A5024235580- Caveolin-1 and cellular processes
- Cell Adhesion Molecules Research
- Signaling Pathways in Disease
- Lung Cancer Treatments and Mutations
- Peptidase Inhibition and Analysis
- Cancer Cells and Metastasis
- Protein Tyrosine Phosphatases
- 3D Printing in Biomedical Research
- Metabolism, Diabetes, and Cancer
- Cancer-related gene regulation
- ATP Synthase and ATPases Research
- Microtubule and mitosis dynamics
- Cellular Mechanics and Interactions
- Mitochondrial Function and Pathology
- Cellular transport and secretion
- Lung Cancer Research Studies
- Bone health and treatments
- Cancer, Hypoxia, and Metabolism
- Hepatocellular Carcinoma Treatment and Prognosis
- Nerve injury and regeneration
- Amyotrophic Lateral Sclerosis Research
- TGF-β signaling in diseases
- Research on Leishmaniasis Studies
- Autophagy in Disease and Therapy
- Obesity, Physical Activity, Diet
University of Chile
2014-2024
Advanced Center for Chronic Diseases
2014-2024
Universitat de Barcelona
2019-2024
Institute for Bioengineering of Catalonia
2023
Hospital Jerez Puerta del Sur
2019
Institut Català d'Oncologia
2019
Hospital Universitario Virgen del Rocío
2019
Research Institute Hospital 12 de Octubre
2019
Hospital Clínico Universitario Virgen de la Victoria
2019
Hospital Universitario Virgen Macarena
2019
Early in cancer development, tumour cells express vascular endothelial growth factor (VEGF), a secreted molecule that is important all stages of angiogenesis, an essential process provides nutrients and oxygen to the nascent tumor thereby enhances tumor-cell survival facilitates growth. Survivin, another protein involved strongly expressed most human cancers, where it promotes by reducing apoptosis as well favoring cell proliferation migration. The mechanisms which induce VEGF expression...
Motor neurons are progressively and predominantly degenerated in ALS, which is not only induced by multiple intrinsic pathways but also significantly influenced the neighboring glial cells. In particular, astrocytes derived from SOD1 mutant mouse model of ALS or human familial sporadic patient brain tissue directly induce motor neuron death culture; however, mechanisms pathological astroglial secretion remain unclear. Here we investigated abnormal calcium homeostasis altered exocytosis...
Rab5 is a small GTPase that regulates early endosome trafficking and other cellular processes, including cell adhesion migration. Specifically, promotes Rac1 activation cancer migration, but little known about upstream regulators of Rab5. We have previously shown the scaffolding protein Caveolin-1 (CAV1) migration cells. Here, we hypothesized CAV1 stimulates activation, leading to increased activity Expression in B16-F10 mouse melanoma HT-29(US) human colon adenocarcinoma cells Rab5-GTP...
Breast cancer is one of the most frequently diagnosed cancers and leading cause cancer-related deaths in women worldwide, whereby mortality largely attributable to development distant metastasis. Caveolin-1 (CAV1) a multifunctional membrane protein that typically upregulated final stages promotes migration invasion tumor cells. Elevated levels CAV1 have been detected extracellular vesicles (EVs) from advanced patients. EVs are lipid enclosed vesicular structures contain bioactive proteins,...
Close contacts between endoplasmic reticulum and mitochondria enable reciprocal Ca2+ exchange, a key mechanism in the regulation of mitochondrial bioenergetics. During early phase stress, this inter-organellar communication increases as an adaptive to ensure cell survival. The signalling pathways governing response, however, have not been characterized. Here we show that caveolin-1 localizes reticulum–mitochondria interface, where it impairs remodelling contacts, quenching transfer rendering...
Caveolin-1 is known to promote cell migration, and increased caveolin-1 expression associated with tumor progression metastasis. In fibroblasts, polarization phosphorylation of tyrosine-14 are essential migration. However, the role in migration metastatic cells remains poorly defined. Here, participation was evaluated by shRNA targeting endogenous MDA-MB-231 human breast cancer ectopic B16-F10 mouse melanoma cells. Depletion reduced, while promoted focal adhesion turnover a sequence events...
Summary The role of caveolin‐1 ( CAV 1) in cancer is highly controversial. 1 suppresses genes that favor tumor development, yet also promotes focal adhesion turnover and migration metastatic cells. How these contrasting observations relate to function vivo unclear. Our previous studies implicate E ‐cadherin 1‐dependent suppression. Here, we use murine melanoma B 16 F 10 cells, with low levels endogenous ‐cadherin, unravel how affects growth metastasis assess co‐expression modulates C 57 BL...
Caveolin-1 (CAV1) is a scaffolding protein that plays dual role in cancer. In advanced stages of this disease, CAV1 expression tumor cells associated with enhanced metastatic potential, while, at earlier stages, functions as suppressor. We recently implicated phosphorylation on tyrosine 14 (Y14) CAV1-enhanced cell migration. However, the contribution modification to cancer remained unexplored. Here, we used vitro [2D and transendothelial migration (TEM), invasion] vivo (metastasis) assays,...
