A5084875749- Escherichia coli research studies
- Antibiotic Resistance in Bacteria
- Gut microbiota and health
- Enterobacteriaceae and Cronobacter Research
- Agricultural Economics and Policy
- Urinary Tract Infections Management
- Bacteriophages and microbial interactions
- Viral gastroenteritis research and epidemiology
- Vibrio bacteria research studies
- Bacterial Genetics and Biotechnology
- Probiotics and Fermented Foods
- Clostridium difficile and Clostridium perfringens research
- Bacterial biofilms and quorum sensing
- Amoebic Infections and Treatments
- Listeria monocytogenes in Food Safety
- Genomics and Phylogenetic Studies
- Salmonella and Campylobacter epidemiology
- Infections and bacterial resistance
- Immune Response and Inflammation
- Aquaculture disease management and microbiota
- Microbial infections and disease research
- Vector-borne infectious diseases
- Thyroid Cancer Diagnosis and Treatment
- Parasitic Infections and Diagnostics
- Pancreatitis Pathology and Treatment
Statens Serum Institut
2013-2023
Chr. Hansen (Denmark)
2021-2023
John Deere (Germany)
2023
ALK-Abelló (Denmark)
2022
Bombardier (Canada)
2009
Kiel University
1984-2008
State University of New York
1998
Tel Aviv University
1998
New York University
1998
University of Tennessee at Knoxville
1998
A large outbreak of diarrhea and the hemolytic-uremic syndrome caused by an unusual serotype Shiga-toxin-producing Escherichia coli (O104:H4) began in Germany May 2011. As July 22, a number cases E. have been reported--3167 without (16 deaths) 908 with (34 deaths)--indicating that this strain is notably more virulent than most strains. Preliminary genetic characterization suggested that, unlike these strains, it should be classified within enteroaggregative pathotype coli.We used...
ABSTRACT Highly invasive, community-acquired Klebsiella pneumoniae infections have recently emerged, resulting in pyogenic liver abscesses. These are caused by hypervirulent K. (hvKP) isolates primarily of capsule serotype K1 or K2. Hypervirulent belong to clonal complex 23 (CC23), indicating that this lineage has a specific genetic background conferring hypervirulence. Here, we apply whole-genome sequencing collection characterize the phylogenetic hvKP with an emphasis on CC23. Most...
ABSTRACT Pathogens of the genus Klebsiella have been classified into distinct capsular (K) types for nearly a century. K typing species still has important applications in epidemiology and clinical microbiology, but serological method strong practical limitations. Our objective was to evaluate sequencing wzi , gene conserved all pneumoniae that codes an outer membrane protein involved capsule attachment cell surface, as simple rapid prediction type. The 447-nucleotide region distinguished...
The degree to which molecular epidemiology reveals information about the sources and transmission patterns of an outbreak depends on resolution technology used samples studied. Isolates Escherichia coli O104:H4 from centered in Germany May-July 2011, much smaller southwest France June were indistinguishable by standard tests. We report a epidemiological analysis using multiplatform whole-genome sequencing multiple isolates German French outbreaks. showed remarkably little diversity, with...
Conventional wisdom regarding mechanisms of bacterial pathogenesis holds that pathogens arise by external acquisition distinct virulence factors, whereas determinants shared and commensals are considered to be functionally equivalent have been ignored as genes could become adapted specifically for virulence. It is shown here, however, genetic variation in an originally commensal trait, the FimH lectin type 1 fimbriae, can change tropism Escherichia coli , shifting it toward a urovirulent...
We report here the sequencing and analysis of genome nitrogen-fixing endophyte, Klebsiella pneumoniae 342. Although K. 342 is a member enteric bacteria, it serves as model for studies endophytic, plant-bacterial associations due to its efficient colonization plant tissues (including maize wheat, two most important crops in world), while maintaining mutualistic relationship that encompasses supplying organic nitrogen host plant. Genomic examined presence previously identified genes from other...
Abstract Background Klebsiella pneumoniae is an important gram-negative opportunistic pathogen causing primarily urinary tract infections, respiratory and bacteraemia. The ability of bacteria to form biofilms on medical devices, e.g. catheters, has a major role in development many nosocomial infections. Most clinical K. isolates express two types fimbrial adhesins, type 1 fimbriae 3 fimbriae. In this study, we characterized the biofilm formation. Results Isogenic mutants isolate C3091 were...
Klebsiella pneumoniae is recognized as an important gram-negative opportunistic pathogen. The ability of bacteria to adhere host structures considered essential for the development infections; however, few studies have examined influence adhesion factors on K. virulence. In this study, we cloned and characterized type 1 fimbria gene cluster a clinical isolate. Although was not identical Escherichia coli cluster, overall high degree structural resemblance demonstrated. Unique fim fimK gene,...
ABSTRACT Enteroaggregative Escherichia coli (EAEC) is an important cause of diarrhea worldwide. We analyzed 17 Danish EAEC strains, isolated in the course a case control study, for phenotypic and genotypic properties. The strains belonged to at least 14 different serotypes. Using PCR investigate prevalence various putative virulence genes, we found that all but two were typical EAEC, as they harbored or part previously described AggR regulon. majority genes encoding aggregative adherence...
Urinary catheters are standard medical devices utilized in both hospital and nursing home settings, but associated with a high frequency of catheter-associated urinary tract infections (CAUTI). In particular, biofilm formation on the catheter surface by uropathogens such as Klebsiella pneumoniae causes severe problems. Here we demonstrate that type 1 3 fimbriae expressed K. enhance catheterized bladder model mirrors physico-chemical conditions present patients. Furthermore, show fimbrial...
