A5069080804- Extracellular vesicles in disease
- Pregnancy and preeclampsia studies
- MicroRNA in disease regulation
- Gestational Diabetes Research and Management
- Reproductive System and Pregnancy
- Birth, Development, and Health
- Circular RNAs in diseases
- Preterm Birth and Chorioamnionitis
- Advanced biosensing and bioanalysis techniques
- Cardiovascular Issues in Pregnancy
- Cancer-related molecular mechanisms research
- RNA Interference and Gene Delivery
- Maternal and fetal healthcare
- RNA modifications and cancer
- Adenosine and Purinergic Signaling
- Mesenchymal stem cell research
- Pregnancy and Medication Impact
- Pancreatic function and diabetes
- Cardiovascular Disease and Adiposity
- Advanced Nanomaterials in Catalysis
- RNA Research and Splicing
- Neonatal Respiratory Health Research
- Renal and related cancers
- Oral microbiology and periodontitis research
- Nanoplatforms for cancer theranostics
Royal Brisbane and Women's Hospital
2016-2025
The University of Queensland
2016-2025
Ochsner Medical Center
2015-2024
Hospital Universitario Son Espases
2024
Universidad Pedro de Valdivia
2021-2024
University of Concepción
2010-2023
Universidad de Santiago de Chile
2021-2023
Ochsner Baptist Medical Center
2016-2020
Translational Research Institute
2017-2020
Mater Research
2017-2020
Human placenta releases specific nanovesicles (i.e. exosomes) into the maternal circulation during pregnancy, however, presence of placenta-derived exosomes in blood early pregnancy remains to be established. The aim this study was characterise gestational age related changes concentration first trimester from 6 12 weeks) plasma women with normal pregnancies. A time-series experimental design used establish pregnancy-associated exosome concentrations trimester. series were collected healthy...
Studies completed to date provide persuasive evidence that placental cell-derived exosomes play a significant role in intercellular communication pathways potentially contribute placentation and development of materno-fetal vascular circulation. The aim this study was establish the gestational-age release profile bioactivity exosome maternal plasma. Plasma samples (n = 20 per pregnant group) were obtained from non-pregnant women first (FT, 6–12 weeks), second (ST, 22–24 weeks) third (TT,...
Vasculogenesis and angiogenesis are critical processes in fetal circulation placental vasculature development. Placental mesenchymal stem cells (pMSC) known to release paracrine factors (some of which contained within exosomes) that promote cell migration. The aims this study were: determine the effects oxygen tension on exosomes from pMSC; establish pMSC-derived migration angiogenic tube formation microvascular endothelial (hPMEC). pMSC were isolated villi (8–12 weeks gestation, n = 6)...
Abstract Context There is a need to develop strategies for early prediction of patients who will preeclampsia (PE) establish preventive reduce the prevalence and severity disease their associated complications. Objective The objective this study was investigate whether exosomes microRNA cargo present in maternal circulation can be used as biomarker PE. Design, Setting, Patients, Interventions A retrospective stratified design quantify total placenta-derived plasma normal (n = 32 per time...
Although there is significant interest in elucidating the role of placenta-derived exosomes (PdEs) during pregnancy, exosomal profile pregnancies complicated by gestational diabetes mellitus (GDM) remains to be established. The aim this study was compare gestational-age PdEs maternal plasma GDM with normal and determine effect on cytokine release from human umbilical vein endothelial cells. A prospective cohort patients sampled at three time points pregnancy for each patient (i.e., 11–14,...
Most of the current exosome-analysis strategies are time-consuming and largely dependent on commercial extraction kit-based preisolation step, which requires extensive sample manipulations, costly isolation kits, reagents, tedious procedures, sophisticated equipment is prone to bias/artifacts. Herein we introduce a simple method for direct subsequent detection specific population exosomes using an engineered superparamagnetic material with multifunctional properties, namely, gold-loaded...
Abstract Background The role of exosomes in the pathogenesis and metastatic spread cancer remains to be fully elucidated. Recent studies support hypothesis that release from cells modifies local extracellular conditions promote cell growth neovascularisation. In addition, may modify phenotype parent and/or target cell. For example, sequestration signaling mediators into reduce their intracellular bioavailability thereby altering potential. fusion released with delivery also function...
Abstract Silica materials are used in a wide range of applications such as catalysis, photocatalysis, CO2 capture, and environmental remediation. These nanomaterials (NMs) have been extensively investigated since the advent Stöber silica. However, absence pores small surface area silica limits its applications. Later, discovery MCM-41 type mesoporous using surfactants structural directing agents became revolutionary field NMs. This review focuses on methods for synthesizing (NMs), especially...
