A5054496840- Immune Cell Function and Interaction
- Cytomegalovirus and herpesvirus research
- T-cell and B-cell Immunology
- Cancer Immunotherapy and Biomarkers
- Herpesvirus Infections and Treatments
- Immunotherapy and Immune Responses
- Toxoplasma gondii Research Studies
- Immune cells in cancer
- Ferroptosis and cancer prognosis
- Respiratory viral infections research
- Systemic Lupus Erythematosus Research
- Single-cell and spatial transcriptomics
- Platelet Disorders and Treatments
- Immune Response and Inflammation
- Advanced Biosensing Techniques and Applications
- Asthma and respiratory diseases
- Dermatology and Skin Diseases
- Cancer Research and Treatments
- COVID-19 Clinical Research Studies
- Viral-associated cancers and disorders
- SARS-CoV-2 and COVID-19 Research
- Neonatal Health and Biochemistry
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Immunodeficiency and Autoimmune Disorders
- Allergic Rhinitis and Sensitization
The University of Queensland
2016-2024
Australian National University
2021-2024
Translational Research Institute
2021-2024
Children's Medical Research Institute
2023-2024
In antibody responses, mutated germinal center B (B GC ) cells are positively selected for reentry or differentiation. As the products from GCs, memory and antibody-secreting (ASCs) support high-affinity long-lasting immunity. Positive selection of is controlled by signals received through cell receptor (BCR) follicular helper T (T FH cell–derived signals, in particular costimulation CD40. Here, we demonstrate that effector cytokine interleukin-21 (IL-21) joins BCR CD40 supporting reveal...
Abstract The spatial localisation of immune cells within tumours are key to understand the intercellular communications that can dictate clinical outcomes. Here, we demonstrate an analysis pipeline for highly multiplexed CODEX data phenotype and profile features interactions in NSCLC patients subsequently received PD1 axis immunotherapy. We found regulatory T (Tregs) enriched non-responding this was consistent with their localization stromal peripheral tumour-margins. Proximity-based between...
Abstract Background Recurrent/metastatic head and neck squamous cell carcinoma (R/M HNSCC) generally has a poor prognosis for patients with limited treatment options. While incorporating immune checkpoint inhibitors (ICIs) now become the standard of care, efficacy is variable, only subset responding. The complexity tumor microenvironment (TME) role tertiary lymphoid structures (TLS) have emerged as critical determinants immunotherapeutic response. Methods In this study, we analyzed two...
Abstract Background The contribution of B‐cell subsets and T‐B cell interaction to the pathogenesis allergic rhinitis (AR) mechanisms allergen immunotherapy (AIT) remain poorly understood. This study aimed outline circulating signature, underlying mechanism, its association with clinical response AIT in patients AR. Methods IgD/CD27 CD24/CD38 core gating systems were used determine frequencies phenotypes B cells. Correlations between cells, T antigen‐specific IgE, disease severity AR...
Abstract Objectives We aimed to gain an understanding of the paradox immunity in COVID‐19 patients with T cells showing both functional defects and hyperactivation enhanced proliferation. Methods A total 280 hospitalised were evaluated for cytokine profiles clinical features including viral shedding. mouse model acute infection by lymphocytic choriomeningitis virus (LCMV) was applied dissect relationship between immunological, virological pathological features. The results from validated...
Autoimmune diseases are often treated by glucocorticoids and immunosuppressive drugs that could increase the risk for infection, which in turn deteriorate disease cause mortality. Low-dose IL-2 (Ld-IL2) therapy emerges as a new treatment wide range of autoimmune diseases. To examine its influence on we retrospectively studied 665 patients with systemic lupus erythematosus (SLE) including about one third receiving Ld-IL2 therapy, where was found beneficial reducing incidence infections. In...
Cytomegaloviruses (CMVs) persistently and systemically infect the myeloid cells of immunocompetent hosts. Persistence implies immune evasion, CMVs evade CD8+ T by inhibiting MHC class I-restricted antigen presentation. Myeloid can also interact with CD4+ via II (MHC II). Human CMV (HCMV) attacks presentation pathway in vitro, but what role this evasion might play host colonization is unknown. We show that Murine (MCMV) down-regulates M78, a multi-membrane spanning viral protein captured from...
