A5001755660- Stellar, planetary, and galactic studies
- Astronomy and Astrophysical Research
- Galaxies: Formation, Evolution, Phenomena
- Advanced Proteomics Techniques and Applications
- Mass Spectrometry Techniques and Applications
- Astrophysics and Star Formation Studies
- RNA and protein synthesis mechanisms
- Metabolomics and Mass Spectrometry Studies
- Pregnancy and preeclampsia studies
- Glycosylation and Glycoproteins Research
- Cosmology and Gravitation Theories
- Extracellular vesicles in disease
- Monoclonal and Polyclonal Antibodies Research
- Material Science and Thermodynamics
- Helminth infection and control
- Cellular transport and secretion
- Protein purification and stability
- Coccidia and coccidiosis research
- Impact of Light on Environment and Health
- Peptidase Inhibition and Analysis
- Mesenchymal stem cell research
- Machine Learning in Bioinformatics
- Analytical Chemistry and Chromatography
- Bacteriophages and microbial interactions
- Astronomical Observations and Instrumentation
TissuPath
2024
The University of Sydney
2023-2024
Children's Medical Research Institute
2024
The University of Queensland
2012-2016
Royal Brisbane and Women's Hospital
2014-2016
Spanish National Cancer Research Centre
2009-2014
Centro Nacional de Epidemiología
2011-2012
Centro de Investigación del Cáncer
2010-2012
University of Missouri–Kansas City
2005-2010
Centro Nacional de Investigaciones Científicas
2009
Bacterial pathogenicity islands (PAI) often encode both effector molecules responsible for disease and secretion systems that deliver these effectors to host cells. Human enterohemorrhagic Escherichia coli (EHEC), enteropathogenic E. , the mouse pathogen Citrobacter rodentium (CR) possess locus of enterocyte effacement (LEE) PAI. We systematically mutagenized all 41 CR LEE genes functionally characterized mutants in vitro a murine infection model. identified 33 virulence factors, including...
Signal peptide peptidase (SPP) catalyzes intramembrane proteolysis of some signal peptides after they have been cleaved from a preprotein. In humans, SPP activity is required to generate sequence–derived human lymphocyte antigen–E epitopes that are recognized by the immune system, and process hepatitis C virus core protein. We identified as polytopic membrane protein with sequence motifs characteristic presenilin-type aspartic proteases. potential eukaryotic homologs may represent another...
view Abstract Citations (629) References (53) Co-Reads Similar Papers Volume Content Graphics Metrics Export Citation NASA/ADS The Formation of Globular Clusters in Merging and Interacting Galaxies Ashman, Keith M. ; Zepf, Stephen E. We suggest that at least some globular clusters are formed during the interaction or merger galaxies. Such events could explain disk population Galaxy, young globulars Magellanic Clouds, excess around ellipticals relative to spirals same luminosity anomalously...
Vasculogenesis and angiogenesis are critical processes in fetal circulation placental vasculature development. Placental mesenchymal stem cells (pMSC) known to release paracrine factors (some of which contained within exosomes) that promote cell migration. The aims this study were: determine the effects oxygen tension on exosomes from pMSC; establish pMSC-derived migration angiogenic tube formation microvascular endothelial (hPMEC). pMSC were isolated villi (8–12 weeks gestation, n = 6)...
A set of 76 open clusters with abundances based upon DDO photometry and/or moderate dispersion spectroscopy has been transformed to a common metallicity scale and used study the local structure evolution galactic disk. The distribution galactocentric distance is best described by two distinct zones. Between R<SUB>GC</SUB>=6.5 10 kpc, mean [Fe/H]=0.0 0.1 dex; there is, at best, weak evidence for shallow abundance gradient over this range. Beyond = between 0.10 0.15 dex, but [Fe/H] -0.3,...
We show that current observations support the hypothesis globular clusters form in galaxy mergers. In a previous paper, we presented model which cluster formation is result of interactions and mergers galaxies. Here, this compared with new systems recent normal elliptical find our consistent number luminosity young currently merging If galaxies through spiral galaxies, also predicts should have at least two peaks metallicity distribution. nearby prediction. More generally, presence more than...
Enterohaemorrhagic Escherichia coli (EHEC) O157:H7 uses a specialized protein translocation apparatus, the type III secretion system (TTSS), to deliver bacterial effector proteins into host cells. These effectors interfere with cytoskeletal pathways and signalling cascades facilitate survival replication promote disease. The genes encoding TTSS all known secreted in EHEC are localized single pathogenicity island on chromosome as locus for enterocyte effacement (LEE). In this study, we...
