A5071755895- Landslides and related hazards
- RNA and protein synthesis mechanisms
- Fungal and yeast genetics research
- Tuberculosis Research and Epidemiology
- Geological and Geophysical Studies Worldwide
- Bacteriophages and microbial interactions
- RNA modifications and cancer
- Cryospheric studies and observations
- Plant Disease Resistance and Genetics
- Flood Risk Assessment and Management
- Hepatitis B Virus Studies
- Geology and Paleoclimatology Research
- Archaeological and Historical Studies
- Transgenic Plants and Applications
- Viral Infectious Diseases and Gene Expression in Insects
- Soil erosion and sediment transport
- Plant Virus Research Studies
- Viral Infections and Vectors
- Hepatitis C virus research
- Plant and Fungal Interactions Research
- Aquaculture disease management and microbiota
- Viral Infections and Outbreaks Research
- Medieval Architecture and Archaeology
- Monoclonal and Polyclonal Antibodies Research
- Genomics and Phylogenetic Studies
Universidad de León
2024
Universidad de Cantabria
2020-2023
Empresarios Agrupados
2021
Universidad de La Frontera
2021
Universidad de Oviedo
2015-2020
Pontificia Universidad Católica de Chile
1987-2019
Fundación Ciencia and Vida
2005-2017
Universidad Andrés Bello
2005-2017
Universidad de Los Andes
2014
Millennium Institute for Integrative Biology
2002-2008
Saccharomyces cerevisiae cells were transformed with plasmids containing hybrid genes in which the sequence encoding mature human epidermal growth factor was joined to sequences leader region (preprosegment) of precursor yeast mating pheromone alpha-factor. These accurately process protein and efficiently secrete authentic biologically active into medium.
Epidermal regeneration depends on mitosis and migration of keratinocytes. growth factor is known to stimulate keratinocytes in vitro, thus it might be expected promote wound healing. The results this study show that topical application biosynthetic human epidermal accelerates split-thickness wounds partial-thickness burns. significant enhancement suggests the potential for clinical use accelerating healing burns, from trauma, diabetic ulcers, skin graft donor sites, others.
Copper resistance in yeast is controlled by the CUP1 locus. The level of proportional to copy number this locus, which can be found up 15 tandemly iterated copies. To elucidate molecular mechanisms controlling amplification and expression CUP1, we determined its full nucleotide sequence. We have also identified mapped two transcription units within basic unit laboratory strains. One those inducible copper encodes a low weight binding protein--copper chelatin. increased production chelatin,...
Sixteen bacterial clones containing sequences complementary to yeast PhetRNA were isolated from a collection of hybrid plasmids BamHI restriction endonuclease-generated DNA fragments inserted in the plasmid vector pBR315. Ten these contained with distinct fragments. The sequence Phe-tRNA structural genes and adjacent regions three is reported here. In region flanking tRNA gene, two cloned DNAs similar; third varies considerably. All are bordered by A,T-rich regions. particular, near coding...
The trkB family of transmembrane proteins serves as receptors for BDNF and NT-4/5. is composed a tyrosine kinase-containing isoform well several alternatively spliced “truncated receptors” with identical extracellular ligand-binding domains but very small intracellular domains. two best-characterized truncated receptors, designated trkB.T1 trkB.T2, contain only 23 21 amino acids, respectively. Although it known that the kinase (trkB.FL) capable initiating NT-4/5-induced signal transduction,...
The organization of the ribosomal DNA repeating unit from Saccharomyces cerevisiae has been analyzed. A cloned mapped with restriction enzymes Xma 1, Kpn HindIII, Xba Bgl I + II, and EcoRI. locations sequences which code for 5 S, 5.8 18 25 S RNAs have determined by hybridization purified RNA species endonuclease generated fragments unit. position within repeat was also established sequencing codes 83 nucleotides at 5' end RNA. polarity 35 precursor a combination analysis sequence...
A cDNA clone encoding part of chymotrypsin B was isolated from a library prepared rat pancreatic mRNA and used as probe to isolate the gene. The nucleotide sequence this gene is presented. 4709-base pair transcribed portion inferred sequence, 5' border determined by primer extension on polyadenylated RNA. coding interrupted six introns. active site residues His 57, Asp 102, Ser 195 are encoded separate exons. Moreover, two regions enzyme which form substrate-binding pocket also Thus,...