Cytomegaloviruses (CMVs) establish persistent, systemic infections and cause disease by maternal-foetal transfer, suggesting that their dissemination is a key target for antiviral intervention. Late clinical presentation has meant human CMV (HCMV) not well understood. Murine (MCMV) provides tractable model. Whole mouse imaging of virus-expressed luciferase proved useful way to track infections. MCMV, in which the abundant lytic gene M78 was luciferase-tagged via self-cleaving peptide...
Human cytomegalovirus is a leading cause of congenital disease. This reflects its capacity for systemic spread. A vaccine needed, but the best viral targets are unclear. Attention has focused on virion membrane fusion complex. It 2 forms, so we need to know what each contributes host colonization. One includes glycoprotein O. We used murine cytomegalovirus, which equivalent complexes, determine importance O after mucosal infection. show that it drives local virus replication in epithelial...
Virus homologues of seven-transmembrane receptors (7TMR) are encoded by all beta- and gammaherpesviruses, suggesting important functional roles. M78 mouse cytomegalovirus (MCMV) is representative a family 7TMR conserved in betaherpesviruses. members have been found to exhibit cell-type specific effects upon virus replication tissue culture affect pathogenesis vivo. We reported previously that M78, for which no ligands known, undergoes rapid, constitutive endocytosis. In this study, we...
Summary Cytotoxic CD8 + T cells, essential in combating viral infections and cancer, become dysfunctional from prolonged antigen exposure. Precursors of exhausted (T PEX ) cells are pivotal sustaining immune responses chronic diseases mediating immunotherapy efficacy. They also control infection within B-cell follicles, facilitated by CXCR5 expression. How cytokines regulate cell fate follicular entry is not well understood. We reveal that IL-2 treatment enhances effector functions LCMV but...
Cytotoxic CD8 + T cells, essential in combating viral infections and cancer, become dysfunctional from prolonged antigen exposure. Precursors of exhausted (T PEX ) cells are pivotal sustaining immune responses chronic diseases mediating immunotherapy efficacy. They also control infection within B-cell follicles, facilitated by CXCR5 expression. How cytokines regulate cell fate follicular entry is not well understood. We reveal that IL-2 treatment enhances effector functions LCMV but hinders...
Cytotoxic CD8 + T cells, essential in combating viral infections and cancer, become dysfunctional from prolonged antigen exposure. Precursors of exhausted (T PEX ) cells are pivotal sustaining immune responses chronic diseases mediating immunotherapy efficacy. They also control infection within B-cell follicles, facilitated by CXCR5 expression. How cytokines regulate cell fate follicular entry is not well understood. We reveal that IL-2 treatment enhances effector functions LCMV but hinders...
Abstract Insights into the spatial localisation of immune cells within tumours are key to understand intercellular communications that dictate clinical outcomes. We applied a tiered approach characterise organization and interactions in NSCLC tissues from thirty-five patients subsequently received PD-1 axis immunotherapy (ICI therapy). Our analysis indicates regulatory T enriched non-responding patients. Proximity between Tregs both monocytes(p=0.009) CD8 + cells(p=0.009) were frequently...
Abstract Vaccination against γ‐herpesviruses has proved difficult. CD4 + T cells are essential to contain infection, but how best prime them and whether this can reduce viral loads remain unclear. To address these questions, we used ovalbumin (OVA) as a model antigen, delivering it with murine cytomegalovirus (MCMV) protect mice OVA‐expressing herpesvirus‐4 (MuHV‐4). Membrane‐associated OVA (mOVA) was more effective than soluble OVA, both an effector target. It also better target epitope...
<title>Abstract</title> New-onset HLA-DR-associated anti-citrullinated protein autoantibody (ACPA)<sup>+</sup> rheumatoid arthritis (RA) synovial tissue (ST) contains highly-expanded TCR-αβ clonotypes, some viral-antigen-reactive. However, it is unknown how viral-specific T-cells sustain ACPA<sup>+</sup> RA. We studied paired peripheral blood (PB) and ST TCR repertoires in drug-naïve HLA-DRB1*04:01<sup>+</sup> diffuse-myeloid RA pathology. To model effects of viral infection, we induced...