A5033256427- Advanced Proteomics Techniques and Applications
- Pancreatitis Pathology and Treatment
- Mass Spectrometry Techniques and Applications
- Neurobiology and Insect Physiology Research
- Insect and Arachnid Ecology and Behavior
- Insect and Pesticide Research
- Venomous Animal Envenomation and Studies
- Tissue Engineering and Regenerative Medicine
- Erythrocyte Function and Pathophysiology
- Electrospun Nanofibers in Biomedical Applications
- Biochemical and Structural Characterization
- Antimicrobial Peptides and Activities
- Amino Acid Enzymes and Metabolism
- Radiomics and Machine Learning in Medical Imaging
- Advanced Biosensing Techniques and Applications
- Advanced biosensing and bioanalysis techniques
- Pancreatic function and diabetes
- Metabolomics and Mass Spectrometry Studies
- Ion channel regulation and function
- Colorectal Cancer Screening and Detection
- Bioinformatics and Genomic Networks
- Cancer Genomics and Diagnostics
- Gastric Cancer Management and Outcomes
- Ferroptosis and cancer prognosis
- Cancer Diagnosis and Treatment
The University of Sydney
2008-2025
Children's Medical Research Institute
2020-2025
University of Southern California
2023-2024
Westmead Institute
2022
University of Technology Sydney
2013-2016
La Jolla Bioengineering Institute
2003
University of California, San Diego
2002-2003
More than 570,000 coronary artery bypass grafts are implanted each year, creating an important demand for small-diameter vascular grafts. For patients who lack adequate internal mammary or saphenous vein, tissue-engineered arteries may prove useful. However, the time needed to tissue engineer (7 weeks more) is too long many patients. Decellularized cadaveric human another possible source of conduit, but limited availability and potential disease transmission limit their widespread use. In...
The proteome provides unique insights into disease biology beyond the genome and transcriptome. A lack of large proteomic datasets has restricted identification new cancer biomarkers. Here, proteomes 949 cell lines across 28 tissue types are analyzed by mass spectrometry. Deploying a workflow to quantify 8,498 proteins, these data capture evidence cell-type post-transcriptional modifications. Integrating multi-omics, drug response, CRISPR-Cas9 gene essentiality screens with deep...
Abstract Reproducible research is the bedrock of experimental science. To enable deployment large-scale proteomics, we assess reproducibility mass spectrometry (MS) over time and across instruments develop computational methods for improving quantitative accuracy. We perform 1560 data independent acquisition (DIA)-MS runs eight samples containing known proportions ovarian prostate cancer tissue yeast, or control HEK293T cells. Replicates are run on six spectrometers operating continuously...
Immature skeletal muscle cells, or myoblasts, have been used in cellular cardiomyoplasty attempts to regenerate cardiac tissue by injection of cells into damaged myocardium. In some studies, within myoblast implant sites may be morphologically similar muscle. We hypothesized that identifiable aspects the milieu contribute growth and development implanted myoblasts vivo. To test this hypothesis, we designed a novel vitro system mimic electrical biochemical environment native This enabled us...
Proteomic analysis by mass spectrometry of small (≤2 mg) solid tissue samples from diverse formats requires high throughput and comprehensive proteome coverage. We developed a nearly universal, rapid, robust protocol for sample preparation, suitable high-throughput projects that encompass most cell or types. This end-to-end workflow extends original to loading the spectrometer is centered on one-tube homogenization digestion method called Heat 'n Beat (HnB). It applicable tissues, regardless...
Abstract Formalin-fixed paraffin-embedded (FFPE) tissues present an important resource for cancer proteomics. They are more readily available than fresh frozen (FF) and can be stored at ambient temperature decades. FFPE blocks largely stable long-term preservation of tumour histology, but the antigenicity some proteins in sections degrades over time resulting deteriorating performance immunohistochemistry (IHC). It is not known whether that have previously been cut from used liquid...
Abstract Introduction: Esophageal adenocarcinoma (EAC) is an aggressive malignancy with poor survival rates. Current management relies on tumor stage and grade, neoadjuvant therapy (NT) showing improved outcomes over surgery alone. However, robust biomarkers are still required to improve staging patient selection for therapy, including NT. Methods: A training cohort of fresh frozen samples from 64 patients EAC, not treated NT, was analyzed by data-independent acquisition mass spectrometry....
The l-to-d-peptide isomerase from the venom of platypus (Ornithorhyncus anatinus) is first such enzyme to be reported for a mammal. In delineating its catalytic mechanism and broader roles in animal, substrate specificity was explored. We used N-terminal segments defensin-like peptides DLP-2 DLP-4 natriuretic peptide OvCNP as substrates. DLP analogues IMFsrs ImFsrs (srs solubilizing chain; lowercase letters denote d-amino acid) were effective substrates isomerase; it appears recognize...