Tissue inhibitor of metalloproteinase-1 (TIMP-1) is an important regulator extracellular matrix turnover that has been traditionally regarded as a potential tumor suppressor owing to its inhibitory effects metalloproteinases. Intriguingly, this interpretation challenged by the consistent observation increased expression TIMP-1 associated with poor prognosis in virtually all cancer types including lung cancer, supporting tumor-promoting function. However, how dysregulated within...
Helicobacter pylori is the etiologic agent of a series gastric pathologies that may culminate in development adenocarcinoma. An initial step this process loss glandular structures mucosa, presumably as consequence increased apoptosis and reduced cellular regeneration, which be attributed to combination several bacterial host factors an unfavorable proinflammatory environment. In previous study, we showed survivin, member inhibitor protein family, expressed normal human mucosa its levels...
Caveolin-1 (CAV1) has been implicated both in tumor suppression and progression, whereby the specific role appears to be context dependent. Endometrial cancer is one of most common malignancies female genital tract; however, little known about CAV1 this disease. Here, we first determined by immunohistochemistry protein levels normal proliferative human endometrium endometrial samples. Then using two cell lines (ECC: Ishikawa Hec-1A) evaluated mRNA real time qPCR Western blot analysis,...
Cancer cells often display impaired mitochondrial function, reduced oxidative phosphorylation, and augmented aerobic glycolysis (Warburg effect) to fulfill their bioenergetic biosynthetic needs. Caveolin-1 (CAV1) is a scaffolding protein that promotes cancer cell migration, invasion, metastasis in manner dependent on CAV1 phosphorylation tyrosine-14 (pY14). Here, we show expression increased rates, while respiration was by inhibition of the complex IV. These effects correlated with reactive...
Mechanical changes in tumors have long been linked to increased malignancy and therapy resistance attributed mechanical the tumor extracellular matrix (ECM). However, best of our knowledge, there no studies on decellularized tumors. Here, we studied biochemical progression ECM two models lung metastases: carcinoma (CAR) melanoma (MEL). We metastatic sections, measured micromechanics ECM, stained sections for proteins, proliferation, cell death markers. The same methodology was applied MEL...
Individuals with atopic dermatitis immunized the small pox vaccine, vaccinia virus (VV), are susceptible to eczema vaccinatum (EV), a potentially fatal disseminated infection. Dysfunction of Forkhead box P3 (FoxP3)-positive regulatory T cells (Treg) has been implicated in pathogenesis dermatitis. To test whether Treg deficiency predisposes EV, we percutaneously VV infected FoxP3-deficient (FoxP3(KO)) mice, which completely lack FoxP3(+) Treg. These animals generated both fewer VV-specific...
: The renin-angiotensin receptor AT2R controls systemic blood pressure and is also suggested to modulate metastasis of cancer cells. However, in the latter case, mechanisms involved downstream remain be defined. We recently described a novel Caveolin-1(CAV1)/Ras-related protein 5A (Rab5)/Ras-related C3 botulinum toxin substrate 1 (Rac1) signaling axis that promotes melanoma, colon, breast Here, we evaluated whether antimetastatic effect connected inhibition this pathway. found murine...
Abstract Caveolin-1 (CAV1) enhanced migration, invasion, and metastasis of cancer cells is inhibited by co-expression the glycoprotein E-cadherin. Although two proteins form a multiprotein complex that includes β-catenin, it remained unclear how this would contribute to blocking promoting function CAV1. Here, we characterized mass spectrometry protein composition CAV1 immunoprecipitates from B16F10 murine melanoma expressing or not The novel tyrosine phosphatase PTPN14 was identified...
Dendritic cell (DC) trafficking from peripheral tissues to lymph nodes is a key step required initiate T responses against pathogens as well tumors. In this context, cellular membrane protrusions and the actin cytoskeleton are essential guide DC migration towards chemotactic signals. Caveolin-1 scaffolding protein that modulates signaling pathways leading remodeling of enhanced cancer cells. However, whether caveolin-1 relevant for function specifically unknown. Here we show expression...
Background: Numerous studies have proposed the use of fluorescent semiconductor nanoparticles or quantum dots (QDs) as novel tools to label cells and tumors. However, QD applications are limited by their toxicity in biological systems little is known about whether QDs affect capacity cancer metastasize. Previously, we described “biomimetic” synthesis CdTe-QDs (QDs-glutathione [GSH]) with increased biocompatibility potential utility labeling cells. Purpose: In order determine feasibility...
Expression of the scaffolding protein Caveolin-1 (CAV1) enhances migration and invasion metastatic cancer cells. Yet, CAV1 also functions as a tumor suppressor in early stages cancer, where expression is suppressed by epigenetic mechanisms. Thus, we sought to identify stimuli/mechanisms that revert silencing cells evaluate how this affects their potential. We reasoned restricted tissue availability anti-neoplastic drugs during chemotherapy might expose sub-therapeutic concentrations, which...
Abstract The fibrotic tumor microenvironment is a pivotal therapeutic target. Nintedanib, clinically approved multikinase antifibrotic inhibitor, effective against lung adenocarcinoma (ADC) but not squamous cell carcinoma (SCC). Previous studies have implicated the secretome of tumor‐associated fibroblasts (TAFs) in selective effects nintedanib ADC, driving factor(s) remained unidentified. Here we examined role tissue inhibitor metalloproteinase‐1 (TIMP‐1), tumor‐promoting cytokine...