A5065457962- COVID-19 Clinical Research Studies
- Respiratory Support and Mechanisms
- Inflammasome and immune disorders
- Immune cells in cancer
- Neonatal Respiratory Health Research
- Long-Term Effects of COVID-19
- Heme Oxygenase-1 and Carbon Monoxide
- Osteoarthritis Treatment and Mechanisms
- Mosquito-borne diseases and control
- Biochemical effects in animals
- interferon and immune responses
- Glioma Diagnosis and Treatment
- Neurogenesis and neuroplasticity mechanisms
- Viral Infections and Vectors
- Temporomandibular Joint Disorders
- Neuroblastoma Research and Treatments
- Corneal Surgery and Treatments
- Atherosclerosis and Cardiovascular Diseases
- Neuroinflammation and Neurodegeneration Mechanisms
- Oral Health Pathology and Treatment
- Tendon Structure and Treatment
- Ovarian cancer diagnosis and treatment
- Retinal Diseases and Treatments
- Vector-Borne Animal Diseases
- Salivary Gland Tumors Diagnosis and Treatment
Pontifícia Universidade Católica do Paraná
2014-2025
Universidade Federal do Paraná
2017-2022
Hospital de Clínicas Universidade Federal do Paraná
2020
Objective: Alveolar-capillary endothelial cells can be activated by severe acute respiratory syndrome coronavirus 2 infection leading to cytokine release. This could trigger dysfunction, pyroptosis, and thrombosis, which are the vascular changes, commonly referred as disease 2019 (COVID-19) endotheliopathy. Thus, this study aimed identify tissue biomarkers associated with activation/dysfunction pyroptosis pathway in lung samples of patients COVID-19 compare them pandemic influenza A virus...
Abstract The COVID-19 fatality rate is high when compared to the H1N1pdm09 (pandemic Influenza A virus H1N1 subtype) rate, and although both cause an aggravated inflammatory response, differences in mechanisms of pandemic pneumonias need clarification. Thus, our goal was analyze tissue expression interleukins 4, 13, (IL-4, IL-13), transforming growth factor-beta (TGF-β), number M2 macrophages (Sphingosine-1) patients who died by COVID-19, comparing with cases severe pneumopathy caused...
It is currently believed that innate immunity unable to prevent the spread of SARS-CoV-2 from upper airways alveoli high-risk groups patients. replication in ACE-2-expressing pneumocytes can drive diffuse alveolar injury through cytokine storm and immunothrombosis by upregulating transcription chemokine/cytokines, unlike several other respiratory viruses. Here we report histopathology data obtained post-mortem lung biopsies COVID-19, showing increased density perivascular septal mast cells...
Acute respiratory distress syndrome (ARDS) followed by repair with lung remodeling is observed in COVID-19. These findings can lead to pulmonary terminal fibrosis, a form of irreversible sequelae. There evidence that TGF-β intimately involved the fibrogenic process. When activated, promotes differentiation fibroblasts into myofibroblasts and regulates extracellular matrix (ECM). In this sense, present study evaluated histopathological features immunohistochemical biomarkers (ACE-2, AKT-1,...
MicroRNAs (miRNAs) are critical modulators of endothelial homeostasis, which highlights their involvement in vascular diseases, including those caused by virus infections. Our main objective was to identify miRNAs involved the function and determine expression post-mortem lung biopsies COVID-19 patients with severe respiratory injuries thrombotic events. Based on functional enrichment analysis, miR-26a-5p, miR-29b-3p, miR-34a-5p were identified as regulators mRNA targets inflammatory...
The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) which emerged in late 2019 has spread globally, causing a pandemic of illness designated disease (COVID-19). A better definition the pulmonary host response to SARS-CoV-2 infection is required understand viral pathogenesis and validate putative COVID-19 biomarkers that have been proposed clinical studies.Here, we use targeted transcriptomics formalin-fixed paraffin-embedded tissue using NanoString GeoMX platform generate an...
Abstract Orthobunyavirus oropouche ense virus (OROV), the causative agent of Oropouche fever, is widely dispersed in Brazil and South America, causing sporadic outbreaks. Due to similarity initial clinical symptoms caused by OROV with other arboviruses found overlapping geographical areas, differential diagnosis challenging. As for most neglected tropical diseases, there a shortage reagents diagnosing studying pathogenesis. We therefore developed characterized mouse monoclonal antibodies...
Mast cells (MCs) have relevant participation in inflammatory and vascular hyperpermeability events, responsible for the action of kallikrein-kinin system (KKS), that affect patients inflicted by severe form COVID-19. Given a higher number activated MCs present COVID-19 their association with we investigated factors lead to activation degranulation these harmful effects on alveolar septum environment provided its mediators. Therefore, pyroptotic processes throughout caspase-1 (CASP-1) alarmin...
Chikungunya virus (CHIKV), the causative agent of chikungunya fever, is an alphavirus widely transmitted by bite female mosquito genus Aedes sp., especially in tropical and subtropical regions. Brazil country most affected microorganism. CHIKV classically induces articular pain, which can become long lasting for even years a great number infected individuals, reducing their quality life. The mechanisms CHIKV-induced pain are poorly understood, but recent evidence indicated role transient...
