A5030525901- Cancer Immunotherapy and Biomarkers
- Cancer Genomics and Diagnostics
- Cancer Cells and Metastasis
- Immune cells in cancer
- Single-cell and spatial transcriptomics
- Immunotherapy and Immune Responses
- Ferroptosis and cancer prognosis
- CAR-T cell therapy research
- Radiomics and Machine Learning in Medical Imaging
- Cancer Research and Treatments
- COVID-19 Clinical Research Studies
- Lung Cancer Treatments and Mutations
- Nanoplatforms for cancer theranostics
- interferon and immune responses
- Extracellular vesicles in disease
- Head and Neck Cancer Studies
- Lung Cancer Research Studies
- SARS-CoV-2 and COVID-19 Research
- Metastasis and carcinoma case studies
- 3D Printing in Biomedical Research
- Cancer Diagnosis and Treatment
- Microfluidic and Bio-sensing Technologies
- RNA modifications and cancer
- Tumors and Oncological Cases
- COVID-19 Impact on Reproduction
The University of Queensland
2014-2025
Wesley Hospital
2024-2025
Translational Research Institute
2014-2024
Westmead Institute for Medical Research
2024
University of Münster
2024
Queensland University of Technology
2015-2023
Health First
2021-2023
Princess Alexandra Hospital
2021
Universal Scientific Education and Research Network
2020
Abstract Circulating tumor cells (CTCs) carry a wealth of information on primary and metastatic tumors critical for precise cancer detection, monitoring, treatment. Numerous microfluidic platforms have been developed in the past few years to capture these rare patient bloodstream deciphering needed. However, practical need high-quality method CTC isolation remains be met. Herein, we demonstrate novel multi-flow device that is able sensitively provide high purity (>87%) separation outcome...
Thrombotic and microvascular complications are frequently seen in deceased COVID-19 patients. However, whether this is caused by direct viral infection of the endothelium or inflammation-induced endothelial activation remains highly contentious.Here, we use patient autopsy samples, primary human cells an vitro model pulmonary epithelial-endothelial cell barrier.We show that express very low levels SARS-CoV-2 receptor ACE2 protease TMPRSS2, which blocks their capacity for productive...
Purpose Robust biomarkers that predict disease outcomes amongst COVID-19 patients are necessary for both patient triage and resource prioritisation. Numerous candidate have been proposed COVID-19. However, at present, there is no consensus on the best diagnostic approach to in infected patients. Moreover, it not clear whether such tools would apply other potentially pandemic pathogens therefore of use as stockpile future preparedness. Methods We conducted a multi-cohort observational study...
Abstract Tumor biopsy is the gold standard for assessment of clinical biomarkers treatment. However, tumors change dynamically in response to therapy, and there remains a need more representative biomarker that can be assayed over course Circulating tumor cells (CTCs) may provide clinically important comprehensive tumoral information predictive treatment outcome. Blood samples were processed CTCs from 56 patients using ClearCell FX system. Captured phenotyped CTC clusters markers...
Circulating tumour cells (CTCs) are the metastatic precursors to distant disease in head and neck cancers (HNCs). Whilst prognostic predictive value of single CTCs have been well documented, role CTC clusters, which potentially a higher capacity limited. In this study, authors used novel straight microfluidic chip focus capture CTCs. The offers high cell recoveries with clinically relevant numbers (10⁻500 cells/mL) without need for further purification. Single were identified 10/21 patient...
Head and neck squamous cell carcinomas (HNSCCs) are the seventh most common cancer represent a global health burden. Immune checkpoint inhibitors (ICIs) have shown promise in treating recurrent/metastatic disease with durable benefit ∼30% of patients. Current biomarkers for HNSCC limited their dynamic ability to capture tumor microenvironment (TME) features an increasing need deeper tissue characterization. Therefore, new needed accurately stratify patients predict responses therapy. Here,...
To gain a better understanding of the complexity gene expression in normal and diseased tissues it is important to account for spatial context identity cells situ. State-of-the-art profiling technologies, such as Nanostring GeoMx Digital Spatial Profiler (DSP), now allow quantitative spatially resolved measurement transcriptome tissues. However, bioinformatics pipelines currently used analyse data often fail successfully technical variability within experimental designs, thus limiting...
Mucosal head and neck squamous cell carcinoma (HNSCC) are the seventh most common cancer, with approximately 50% of patients living beyond 5 years. Immune checkpoint inhibitors (ICIs) have shown promising results in recurrent or metastatic (R/M) disease, however, only a subset benefit from immunotherapy. Studies implicated tumor microenvironment (TME) HNSCC as major factor therapy response, highlighting need to better understand TME, particularly by spatially resolved means determine...
Abstract The spatial localisation of immune cells within tumours are key to understand the intercellular communications that can dictate clinical outcomes. Here, we demonstrate an analysis pipeline for highly multiplexed CODEX data phenotype and profile features interactions in NSCLC patients subsequently received PD1 axis immunotherapy. We found regulatory T (Tregs) enriched non-responding this was consistent with their localization stromal peripheral tumour-margins. Proximity-based between...
Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) virus infection in pregnancy is associated with higher incidence of placental dysfunction, referred to by a few studies as 'preeclampsia-like syndrome'. However, the mechanisms underpinning SARS-CoV-2-induced malfunction are still unclear. Here, we investigated whether transcriptional architecture placenta altered response SARS-CoV-2 infection.
Abstract Whilst locoregional control of head and neck cancers (HNCs) has improved over the last four decades, long-term survival remained largely unchanged. A possible reason for this is that rate distant metastasis not changed. Such disseminated disease reflected in measurable levels cancer cells blood HNC patients, referred to as circulating tumour (CTCs). Numerous marker-independent techniques have been developed CTC isolation detection. Recently, microfluidics-based platforms come fore...
Blockade of the PD-1/PD-L1 immune checkpoint pathway is emerging as a promising immunotherapeutic approach for management and treatment head neck cancer patients who do not respond to 1st/2nd line therapy. However, inhibitors are cost intensive, identifying would most likely benefit from anti PD-L1 therapy required. Developing non-invasive technique be major patient health care system. We report case 56 year old man affected by supraglottic squamous cell carcinoma (SCC). A CT scan showed 20...
Abstract Since the beginning of 2020, coronavirus disease (COVID-19) pandemic has dramatically influenced almost every aspect human life. Activities requiring gatherings have either been postponed, canceled, or held completely virtually. To supplement lack in-person contact, people increasingly turned to virtual settings online, advantages which include increased inclusivity and accessibility a reduced carbon footprint. However, emerging online technologies cannot fully replace scientific...
The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) which emerged in late 2019 has spread globally, causing a pandemic of illness designated disease (COVID-19). A better definition the pulmonary host response to SARS-CoV-2 infection is required understand viral pathogenesis and validate putative COVID-19 biomarkers that have been proposed clinical studies.Here, we use targeted transcriptomics formalin-fixed paraffin-embedded tissue using NanoString GeoMX platform generate an...
Triple-negative breast cancer (TNBC) is an aggressive subtype of that has few effective treatment options due to its lack targetable hormone receptors. Whilst the degree tumour infiltrating lymphocytes (TILs) been shown associate with therapy response and prognosis, deeper characterization molecular diversity may mediate chemotherapeutic lacking. Here we applied targeted proteomic analysis both chemotherapy sensitive resistant TNBC tissue samples by Nanostring GeoMx Digital Spatial Platform...