Three recombinant homodimeric isoforms of platelet-derived growth factor (PDGF) were produced and purified in milligram quantities by expression PDGF A-and B-chains yeast cells. Structural analysis the short long variants PDGF-AA (PDGF-AAS PDGF-AAL) PDGF-BB showed that they had been properly processed assembled into dimers. PDGF-AAs PDGF-AAL found to bind only A-type receptor on human fibroblasts, with affinities 0.1 0.2 uM, respectively. bound cells B-type receptors 0.6 nM both cases. Each...
We have chemically synthesized and expressed in yeast a gene coding for human epidermal growth factor (urogastrone), 53-amino-acid polypeptide that has been shown to promote epithelial cell proliferation inhibit gastric acid secretion. The synthetic gene, consisting of 170 base pairs, was designed with yeast-preferred codons assembled by enzymatic ligation fragments produced phosphoramidite chemistry. DNA synthesis protocol used allows facile oligonucleotides larger than 50 bases. Yeast...
We have constructed two simian virus 40 early replacement recombinants that the coding sequences for hepatitis B surface antigen (HBsAg). One construction, LSV-HBsAg, has region HBsAg but not portion encoding putative pre-surface leader. Transformed monkey kidney cells (COS) infected with this recombinant express large quantities of characteristic partially glycosylated molecule, which are assembled into 22-nm particles appear similar to those produced by human liver virus. This result...
Human coagulation factor VIII:C has been purified approximately 5000-fold from commercial preparations with an average activity yield of 35%. Proteins 92 kD and 77–80 enriched during purification are precipitated by a human serum polyclonal antibody which inhibits activity. Evidence suggests that these polypeptides linked calcium ion bridge. Partial amino acid sequence information proteins obtained the intact products digestion thrombin, endoproteinase lysC, or trypsin after citraconylation....
A DNA fragment containing the structural gene for 5 S ribosomal RNA and intergenic regions before after 35 precursor of Saccharomyces cerevisiae has been amplified in a bacterial plasmid physically mapped by restriction endonuclease cleavage hybridization to purified yeast RNA. The nucleotide sequence fragments carrying adjacent determined. unambiguously identifies gene, determines its polarity within repeating unit, reveals structure promoter termination regions. Partial near beginning end...
Homogeneous RNA polymerase III (RNA nucleotidyltransferase III) has been obtained from yeast. The subunit composition of the enzyme was examined by polyacrylamide gel electrophoresis in presence sodium dodecyl sulfate. is composed 12 putative subunits with molecular weights 160,000, 128,000, 82,000, 41,000, 40,500, 37,000, 34,000, 28,000, 24,000, 20,000, 14,500, and 11,000. high-molecular-weight several smaller yeast are clearly different those enzymes I II, indicating a distinct structure....
Yeast DNA-dependent RNA polymerases I, II, and III are phosphorylated in vivo. cells were grown continuously 32Pi the isolated by a new procedure which allows simultaneous purification of these enzymes from small quantities (35 to 60 g) cells. Each was phosphorylated. The following polymerase polypeptides identified: I subunits 185,000, 44,000, 36,000, 24,000, 20,000 daltons; II subunit 24,000 daltons. incorporated 32P acid-stable but base-labile. Phosphoserine phosphothreonine identified...
To gain insights into coordinated lineage-specification and morphogenetic processes during early embryogenesis, here we report a systematic identification of transcriptional programs mediated by key developmental regulator--Brachyury. High-resolution chromosomal localization mapping Brachyury ChIP sequencing ChIP-exonuclease revealed distinct sequence signatures enriched in Brachyury-bound enhancers. A combination genome-wide vitro vivo perturbation analysis cross-species evolutionary...
ABSTRACT Botrytis cinerea causes gray mold on a great number of host plants. Infection is initiated by airborne conidia that invade the tissue, often penetration intact epidermal cells. To mimic surface properties natural plant surfaces, were incubated apple wax-coated resulting in rapid germination and appressorium formation. Global changes gene expression analyzed microarray hybridization between for 0 h (dormant), 1 (pregermination), 2.5 (postgermination), 4 (appressoria), 15 (early...