species (Sulfobacillus thermosulfidooxidans, Sulfobacillus acidophilus, and Acidimicrobium ferrooxidans), were shown to be capable reducing ferric iron ferrous when they grown under oxygen limitation conditions. Iron reduction was most readily observed isolates as mixotrophs or heterotrophs with glycerol an electron donor; in addition, some able couple oxidation tetrathionate iron....
Summary An extremely acidic (pH 2.5–2.75) metal‐rich stream draining an abandoned mine in the Iberian Pyrite Belt, Spain, was ramified with stratified macroscopic gelatinous microbial growths (‘acid streamers’ or ‘mats’). Microbial communities of streamer/mat sampled at different depths, as well those present water itself, were analysed using a combined biomolecular and cultivation‐based approach. The oxygen‐depleted dominated by chemolithotrophic facultative anaerobe Acidithiobacillus...
Two novel extremely acidophilic, iron-oxidizing actinobacteria were isolated, one from a mine site in North Wales, UK (isolate T23T), and the other geothermal Yellowstone National Park, Wyoming, USA (Y005T). These new belong to subclass Acidimicrobidae, contrast only classified member of (Acidimicrobium ferrooxidans), both isolates obligate heterotrophs. The isolate was mesophilic grew as small rods, while moderate thermophile long filaments, forming macroscopic flocs liquid media. Both...
Nineteen characterized strains and isolates of acidophilic heterotrophic bacteria were screened for their abilities to catalyse the reductive dissolution ferric iron mineral schwertmannite, under oxygen-limiting conditions. Acidocella facilis, Acidobacterium capsulatum, all Acidiphilium, Acidobacterium-like that grew in liquid cultures able reduce iron. In contrast, neither Acidisphaera rubrifaciens nor three Acidisphaera-like tested found have capacity dissimilatory reduction. One two...
Two continuous-flow bench-scale bioreactor systems populated by mixed communities of acidophilic sulfate-reducing bacteria were constructed and tested for their abilities to promote the selective precipitation transition metals (as sulfides) present in synthetic mine waters, using glycerol as electron donor. The objective with first system (selective copper from acidic water containing a variety soluble metals) was achieved maintaining pH ≈ 2.2-2.5. second fed (pH 2.5) 3 mM both zinc ferrous...
Gene transcription (microarrays) and protein levels (proteomics) were compared in cultures of the acidophilic chemolithotroph Acidithiobacillus ferrooxidans grown on elemental sulfur as electron donor under aerobic anaerobic conditions, using either molecular oxygen or ferric iron acceptor, respectively. No evidence supporting role tetrathionate hydrolase arsenic reductase mediating transfer electrons to (as suggested by previous studies) was obtained. In addition, no novel identified....