The rise of antibiotic-resistant Klebsiella pneumoniae, a leading nosocomial pathogen, prompts the need for alternative therapies. We have identified and characterized novel depolymerase enzyme encoded by phage KP36 (depoKP36), from Siphoviridae family. To gain insights into catalytic structural features depoKP36, we recombinantly produced this protein 93.4 kDa showed that it is able to hydrolyze crude exopolysaccharide K. pneumoniae host. Using in vitro vivo assays, found depoKP36 was also...
Summary Klebsiella pneumoniae is an important opportunistic pathogen and a frequent cause of nosocomial infections. K. infections can occur at nearly any body site; however, urinary tract the respiratory predominate. Infections are frequently preceded by gastrointestinal colonization, believed to be most reservoir for transmission bacteria. In contrast many other bacterial pathogens, ubiquitous in nature. Several studies have described isolates environmental origin identical clinical with...
ABSTRACT A model of ascending unobstructed urinary tract infection (UTI) in mice was developed to study the significance antibiotic concentration urine, serum, and kidney tissue for efficacy treatment UTI general pyelonephritis particular. Outbred Ssc-CF1 female were used throughout study, Escherichia coli as pathogen. The virulence 11 uropathogenic E. isolates 1 nonpathogenic laboratory strain examined. Strain C175-94 achieved highest counts kidneys, this subsequently infecting organism....
Mutant screens and transcriptome studies led us to consider whether the metabolism of glucose polymers, i.e., maltose, maltodextrin, glycogen, is important for Escherichia coli colonization intestine. By using streptomycin-treated mouse model, we found that catabolism disaccharide maltose provides a competitive advantage in vivo pathogenic E. O157:H7 commensal K-12, whereas degradation exogenous forms more complex polymer, does not. The endogenous appears play an role colonization, since...
Type 3 fimbriae are expressed by most clinical Klebsiella pneumoniae isolates and mediate adhesion to host structures in vitro. However, the role of type K. virulence has not been evaluated use vivo infection models. In this study, fimbrial gene cluster (mrk) isolate C3091 is described detail. The mrk was revealed be localized close proximity 1 cluster. Thus, a 20.4-kb fimbria-encoding region identified found highly conserved among different isolates. Interestingly, homologue PecS, known as...
ABSTRACT We report the characteristics of 115 extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli clinical isolates, from unique Danish patients, over a 1-year study interval (1 October 2008 to 30 September 2009). Forty-four (38%) ESBL isolates represented sequence type 131 (ST13)1, phylogenetic group B2. The remaining 71 were groups D (27%), A (22%), B1 (10%), and B2 (3%). Serogroup O25 ST131 ( n = 42; 95% ST131) comprised 7 different K antigens, whereas two O16:K100:H5....
ABSTRACT Enteroaggregative Escherichia coli (EAEC) organisms belong to a diarrheagenic pathotype known cause diarrhea and can be characterized by distinct aggregative adherence (AA) in stacked-brick pattern cultured epithelial cells. In this study, we investigated 118 EAEC strains isolated from the stools of Danish adults with traveler's diarrhea. We evaluated presence fimbriae (AAFs) multiplex PCR, targeting four major subunit variants as well their usher-encoding genes. Almost one-half...
A multiresistant clonal Escherichia coli O78:H10 strain qualifying molecularly as enteroaggregative (EAEC) was recently shown to be the cause of a community-acquired outbreak urinary tract infection (UTI) in greater Copenhagen, Denmark, 1991. This marks first time EAEC has been associated with an extraintestinal disease outbreak. Importantly, isolates were recovered from urine patients symptomatic UTI, strongly implying urovirulence. Here, we sought determine uropathogenic properties...
Bacterial resistance to the available marketed drugs has prompted search of novel therapies; especially in regards anti-virulence strategies that aim make bacteria less pathogenic and/or decrease their probability become resistant therapy. Cinnamaldehyde is widely known for its antibacterial properties through mechanisms include interaction this compound with bacterial cell walls. However, only a handful studies have addressed effects on virulence, when tested at sub-inhibitory...
There are challenges, when extracting bacterial DNA from specimens for molecular diagnostics, since fecal samples also contain human cells and many different substances derived food, cell residues medication that can inhibit downstream PCR. The purpose of the study was to evaluate two extraction methods in order choose most efficient method studying intestinal diversity using Denaturing Gradient Gel Electrophoresis (DGGE). In this study, a semi-automatic system (easyMag®, BioMérieux, Marcy...
Objectives and methodsKlebsiella pneumoniae is a nosocomial pathogen considered the most common Gram-negative bacterium that exhibits multiple antimicrobial resistances. In this study, transfer of resistance genes from clinical multiresistant K. MGH75875 isolate was assessed in vitro vivo an intestinal colonization animal model. The ability to colonize tested under different treatments. frequency horizontal gene also examined vitro.
Klebsiella pneumoniae is an important opportunistic pathogen and a frequent cause of nosocomial infections. We have characterized K. strain responsible for series critical infections in intensive care unit over two-year period. The was found to be remarkably thermotolerant providing conceivable explanation its persistence the hospital environment. This marked phenotype mediated by novel type Clp ATPase, designated ClpK. clpK gene encoded conjugative plasmid we find that alone renders...
The potential of Escherichia coli (E. coli) isolated from inflammatory bowel disease (IBD) patients to damage the integrity intestinal epithelium was investigated.E. strains with ulcerative colitis (UC) and healthy controls were tested for virulence capacity by molecular techniques cytotoxic assays transepithelial electric resistance (TER). E. isolate p19A selected, deletion mutants created alpha-hemolysin (α-hemolysin) (hly) clusters necrotizing factor type 1 (cnf1). Probiotic Nissle...