A5012947375- Ion Channels and Receptors
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Pain Mechanisms and Treatments
- Essential Oils and Antimicrobial Activity
- Mast cells and histamine
- Neurobiology and Insect Physiology Research
- Urticaria and Related Conditions
- Pomegranate: compositions and health benefits
- Cardiovascular, Neuropeptides, and Oxidative Stress Research
- Pharmacological Effects of Natural Compounds
- Phytochemical compounds biological activities
- Olfactory and Sensory Function Studies
- Neuropeptides and Animal Physiology
- Biochemical Analysis and Sensing Techniques
- Genomics, phytochemicals, and oxidative stress
- Dental Erosion and Treatment
- Dermatology and Skin Diseases
- Gut microbiota and health
- Natural product bioactivities and synthesis
- Phytochemicals and Antioxidant Activities
- Probiotics and Fermented Foods
- Traditional and Medicinal Uses of Annonaceae
- Blood Coagulation and Thrombosis Mechanisms
- Piperaceae Chemical and Biological Studies
- Silymarin and Mushroom Poisoning
Instituto de Pesca
2021-2025
Instituto de Tecnologia do Paraná
2020-2023
Homi Bhabha National Institute
2023
Tata Memorial Hospital
2020-2023
Centro Universitário das Faculdades Metropolitanas Unidas
2022
Centro Universitário do Maranhão
2014-2021
Universidade Federal do Maranhão
2020
Brazilian National Association of Graduate Programs in Communication
2018
Centro Científico Tecnológico - San Luis
2018
Brazilian Academy of Sciences
2018
To investigate the involvement of transient receptor potential ankyrin 1 (TRPA1) in inflammatory hyperalgesia mediated by tumor necrosis factor α(TNFα) and joint inflammation.Mechanical was assessed CD1 mice, mice lacking functional TRP vanilloid (TRPV1-/-) or TRPA1 (TRPA1-/-), respective wildtype (WT) mice. An automated von Frey system used, following unilateral intraplantar injection TNFα intraarticular Freund's complete adjuvant (CFA). Knee swelling histologic changes were determined...
The transient receptor potential vanilloid 1 (TRPV1) is primarily localized to sensory nerve fibers and associated with the stimulation of pain inflammation. TRPV1 knockout (TRPV1KO) mice show enhanced LPS-induced sepsis compared wild type (WT). This implies that may have a key modulatory role in increasing beneficial reducing harmful components sepsis. We investigated immune inflammatory mechanisms cecal ligation puncture (CLP) model over 24 h. CLP TRPV1KO exhibited significant hypothermia,...
The cold-induced vascular response, consisting of vasoconstriction followed by vasodilatation, is critical for protecting the cutaneous tissues against cold injury. Whilst this physiological reflex response historic knowledge, mechanisms involved are unclear. Here using a murine model local environmental exposure, we show that TRPA1 acts as primary sensor, determined through pharmacological antagonism or gene deletion. initial mediated via TRPA1-dependent superoxide production stimulates...
α-Calcitonin gene-related peptide (αCGRP) is a vasodilator, but there limited knowledge of its long-term cardiovascular protective influence. We hypothesized that αCGRP protects against the onset and development angiotensin II-induced hypertension have identified mechanisms at vascular level. Wild-type knockout mice similar baseline blood pressure were investigated in II model for 14 28 days. exhibited enhanced aortic hypertrophy. gene expression was increased dorsal root ganglia conduit...
The present study evaluated the participation of tumour necrosis factor‐ α (TNF‐ ) in inflammatory and nociceptive responses evoked by carrageenan mouse paw. intraplantar injection (300 μ g paw −1 induced a marked biphasic oedema formation (peaks at 6 72 h), which was accompanied long‐lasting mechanical allodynia (that remained elevated for up to h) significant increase myeloperoxidase (MPO) activity (peak both Swiss C57/BL6 mice. oedema, elevation MPO lesser extent elicited were found be...
Inflammatory diseases associated with pain are often difficult to treat in the clinic due insufficient understanding of nociceptive pathways involved. Recently, there has been considerable interest role reactive oxygen species (ROS) inflammatory disease, but little is known hydrogen peroxide (H2O2) hyperalgesia. In present study, intraplantar injection H2O2-induced a significant dose- and time-dependent mechanical thermal hyperalgesia mouse hind paw, increased c-fos activity observed dorsal...
Obesity induced by Western diets is associated with type 2 diabetes mellitus and cardiovascular diseases, although underlying mechanisms are unclear. We investigated a murine model of diet-induced obesity to determine the effect transient potential receptor vanilloid 1 (TRPV1) deletion on hypertension metabolic syndrome. Wild-type TRPV1 knockout mice were fed normal or high-fat diet from 3 15 weeks. High-fat diet-fed both genotypes became obese, similar increases in body adipose tissue...
