A5045293390- Pregnancy and preeclampsia studies
- Sphingolipid Metabolism and Signaling
- Reproductive System and Pregnancy
- Endoplasmic Reticulum Stress and Disease
- FOXO transcription factor regulation
- Birth, Development, and Health
- Gestational Diabetes Research and Management
- Peroxisome Proliferator-Activated Receptors
- Folate and B Vitamins Research
- Growth Hormone and Insulin-like Growth Factors
- Heat shock proteins research
- Lipid metabolism and biosynthesis
- Lipid Membrane Structure and Behavior
- Hepatitis C virus research
- Aldose Reductase and Taurine
- Lipid metabolism and disorders
- Herpesvirus Infections and Treatments
- Genetics and Neurodevelopmental Disorders
- HIV Research and Treatment
- ATP Synthase and ATPases Research
- Liver physiology and pathology
- Extracellular vesicles in disease
- Cancer Risks and Factors
- Disaster Response and Management
- Medicinal Plants and Bioactive Compounds
Universidad de Los Andes, Chile
2013-2017
University of Kentucky
2006-2016
Johannes Gutenberg University Mainz
2001
ABSTRACT As an initial approach to studying the molecular replication mechanisms of hepatitis C virus (HCV), a major causative agent acute and chronic liver disease, we have recently developed selectable self-replicating RNAs. These replicons lacked region encoding structural proteins instead carried gene neomycin phosphotransferase. Although levels these RNAs within selected cells were high, number G418-resistant colonies was reproducibly low. In search for reason, performed detailed...
Studies completed to date provide persuasive evidence that placental cell-derived exosomes play a significant role in intercellular communication pathways potentially contribute placentation and development of materno-fetal vascular circulation. The aim this study was establish the gestational-age release profile bioactivity exosome maternal plasma. Plasma samples (n = 20 per pregnant group) were obtained from non-pregnant women first (FT, 6–12 weeks), second (ST, 22–24 weeks) third (TT,...
Introduction Preeclampsia is a maternal hypertensive disorder with uncertain etiology and leading cause of fetal mortality worldwide, causing nearly 40% premature births delivered before 35 weeks gestation. The first stage preeclampsia characterized by reduction utero-placental blood flow which reflected in high pressure proteinuria during the second half pregnancy. In human placenta androgens derived from adrenal glands are converted into estrogens enzymatic action placental aromatase. This...
The Glypican 3 (Gpc3) gene is expressed abundantly in the fetal liver, inactive normal adult and frequently reactivated hepatocellular carcinoma (HCC). This reactivation HCC has led to considerable interest Gpc3 as a diagnostic tumor marker its possible role tumorigenesis. Despite this interest, basis for regulation poorly understood. On of similarities between alpha-fetoprotein expression we reasoned that common factors might regulate these 2 genes. Here identify zinc fingers homeoboxes...
Introduction Preeclampsia (PE) is a gestational disorder, manifested in the second half of pregnancy by maternal hypertension, proteinuria and generalized edema. PE major cause fetal morbidity mortality, accounting for nearly 40% all premature births worldwide. Bioactive sphingolipids are emerging as key molecules involved etiopathogenesis PE, characterized angiogenic imbalance symptoms metabolic syndrome. The aim this study was to compare cross-gestational profile circulating bioactive...
Objective . To evaluate the role of key enzymes in methionine-homocysteine metabolism (MHM) physiopathology preeclampsia (PE). Methods Plasma and placenta from pregnant women (32 controls 16 PE patients) were analyzed after informed consent. Protein was quantified by western blot. RNA obtained with purification kit reverse transcritase followed real-time PCR (RT-qPCR). Identification C677T A1298C methylenetetrahydrofolate reductase (MTHFR) single-nucleotide polymorphisms (SNPs) A2756G...
Abstract The process of aging has recently been shown to substantially affect the ability cells respond inflammatory challenges. We demonstrate that leads hepatic hyperresponsiveness interleukin 1β (IL-1β), and we examine factors could be responsible for this phenomenon. IL-1β-induced phosphorylation c-jun N-terminal kinase (JNK) in hepatocytes isolated from aged rats was 3 times more potent than young rats. Moreover, JNK activated by lower doses IL-1β. These age-related changes correlated...
During gestation, low oxygen environment is a major determinant of early placentation process, while persistent placental hypoxia leads to pregnancy-related complications such as preeclampsia (PE) and intrauterine growth restriction (IUGR). PE affects 5%-8% all pregnancies worldwide cause maternal fetal morbidity mortality. development, due poor trophoblast invasion reduced uteroplacental perfusion endothelial dysfunction clinical manifestation PE. Here we hypothesized that nuclear factor...
Preeclampsia (PE), is a serious pregnancy disorder characterized in the early gestation by shallow trophoblast invasion, impaired placental neo-angiogenesis, hypoxia and ischemia, which leads to maternal fetal morbidity mortality. Here we hypothesized that angiogenic sphingosine kinase-1 (SPHK1)/sphingosine-1-phosphate (S1P) receptors pathway PE. We found SPHK1 mRNA protein expression are down-regulated term placentae chorionic villous explants from patients with PE or severe (PES), compared...
The level of insulin-like growth factor-binding protein 1 (IGFBP1), a liver-produced serum that regulates factor-I bioactivity, glucose homeostasis, and tissue regeneration, increases during inflammation. This manuscript describes novel pathway for the regulation hepatic IGFBP1 mRNA levels by interleukin (IL)-1β. Experiments with luciferase reporter system show IL-1β stimulates transcriptional activity from 1-kb promoter region IGFBP1. Although stimulation suppresses insulin activation...
The objective of this study is to understand the role Neutral sphingomyelinase‐2 (NSMase‐2) in hepatic response Interleukin 1β (IL‐1β). IL‐1β signaling cascade initiated by rapid phosphorylation receptor activated kinase‐1 (IRAK‐1) ligand binding. Subsequently, phoshporylated IRAK‐1 ubiquitinated and degraded rate its degradation known affect magnitude response. We show that activity protein phosphatase 2A (PP2A) gradually increases stimulation with a maximum effect seen 30 minutes after...
Background: Natural disasters increase the level population stress, including pregnant women, who can experience prenatal maternal affecting to fetus and trigger perinatal complications, such as low birth weight, smaller head circumference, etc. However, little is known about effects of earthquake on outcomes. Objective: To evaluate effect occurred February 27, 2010 outcomes Chilean examine these by timing exposure during pregnancy newborn gender. Methods: A register-based study was...
Human hepatoma cells (HepG2) synthesize and secrete several plasma proteins that are inhibited in a time- dose-dependent manner after vaccinia virus infection. However, infection of the HepG2 with low dose (up to 1 plaque forming unit/cell) stimulated expression alpha-1-antichymotrypsin, which was demonstrated by means electroimmunoassay Northern blot analysis. This stimulation appeared be on level transcription as shown transient transfection experiments using various...