Rationale Compared with other animal venoms, ant venoms remain little explored. Ants have evolved complex to rapidly immobilize arthropod prey and protect their colonies from predators pathogens. Many ants retained peptide‐rich that are similar those of groups. Methods With the goal conducting a broad comprehensive survey venom peptide diversity, we investigated composition 82 stinging species nine subfamilies using matrix‐assisted laser desorption/ionisation time‐of‐flight mass spectrometry...
Animal venom peptides are currently being developed as novel drugs and bioinsecticides. Because ants use venoms for defense predation, venomous represent an untapped source of potential bioactive toxins. This study compared the protein peptide components poneroid Neoponera commutata, apicalis, Odontomachus hastatus formicoid Ectatomma tuberculatum, brunneum, Myrmecia gulosa. 1D 2D PAGE revealed proteins in mass range <10 to >250 kDa. NanoLC-ESI-QTOF MS/MS analysis tryptic presence common...
Abstract Background Disparities in life-saving interventions for low-income patients with cirrhosis necessitate innovative models of care. Aim To implement a novel generalist-led FLuid ASPiration (FLASP) clinic to reduce emergency department (ED) care refractory ascites. Setting A large safety net hospital Los Angeles. Participants MediCal paracentesis the ED from 6/1/2020 1/31/2021 or FLASP 3/1/2021 4/30/2022. Program Description According RE-AIM, adoption obtained administrative...
Abstract Male platypus ( Ornithorhynchus anatinus ) venom has a peptidyl aminoacyl L / D ‐isomerase (hereafter called peptide isomerase) that converts the second amino acid residue in from N‐terminus ‐ to ‐form, and vice versa. A reversed‐phase high‐performance liquid chromatography (RP‐HPLC) assay been developed monitor interconversion using synthetic hexapeptides derived defensin‐like peptide‐2 (DLP‐2) DLP‐4 as substrates. It was hypothesised animals other than would have isomerase with...
RBCs (red blood cells) circulating through narrow capillaries withstand major deformation. The mechanical and chemical stresses commonly exerted on continue to attract interest for the study of membrane structure function. Snake venoms are lethal biochemical 'cocktails' that often contain haemotoxins, metalloproteinases, myotoxins, neurotoxins, phosphodiesterases, phospholipases proteases. We have monitored effects 4 snake (Pseudechis guttatus, Oxyuranus scutellatus, Notechis scutatus Naja...
An anatomical feature of the platypus (Ornithorhynchus anatinus) that is seen in only one other mammal, echidna, male has a crural glandular system produces venom used for defence and territorial–breeding functions; whether echidna similarly venomous not yet established. Platypus contains many novel proteins peptides are different from those reptilian venom. It also causes pain symptoms experienced by any kind envenomation. Five types have been isolated identified venom, namely:...
The monotremes (the echidnas and the platypus) display both mammalian reptilian features. Male have a bilateral crural gland that is connected via duct to spur on each hind limb. appear not use their spurs as weapons in aggressive acts, but system may role reproductive behaviour because it appears only be active during breeding season. secretions produced by echidna’s hitherto been biochemically or pharmacologically characterised. We used reverse-phase high-performance liquid chromatography...
3120 Background: Accurate tumour classification based upon tissue of origin (TOO) remains important to guide treatment selection and prognosis but can be challenging in patients with poorly differentiated malignancy, cancer unknown primary (CUP) or those prior malignancy. Data-independent acquisition mass spectrometry (DIA-MS)-based proteomics is emerging as a potential clinical diagnostic prognostic tool. We aimed develop protein-based signature identify histological subtype adenocarcinoma...
The three‐dimensional structure of a chemically synthesized peptide that we have called ‘intermediate’ defensin‐like (Int‐DLP), from the platypus genome, was determined by nuclear magnetic resonance (NMR) spectroscopy; and its antimicrobial activity investigated. overall structural fold Int‐DLP similar to DLPs β‐defensins, however presence third antiparallel β‐strand makes more β‐defensins than DLPs. displayed potent against Staphylococcus aureus Pseudomonas aeruginosa . four arginine...
Summary The proteome provides unique insights into biology and disease beyond the genome transcriptome. Lack of large proteomic datasets has restricted identification new cancer biomarkers. Here, proteomes 949 cell lines across 28 tissue types were analyzed by mass spectrometry. Deploying a clinically-relevant workflow to quantify 8,498 proteins, these data capture evidence type post-transcriptional modifications. Integrating multi-omics, drug response CRISPR-Cas9 gene essentiality screens...