Vascular endothelial growth factor-A (VEGF-A) is best known as a key regulator of the formation new blood vessels. Neutralization VEGF-A with anti-VEGF therapy e.g. bevacizumab, can be painful, and this hypothesized to result from loss VEGF-A-mediated neuroprotection. The multiple vegf-a gene products consist two alternatively spliced families, typified by VEGF-A165a VEGF-A165b (both contain 165 amino acids), both which are neuroprotective. Under pathological conditions, such in inflammation...
Abstract Background and Purpose Transient receptor potential ankyrin‐1 (TRPA1) activation is known to mediate neurogenic vasodilatation. We investigated the mechanisms involved in TRPA1‐mediated peripheral vasodilatation vivo using TRPA1 agonist cinnamaldehyde. Experimental Approach Changes vascular ear blood flow were measured anaesthetized mice laser Doppler flowmetry. Key Results Topical application of cinnamaldehyde mouse caused a significant increase skin wild‐type (WT) but not knockout...
Many Clostridium species are found as commensal members of the intestinal microbiota. However, imbalances microbiota may lead to certain infections caused by these microorganisms, mainly butyricum, difficile, and perfringens. In many cases, infection recurrence can occur after antibiotics, indicating need for novel therapeutic options that act on pathogens also restore Herein, in vitro antimicrobial activity probiotic potential clinical reference strains Bifidobacterium Lactobacillus were...
Cinnamaldehyde is a natural essential oil suggested to possess anti-bacterial and anti-inflammatory properties; activate transient receptor potential ankyrin 1 (TRPA1) channels expressed on neuronal non-neuronal cells. Here, we investigated the immunomodulatory effects of cinnamaldehyde in an vivo model systemic inflammatory response syndrome (SIRS) induced by lipopolysaccharide. Swiss mice received single oral treatment with h before LPS injection. To investigate whether are dependent TRPA1...
Bacterial resistance to the available marketed drugs has prompted search of novel therapies; especially in regards anti-virulence strategies that aim make bacteria less pathogenic and/or decrease their probability become resistant therapy. Cinnamaldehyde is widely known for its antibacterial properties through mechanisms include interaction this compound with bacterial cell walls. However, only a handful studies have addressed effects on virulence, when tested at sub-inhibitory...
This randomized double-blind clinical trial compared tooth sensitivity (TS), bleaching efficacy, and cytokine levels after applying in-office treatments containing 15% 35% hydrogen peroxide (HP15% HP35%, respectively). Twenty-five volunteers were randomly assigned to receive HP15% or HP35% treatment. The agent was applied in three 15-min applications per session. Two sessions separated by a 1-week interval. participants scored TS using visual analog scale numerical rating scale. Bleaching...
Terminalia catappa Linn bark is used to treat dysentery by various populations in Southeast Asian countries, and its leaves have also been traditional medicine hepatitis India the Philippines. Here, antifungal actions of crude hydro-alcoholic extract (TcHE) fractions from T. were assessed via agar diffusion microdilution tests on Candida reference strains clinical isolates patients with acquired immunodeficiency syndrome (AIDS). Additionally, potential cytotoxic effects TcHE cultured human...
Escherichia coli and Staphylococcus aureus are important agents of urinary tract infections that can often evolve to severe infections. The rise antibiotic-resistant strains has driven the search for novel therapies replace use or act as adjuvants antibiotics. In this context, plant-derived compounds have been widely investigated. Cuminaldehyde is suggested major antimicrobial compound cumin seed essential oil. However, effect not fully understood. Herein, we investigated in silico vitro...
Abstract Several studies have now clearly established the ability of LPS to induce bradykinin B1 receptor up-regulation in vivo and functional relevance this for pathophysiological effects LPS. Using an system which is injected locally into rat paw, we examined potential contribution proinflammatory cytokines, NF-κB activation, neutrophil influx molecular receptor. Treatment with resulted a rapid sustained receptors paw that correlated increase mRNA levels. preceded by activation...
Transient potential vanilloid 1 (TRPV1) receptor is an ion channel primarily localized on sensory nerves and activated by specific stimuli to initiate amplify pain inflammation, as typified murine models of scald arthritis. Little known the role TRPV1 in sepsis, infective disease associated with inflammation. Through use a sublethal model lipopolysaccharide-induced peritoneal we provide novel evidence that genetic deletion leads enhanced onset various pathological components systemic...
We investigated the mechanisms underlying pruritogenic response induced by trypsin in mice, to assess relevance of neurogenic inflammation components this response.Itching was an intradermal injection mouse neck. The animals were observed for 40 min and their scratching behaviour quantified.Trypsin-induced itching blocked lima bean inhibitor, selective proteinase-activated receptor-2 (PAR-2) antagonist FSLLRY PAR-2 receptor desensitization. An important involvement mast cells observed, as...