The combination of stable isotope labeling (SIL) with mass spectrometry (MS) allows comparison the abundance thousands proteins in complex mixtures. However, interpretation large data sets generated by these techniques remains a challenge because appropriate statistical standards are lacking. Here, we present generally applicable model that accurately explains behavior obtained using current SIL approaches, including (18)O, iTRAQ, and SILAC labeling, different MS instruments. decomposes...
Migration of extravillous trophoblasts (EVT) into decidua and myometrium is a critical process in the conversion maternal spiral arterioles establishing placenta perfusion. EVT migration affected by cell-to-cell communication oxygen tension. While release exosomes from placental cells has been identified as significant pathway materno-fetal communication, role placental-derived placentation yet to be established. The aim this study was establish effect tension on bioactivity cytotrophoblast...
Abstract Selected reaction monitoring (SRM) MS is proving to be a popular approach for targeted quantitative proteomics. The use of proteotypic peptides as candidates SRM analysis wise first step in method design. obvious reason this the need avoid redundancy at sequence level, however incidental. true that homologous result mass‐to‐charge domain. This may seem like trivial subtlety, however, we believe an issue far greater significance than proteomic community aware. VIEWPOINT article...
Abstract Reproducible research is the bedrock of experimental science. To enable deployment large-scale proteomics, we assess reproducibility mass spectrometry (MS) over time and across instruments develop computational methods for improving quantitative accuracy. We perform 1560 data independent acquisition (DIA)-MS runs eight samples containing known proportions ovarian prostate cancer tissue yeast, or control HEK293T cells. Replicates are run on six spectrometers operating continuously...
We present images of the recent galaxy merger NGC 3256 obtained with Wide Field Planetary Camera 2 Hubble Space Telescope in B and I filters. show that there is a large population more than 1000 compact, bright, blue objects this within 7 kpc × region studied. These have sizes, colors, luminosities like those expected for young Galactic globular clusters, ages ranging from few to several hundred megayears. On basis, we identify at least some fraction as clusters. The cluster system makes up...
Hydrolysis of triglycerides is central to energy homeostasis in white adipose tissue (WAT). Hormone-sensitive lipase (HSL) was previously felt mediate all lipolysis WAT. Surprisingly, HSL-deficient mice show active HSL-independent lipolysis, suggesting that other lipase(s) also triglyceride hydrolysis. To clarify this, we used functional proteomics detect non-HSL mouse After cell fractionation intraabdominal WAT, most neutral activity localized the 100,000 x g infranatant and fat cake...
To identify the components of conditioned medium obtained from intervertebral disc nucleus pulposus-derived canine notochord cells, and to evaluate capacity such factors affect disc-derived chondrocyte gene expression aggrecan, versican, hyaluronic acid synthase 2 (HAS-2) as a function culture conditions.Canine cells nonchondrodystrophic dogs were cultured within alginate beads under conditions serum deficiency (Dulbecco's modified Eagle's [DMEM]) produce cell-conditioned (NCCM). NCCM was...
We have identified 1057 globular cluster candidates in a WFPC2 image of the inner region M87. The luminosity function (GCLF) can be well fitted by Gaussian profile with mean value m0V=23.67±0.07 mag and σ=1.39±0.06 mag. GCLF five radial bins is found to statistically same at all points, showing no clear evidence dynamical destruction processes based on (LF). Similarly, there obvious trend between half-light radius clusters galactocentric distance. core density distribution Rc=56'',...
Current ideas on the amount, distribution, and nature of dark matter in galaxies are reviewed. Observations indicate that halos around most, if not all, galaxies. Recent evidence suggests many dwarf have higher dark-matter fractions than normal as well central densities. Some spiral galaxy rotation curves rising others falling at optical radius, thereby weakening "disk-halo conspiracy." Observational theoretical techniques to probe ellipticals becoming more refined, various methods being...
Tyrosylprotein sulfotransferase (TPST) is a 54- to 50-kDa integral membrane glycoprotein of the trans-Golgi network found in essentially all tissues investigated, catalyzing tyrosine O-sulfation soluble and proteins passing through this compartment. Here we describe ( i ) an approach identify TPST protein, referred as MSC m odification after s ubstrate c rosslinking) labeling, which based on crosslinking substrate peptide followed by intramolecular [ 35 S]sulfate transfer from cosubstrate...
Advances in high-throughput mass spectrometry are making proteomics an increasingly important tool genome annotation projects. Peptides detected experiments can be used to validate gene models and verify the translation of putative coding sequences (CDSs). Here, we have identified peptides that cover 35% genes annotated by GENCODE consortium for human as part a comprehensive analysis experimental spectra from two large publicly available databases. We protein "novel" "putative"...