A5037033529- Neuroscience and Neuropharmacology Research
- Neuroinflammation and Neurodegeneration Mechanisms
- Plant Stress Responses and Tolerance
- Extracellular vesicles in disease
- Plant nutrient uptake and metabolism
- Weed Control and Herbicide Applications
- Plant responses to water stress
- Amyotrophic Lateral Sclerosis Research
- Genetics and Neurodevelopmental Disorders
- Neurogenesis and neuroplasticity mechanisms
- Rice Cultivation and Yield Improvement
- Molecular Junctions and Nanostructures
- MicroRNA in disease regulation
- Pesticide and Herbicide Environmental Studies
- RNA regulation and disease
- Plant Micronutrient Interactions and Effects
- Neurogenetic and Muscular Disorders Research
- RNA Interference and Gene Delivery
- Allelopathy and phytotoxic interactions
- Advanced biosensing and bioanalysis techniques
- Lipid Membrane Structure and Behavior
- Heavy metals in environment
- RNA Research and Splicing
- Aluminum toxicity and tolerance in plants and animals
- Nerve injury and regeneration
China National Rice Research Institute
2015-2025
Tufts University
2014-2024
Shandong University
1993-2024
Shandong Sport University
2009-2023
Chinese Academy of Agricultural Sciences
2011-2022
Industrial and Commercial Bank of China
2019
Guangxi University
2019
Peking University
2014-2017
Society for Neuroscience
2014
Sichuan University
2012
Perisynaptic astrocytes express important glutamate transporters, especially excitatory amino acid transporter 2 (EAAT2, rodent analog GLT1) to regulate extracellular levels and modulate synaptic activation. In this study, we investigated an exciting new pathway, the exosome-mediated transfer of microRNA (in particular, miR-124a), in neuron-to-astrocyte signaling. Exosomes isolated from neuron-conditioned medium contain abundant microRNAs small RNAs. These exosomes can be directly...
Abstract Astroglia play active and diverse roles in modulating neuronal/synaptic functions the CNS. How these astroglial are regulated, especially by neuronal signals, remains largely unknown. Exosomes, a major type of extracellular vesicles (EVs) that originate from endosomal intraluminal (ILVs), have emerged as new intercellular communication process. By generating cell-type-specific ILVs/exosome reporter (CD63-GFP f/f ) mice immuno-EM/confocal image analysis, we found CD63-GFP + ILVs...
The molecular signature and functional properties of astroglial subtypes in the adult CNS remain largely undefined. By using translational ribosome affinity purification followed by RNA-Seq, we profiled ribosome-associated (presumably translating) mRNAs major cortical subcortical brain regions (cortex, hippocampus, caudate-putamen, nucleus accumbens, thalamus, hypothalamus) BAC <i>aldh1l1</i>-translational (TRAP) mice (both sexes). We found that expression translating closely follows...
Self-assembled monolayers of a series ω-(4'-methyl-biphenyl-4-yl)-alkanethiols (CH3−C6H4−C6H4−(CH2)m−SH, m = 1−6) formed on polycrystalline gold and silver surfaces were characterized in detail by contact angle measurements, optical ellipsometry, X-ray photoelectron spectroscopy (XPS), reflection absorption infrared (IRRAS), near-edge fine structure (NEXAFS). The orientation the biphenyl moiety, determined combining results from IRRAS NEXAFS, exhibits pronounced dependence number methylene...
Abstract Astrocyte heterogeneity remains largely unknown in the CNS due to lack of specific astroglial markers. In this study, molecular identity vivo astrocytes was characterized BAC ALDH1L1 and GLT1 eGFP promoter reporter transgenic mice. is selectively activated adult cortical spinal cord astrocytes, indicated by overlap expression with GFAP, but not NeuN, APC, Olig2, IbaI, PDGFRα immunoreactivity Interestingly, levels (protein, mRNA, activity) were decreased during postnatal maturation....
