A5016384926- Genomics and Chromatin Dynamics
- Epigenetics and DNA Methylation
- RNA Research and Splicing
- RNA modifications and cancer
- Cancer-related gene regulation
- RNA and protein synthesis mechanisms
- DNA Repair Mechanisms
- Insect and Arachnid Ecology and Behavior
- DNA and Nucleic Acid Chemistry
- Neurobiology and Insect Physiology Research
- Genetics and Neurodevelopmental Disorders
- CRISPR and Genetic Engineering
- Histone Deacetylase Inhibitors Research
- Ubiquitin and proteasome pathways
- Virus-based gene therapy research
- Protein Degradation and Inhibitors
- Plant and animal studies
- Viral Infectious Diseases and Gene Expression in Insects
- Sirtuins and Resveratrol in Medicine
- Plant Molecular Biology Research
- RNA Interference and Gene Delivery
- Chromatin Remodeling and Cancer
- Cancer-related Molecular Pathways
- Bacterial Genetics and Biotechnology
- Fungal and yeast genetics research
University of Miami
2023-2025
Howard Hughes Medical Institute
2015-2024
New York University
2015-2024
Sylvester Comprehensive Cancer Center
2023-2024
NYU Langone Health
2014-2024
Dr. John T. Macdonald Foundation
2024
Institut Curie
2018
Cancer Institute (WIA)
2011
The Wistar Institute
1987-2007
Institució Catalana de Recerca i Estudis Avançats
2007
Enhancer of Zeste [E(z)] is a Polycomb-group transcriptional repressor and one the founding members family SET domain-containing proteins. Several SET-domain proteins possess intrinsic histone methyltransferase (HMT) activity. However, recombinant E(z) protein was found to be inactive in HMT assay. Here we report isolation multiprotein complex that contains extra sex combs, suppressor zeste-12 [Su(z)12], binding RbAp46/RbAp48. This complex, which termed Polycomb repressive (PRC) 2, possesses...
Histone lysine methylation is a central modification to mark functionally distinct chromatin regions. In particular, H3-K9 trimethylation has emerged as hallmark of pericentric heterochromatin in mammals. Here we show that H4-K20 also focally enriched at heterochromatin. Intriguingly, by the Suv39h HMTases required for induction trimethylation, although H4 Lys 20 position not an intrinsic substrate these enzymes. By using candidate approach, identified Suv4-20h1 and Suv4-20h2 two novel SET...
Yi Zhang, Huck-Hui Ng, Hediye Erdjument-Bromage, Paul Tempst, Adrian Bird, and Danny Reinberg Howard Hughes Medical Institute (HHMI), Division of Nucleic Acids Enzymology, Department Biochemistry, University Medicine Dentistry New Jersey, Robert Wood Johnson School, Piscataway, Jersey 08854 USA; Cell Molecular Biology, Edinburgh, Edinburgh EH9 3JR, UK; Biology Program, Memorial Sloan Kettering Cancer Center, York, York 10021 USA
The FACT (facilitates chromatin transcription) complex is required for transcript elongation through nucleosomes by RNA polymerase II (Pol II) in vitro. Here, we show that facilitates Pol II-driven transcription destabilizing nucleosomal structure so one histone H2A-H2B dimer removed during enzyme passage. We also demonstrate possesses intrinsic chaperone activity and can deposit core histones onto DNA. Importantly, requires both of its constituent subunits dependent on the highly acidic C...
The initiation of X-chromosome inactivation is thought to be tightly correlated with early differentiation events during mouse development. Here, we show that although initially active, the paternal X chromosome undergoes imprinted from cleavage stages, well before cellular differentiation. A reversal inactive state, a loss epigenetic marks such as histone modifications and polycomb proteins, subsequently occurs in cells inner cell mass (ICM), which give rise embryo-proper random known...
Methylation of histone H3 lysine 27 (H3K27) is a posttranslational modification that highly correlated with genomic silencing. Here we show human UTX, member the Jumonji C family proteins, di- and trimethyl H3K27 demethylase. UTX occupies promoters HOX gene clusters regulates their transcriptional output by modulating recruitment polycomb repressive complex 1 monoubiquitination H2A. Moreover, associates mixed-lineage leukemia (MLL) 2/3 complexes, during retinoic acid signaling events, to...