Small extracellular vesicles (sEVs) provide major promise for advances in cancer diagnostics, prognostics, and therapeutics, ascribed to their distinctive cargo reflective of pathophysiological status, active involvement intercellular communication, as well ubiquity stability bodily fluids. As a result, the field sEV research has expanded exponentially. Nevertheless, there is lack standardisation methods isolation from cells grown serum-containing media. The majority researchers use media...
Abstract During the last decade, there has been great interest in elucidating biological role of extracellular vesicles (EVs), particularly, their hormone-like cell-to-cell communication. The field endocrinology is uniquely placed to provide insight into functions EVs, which are secreted from all cells fluids and carry endocrine signals engage paracellular distal interactions. EVs a heterogeneous population membrane-bound varying size, content, bioactivity. specifically packaged with...
Extracellular vesicles (EVs) occur in a variety of bodily fluids and have gained recent attraction as natural materials due to their bioactive surfaces, internal cargo, role intercellular communication. EVs contain various biomolecules, including surface cytoplasmic proteins; nucleic acids that are often representative the originating cells. can transfer content other cells, process is thought be important for several biological processes, immune responses, oncogenesis, angiogenesis. An...
Purpose Robust biomarkers that predict disease outcomes amongst COVID-19 patients are necessary for both patient triage and resource prioritisation. Numerous candidate have been proposed COVID-19. However, at present, there is no consensus on the best diagnostic approach to in infected patients. Moreover, it not clear whether such tools would apply other potentially pandemic pathogens therefore of use as stockpile future preparedness. Methods We conducted a multi-cohort observational study...
Bacterial extracellular vesicles (BEVs) are naturally occurring bioactive membrane-bound nanoparticles released by both gram-negative and gram-positive bacterial species, exhibiting a multifaceted role in mediating host-microbe interactions across various physiological conditions. Increasing evidence supports BEVs as essential mediators of cell-to-cell communicaiton, influencing pathogenicity, disease mechanisms, modulating the host immune response. However, extent to which these...
Migration of extravillous trophoblasts (EVT) into decidua and myometrium is a critical process in the conversion maternal spiral arterioles establishing placenta perfusion. EVT migration affected by cell-to-cell communication oxygen tension. While release exosomes from placental cells has been identified as significant pathway materno-fetal communication, role placental-derived placentation yet to be established. The aim this study was establish effect tension on bioactivity cytotrophoblast...
Context: Hyperglycemia and hypoxia are risk factors of metabolic complication during pregnancy. The interactions between oxygen glucose-sensing pathways that regulate exosome bioactivity from placental cells, however, have not been established. Objective: aim this study was to test the hypothesis exosomal signaling by cells (defined as number exosomes released per unit time their bioactivity) is responsive extracellular glucose concentration. Methods: First-trimester primary trophoblast were...
The enzyme-mimicking activity of iron oxide based nanostructures has provided a significant advantage in developing advanced molecular sensors for biomedical and environmental applications. Herein, we introduce the horseradish peroxidase (HRP)-like gold-loaded nanoporous ferric nanocubes (Au–NPFe2O3NC) development sensor with enhanced electrocatalytic colorimetric (naked eye) detection autoantibodies. results showed that Au–NPFe2O3NC exhibits peroxidase-like toward catalytic oxidation...
Vascular smooth muscle cells (VSMCs) migration is a critical process during human uterine spiral artery (SpA) remodeling and successful pregnancy. Extravillous trophoblast (EVT) interact with VSMC enhance their migration, however, the mechanisms by which EVT remodel SpA remain to be fully elucidated. We hypothesize that exosomes released from promote migration.JEG-3 HTR-8/SVneo cell lines were used as models for EVT. Cells cultured at 37°C humidified under an atmosphere of 5% CO2-balanced N2...
Our objective was to determine the amniotic fluid-derived exosomal proteomic profile in patients who had spontaneous preterm birth (PTB) or premature rupture of membranes (pPROM) compared with those delivered at term. A cross-sectional study a retrospective cohort used quantify and protein content exosomes present fluid, PTB pPROM, normal term labor (TL) not (TNIL) pregnancies. Exosomes were isolated by differential centrifugation quantified using nanocrystals (Qdot) coupled CD63 placental...
Despite decades of research in the field human reproduction, mechanisms responsible for parturition still remain elusive. The objective this study was to describe changes exosomal miRNA concentrations circulating maternal plasma between mothers delivering term and preterm neonates, across gestation using a longitudinal design. This descriptive identifies content exosomes present birth (PTB) (n = 20 n 10 per each gestational period, respectively) (i.e., first, second, third trimesters at time...