A5051392151- Extracellular vesicles in disease
- Pregnancy and preeclampsia studies
- MicroRNA in disease regulation
- Gestational Diabetes Research and Management
- Reproductive System and Pregnancy
- Preterm Birth and Chorioamnionitis
- Circular RNAs in diseases
- Cardiovascular Issues in Pregnancy
- Health Systems, Economic Evaluations, Quality of Life
- Pregnancy and Medication Impact
- Neonatal Respiratory Health Research
- Advanced Nanomaterials in Catalysis
- Hyperglycemia and glycemic control in critically ill and hospitalized patients
- Respiratory viral infections research
- SARS-CoV-2 and COVID-19 Research
- Viral Infections and Outbreaks Research
- Ear and Head Tumors
- Pharmaceutical Economics and Policy
- Bartonella species infections research
- Animal health and immunology
- Salivary Gland Disorders and Functions
- Urologic and reproductive health conditions
- Peripheral Artery Disease Management
- SARS-CoV-2 detection and testing
- Dental Research and COVID-19
Royal Brisbane and Women's Hospital
2016-2025
The University of Queensland
2016-2025
University of Concepción
2012-2019
Hospital Regional de Concepción
2014
Pontificia Universidad Católica de Chile
2013
Baptist Hospital
1999
Saint Thomas Midtown Hospital
1999
Mallinckrodt (United States)
1999
St. Luke's Hospital
1999
Washington University Medical Center
1999
Most of the current exosome-analysis strategies are time-consuming and largely dependent on commercial extraction kit-based preisolation step, which requires extensive sample manipulations, costly isolation kits, reagents, tedious procedures, sophisticated equipment is prone to bias/artifacts. Herein we introduce a simple method for direct subsequent detection specific population exosomes using an engineered superparamagnetic material with multifunctional properties, namely, gold-loaded...
Small extracellular vesicles (sEVs) provide major promise for advances in cancer diagnostics, prognostics, and therapeutics, ascribed to their distinctive cargo reflective of pathophysiological status, active involvement intercellular communication, as well ubiquity stability bodily fluids. As a result, the field sEV research has expanded exponentially. Nevertheless, there is lack standardisation methods isolation from cells grown serum-containing media. The majority researchers use media...
Our objective was to determine the amniotic fluid-derived exosomal proteomic profile in patients who had spontaneous preterm birth (PTB) or premature rupture of membranes (pPROM) compared with those delivered at term. A cross-sectional study a retrospective cohort used quantify and protein content exosomes present fluid, PTB pPROM, normal term labor (TL) not (TNIL) pregnancies. Exosomes were isolated by differential centrifugation quantified using nanocrystals (Qdot) coupled CD63 placental...
There is increasing evidence that miRNAs, which are enriched in nanovesicles called exosomes, important regulators of gene expression. When compared with normal pregnancies, pregnancies gestational diabetes mellitus (GDM) associated skeletal muscle insulin resistance as well increased levels circulating placental exosomes. Here we investigated whether exosomes GDM carry a specific set miRNAs sensitivity. Exosomes were isolated from chorionic villous (CV) explants both women Normal Glucose...
Our understanding of how cells communicate has undergone a paradigm shift since the recent recognition role exosomes in intercellular signaling. In this study, we investigated whether oxygen tension alters exosome release and miRNA profile from extravillous trophoblast (EVT) cells, modifying their bioactivity on endothelial (EC). Furthermore, have established exosomal at early gestation women who develop pre-eclampsia (PE) spontaneous preterm birth (SPTB). HTR-8/SVneo were used as an EVT...
Molecules produced by adipose tissue (AT) function as an endocrine link between maternal AT and fetal growth regulating placental in normal women with gestational diabetes mellitus (GDM). We hypothesized that AT-derived exosomes (exo-AT) from GDM would carry a specific set of proteins influences glucose metabolism the placenta. Exosomes were isolated omental AT-conditioned media tolerant (NGT) pregnant (n = 65) 82). Sequential window acquisition all theoretical fragment ion spectra mass...
Vascular tone is controlled by the L-arginine/nitric oxide (NO) pathway, and NO bioavailability strongly affected hyperglycaemia-induced oxidative stress. Insulin leads to high expression activity of human cationic amino acid transporter 1 (hCAT-1), synthesis vasodilation; thus, a protective role insulin on D-glucose–alterations in endothelial function likely. reactivity U46619 (thromboxane A2 mimetic) calcitonin gene related peptide (CGRP) was measured KCl preconstricted umbilical vein...
Several factors including placental hormones (PH) released from the human placenta have been associated with development of insulin resistance and gestational diabetes mellitus (GDM). However, circulating levels PH does not correlate well maternal sensitivity across gestation, suggesting that other, previously unrecognized, mechanisms may be involved. The exosomes are higher in GDM compared to normal. derived produce greater release pro-inflammatory cytokines endothelial cells normal, their...
Exosomes are membrane-bound nanovesicles that transport molecular signals between cells. This study determined changes in maternal plasma exosome proteomics contents term and preterm births. Maternal (MP) samples were collected from group 1: not labor (TNIL, n = 13); 2: (TL, 11); 3: premature rupture of membranes (pPROM, 8); 4: birth (PTB, 13). isolated by differential density centrifugation followed size exclusion chromatography characterized morphology (electron microscopy), quantity...