Obesity has been identified as an independent risk factor for severe COVID-19 unfavorable outcomes. Several factors, such increased ACE2 receptor expression and chronic inflammation, can contribute to this relationship, yet the activation of NLRP3 inflammasome pathway is also a key element. Our primary goal was determine whether in people with obesity different critical conditions. A retrospective analysis conducted using clinical data post-mortem lung tissue samples from 14 patients (group...
Achilles tendon injuries are extremely common and have a significant impact on the physical mental health of individuals. Both conservative surgical treatments unsatisfactory results. The search for new therapeutic tools, using cell therapies with stem cells (SC) biological tissues, such as amniotic membranes (AM), has proved useful regeneration injured tendons. Background/Objectives: This research was carried out to assess capacity tissue repair in animal models tendinopathy, which rats...
Objective The objective was to evaluate the early-stage immunoexpression of markers (COX-2, NF-kB, VEGFR-1 and apoptotic index) related inflammation, angiogenesis, cell survival in tongue dorsum epithelium mice exposed hookah smoke. Materials methods sample consisted Swiss (N = 20), female gender, aged 2 months, approximately 25g each, four groups (n 5) mice: group fresh air smoke for 7, 15, 30 days. Tongues were embedded paraffin. A tissue microarray constructed, immunohistochemistry...
Abstract We developed Standardised Minimum 3D Distance (SM3DD), an entirely cell segmentation/annotation-free approach to the analysis of spatial RNA datasets, using it compare lung tissue from 16 clinically normal individuals those 18 SARS-CoV-2 patients who died acute respiratory distress syndrome. coordinates were determined CosMx™ Spatial Molecular Imager (Bruker Biology, US). For each individual transcript location, we calculated three-dimensional distances nearest type, standardising...
Several studies have focused on novel therapeutic strategies for extensive skin lesions aiming to improve healing quality and reduce treatment duration. In this context, the use of amniotic membrane (AM) Wharton's jelly (WJ) emerges as promising alternatives. Full-thickness dorsal wounds were created in 21 Wistar rats, randomly divided into three groups: control (C), AM - covered by WJ WJ. Wound contraction rate was measured weekly. On day 28, histochemical (Picrosirius red)...
The COVID-19 pandemic is a worldwide threat, and information on physiopathological aspects of the disease limited. Despite efforts in searching treatment options, better understanding SARS-CoV-2 pathways can contribute to managing severe cases. In this study, we aim describe pathological immunopathogenic findings two different cases, both high-risk group. Post-mortem lung biopsies were analyzed by traditional immunohistochemical methods. Tissue expression innate adaptive immune response...
Nephroblastoma or Wilms tumor is the most frequent kidney cancer in children and accounts for 98% of tumors this age group. Despite favorable prognosis, a subgroup these patients progresses to recurrence death. The retinoic acid (RA) pathway plays role chemoprevention treatment due its effects on cell differentiation antiproliferative, anti-oxidant, pro-apoptotic activities. Reports describe abnormal cellular acid-binding protein 2 (CRABP2) expression neoplasms correlation with prognostic...
The inflammasome complex is a key part of chronic diseases and acute infections, being responsible for cytokine release cell death mechanism regulation. SARS-CoV-2 infection characterized by dysregulated release. In this context, the analysis within may prove beneficial to understand disease’s mechanisms. Post-mortem minimally invasive autopsies were performed in patients who died from COVID-19 (n = 24), lung samples compared patient control group 11) an Influenza A virus H1N1 subtype 2009...
Abstract Background: The COVID-19 fatality rate is high when compared to the H1N1pdm09 (pandemic Influenza A virus H1N1 subtype) rate, and although both cause an aggravated inflammatory response, differences in mechanisms of pandemic pneumonias need clarification. Objective: To analyze tissue expression interleukins 4, 13, (IL-4, IL-13), transforming growth factor-beta (TGF-β), number M2 macrophages (Sphingosine-1) patients who died by COVID-19, comparing with cases severe pneumopathy caused...
This study aims to evaluate and compare cellular therapy with human Wharton’s jelly (WJ) mesenchymal stem cells (MSCs) neural precursors (NPs) in experimental autoimmune encephalomyelitis (EAE), a preclinical model of Multiple Sclerosis. MSCs were isolated from WJ by an explant technique, differentiated NPs, characterized cytometry immunocytochemistry analysis after ethical approval. Forty-eight rats EAE-induced myelin basic protein Freund’s complete adjuvant. hours later, the animals...
Abstract Objective This study was conducted to correlate and compare the immunoexpression of sex‐determining region Y‐box 2 ( SOX ‐2) in oral leukoplakia OL ) lesions with that normal buccal mucosa (control). Materials Methods In this observational study, low‐risk n = 34) high‐risk 33) dysplasia control samples 25) were subjected immunohistochemical analysis for ‐2. epithelium, ‐2 positive negative cells, as well semiautomatic segmentation immunopositive nuclear area counted. Statistical...