Abstract Background Gestational diabetes mellitus (GDM) is a serious public health issue affecting 9–15% of all pregnancies worldwide. Recently, it has been suggested that extracellular vesicles (EVs) play role throughout gestation, including mediating placental response to hyperglycaemia. Here, we investigated the EV-associated miRNA profile across gestation in GDM, assessed their utility developing accurate, multivariate classification models, and determined signaling pathways skeletal...
Herein, we developed a specific, rapid sensor to quantify placental extracellular vesicle (EV) protein biomarkers of early pregnancy complications. A distinct tetraspanin CD9 and alkaline phosphatase (PLAP) expression pattern was observed via targeted multiple reaction monitoring EVs from maternal plasma collected before 18 weeks gestation. classification model using training validation patient sets, distinguishing between individuals at high risk developing complications those with normal...
Ovarian cancer has resulted in over 140 000 deaths reported annually worldwide. This is often attributed to cellular changes the microenvironment, including increased migration of mesenchymal stem cells (MSCs) and endothelial (ECs) facilitate metastasis. Recently, ability exosomes communicate signals between (and promote progression) been established. In present study, we explored effect on tumour microenvironment. Exosomes were isolated from ovarian with different invasive capacity (high =...
With five-year survival rates as low 3%, lung cancer is the most common cause of cancer-related mortality worldwide. The severity disease at presentation accredited to lack early detection capacities, resulting in reliance on low-throughput diagnostic measures, such tissue biopsy and imaging. Interest development use liquid biopsies has risen, due non-invasive sample collection, depth information it can provide a disease. Small extracellular vesicles (sEVs) viable are particular interest...
Abstract Exosomes are small nanovesicles that carry bioactive molecules which can be delivered to neighbouring cells modify their biological functions. Studies have showed exosomes from ovarian cancer (OVCA) alter the cell migration and proliferation of within tumour microenvironment, an effect modulated by invasiveness capacity originating cells. Using OVCA line xenograph mouse model, we derived a high (exo-SKOV-3) promoted metastasis in vivo compared with low (exo-OVCAR-3). Analysis anin...
Abstract Extracellular vesicles (EVs), including exosomes, have significant potential for diagnostic and therapeutic applications. The lack of standardized methods efficient high-throughput isolation analysis EVs, however, has limited their widespread use in clinical practice. Surface epitope immunoaffinity (SEI) utilizes affinity ligands, antibodies, aptamers, or lectins, that target specific surface proteins present on EVs. Paramagnetic bead-SEI represents a fit-for-purpose solution the...
Abstract Background The “nonclassic” apparent mineralocorticoid excess (NC-AME) has been identified in approximately 7% of general population. This phenotype is characterized by low plasma renin activity (PRA), high serum cortisol (F) to cortisone (E) ratio, cortisone, Fractional Excretion potassium (FEK) and normal-elevated systolic blood pressure (SBP). An early detection and/or identification novel biomarkers this could avoid the progression or future complications leading arterial...
Accuracy and diagnostic utility of IgM in Bartonella henselae infectionsIntroduction: Laboratory diagnosis cat scratch disease (CSD) is based on the determination specific antibodies anti-Bartonella by different techniques.The CDC recommends IgG immunofluorescent assay (IFA) as gold standard.Objective: To determine accuracy anti-B.henselaeIgM IFA for CSD.Material Methods: Anti-B.henselae was determined serum 108 patients with CSD suspicion; addition, separately blindly two thoroughly trained...
// Qingyan Cui 1 , Frank Vari Alexandre S. Cristino Carlos Salomon 2, 3 Gregory E. Rice 2 Muhammed B. Sabdia Dominic Guanzon Palma Marina Mathew Dipti Talaulikar 4, 5 Sanjiv Jain 4 Erica Han Mark Hertzberg 6 Clare Gould Pauline Crooks Gayathri Thillaiyampalam Colm Keane 1, 7 and Maher K. Gandhi University of Queensland Diamantina Institute, Brisbane, QLD, Australia Centre for Clinical Research, Concepción, Chile Canberra Hospital, Garran, ACT, National Medical School, Prince Wales...
Aim: Saliva extracellular vesicles (EVs) serve as a significant reservoir of biomarkers that may be clinical use in disease diagnosis. Saliva, however, contains EVs both host- and bacterial- origin. Identifying suitable for diagnosis involves enriching host limiting non-host contamination with effective isolation methods. The objectives this research were: (1) to evaluate the salivary enrichment 12 periodontally healthy patients by two different methods: size exclusion chromatography (SEC)...
Abstract Placental extracellular vesicles (EVs) can be found in the maternal circulation throughout gestation, and their concentration, content bioactivity are associated with pregnancy outcomes, including gestational diabetes mellitus (GDM). However, effect of changes microenvironment on mechanisms secretion EVs from placental cells remains to fully established. Here, we evaluated high glucose proteins trafficking release different populations cells. BeWo HTR8/SVneo were used as models...