Diversified neurons are essential for sensorimotor function, but whether astrocytes become specialized to optimize circuit performance remains unclear. Large fast α-motor (FαMNs) of spinal cord innervate fast-twitch muscles that generate peak strength. We report ventral horn express the inward-rectifying K+ channel Kir4.1 (a.k.a. Kcnj10) around MNs in a VGLUT1-dependent manner. Loss astrocyte-encoded selectively altered FαMN size and function led reduced Overexpression was sufficient...
Summary Hydrogen peroxide (H 2 O ) has been reported to increase lignin formation, enhance cell wall rigidification, restrict expansion and inhibit root elongation. However, our results showed that it not only inhibited rice ( Oryza sativa elongation, but also increased diameter. No study how why H increases Exogenous its scavenger 4‐hydroxy‐Tempo were applied confirm the roles of . Immunofluorescence, fluorescence probe, ruthenium red staining, histological section spectrophotometry used...
Abstract Reactive astrocytes (RA) secrete lipocalin-2 (LCN2) glycoprotein that regulates diverse cellular processes including cell death/survival, inflammation, iron delivery and differentiation. Elevated levels of LCN2 are considered as a biomarker brain injury, however, the underlying regulatory mechanisms its expression release not well understood. In this study, we investigated role astrocytic Na + /H exchanger 1 (NHE1) in regulating reactive astrocyte secretion neurodegeneration after...
Recent studies have implicated potentially significant roles for astrocytes in the pathogenesis of neurodevelopmental disorders. Astrocytes undergo a dramatic maturation process following early differentiation from which typical morphology and important functions are acquired. Despite progress understanding their differentiation, very little is known about how become functionally mature. In addition, whether functional disrupted disorders consequences this disruption remains essentially...
Motor neurons are progressively and predominantly degenerated in ALS, which is not only induced by multiple intrinsic pathways but also significantly influenced the neighboring glial cells. In particular, astrocytes derived from SOD1 mutant mouse model of ALS or human familial sporadic patient brain tissue directly induce motor neuron death culture; however, mechanisms pathological astroglial secretion remain unclear. Here we investigated abnormal calcium homeostasis altered exocytosis...
CalliSpheres® Beads (CB) is the first drug-eluting bead (DEB) product in China. Our aim was to compare effect on pharmacokinetics of doxorubicin (DOX) and its local concentration between lipiodol emulsions CB process TACE rabbit livers. Twenty-five rabbits were distributed into two groups; Group 1 received with DOX, 2 loaded DOX (CBDOX). measured peripheral blood at different times after treatment. Livers sampled week month for embolization. distribution liver. The administration by CBDOX...
Fragile X syndrome (FXS) is a neurodevelopmental disorder caused by the loss-of-function of fragile mental retardation protein (FMRP). The loss FMRP function in neurons abolishes its suppression on mGluR1/5-dependent dendritic translation, enhancing synaptic plasticity and other disease phenotypes FXS. In this study, we describe new activation regulating expression astroglial cells. We found that glutamate transporter subtype 1 (GLT1) uptake significantly reduced cortex fmr1(-/-) mice....
In general, the fertility and kernel weight of inferior spikelets rice (Oryza Sativa L.) are obviously lower than those superior spikelets, especially under abiotic stress. However, heat stress at anthesis seems to cause more damage spikelets. When plants were subjected 40℃ anthesis, a greater decrease in was observed compared This decrese has primarily been described their different organ temperatures, which temperature significantly higher that We inferred differences canopy temperature,...
Although, glial cells have well characterized functions in the developing and mature brain, it is only past decade that roles for these behavior plasticity been delineated. Glial astrocytes glia-neuron signaling, example, are now known to important modulatory sleep, circadian behavior, memory plasticity. To better understand mechanisms of signaling context we conducted cell-specific, genome-wide expression profiling adult Drosophila astrocyte-like brain performed RNA interference...
Developing safe and efficient delivery systems for therapeutic biomacromolecules is a long-standing challenge. Herein, we report newly developed combinatorial library of cholesteryl-based disulfide bond-containing biodegradable cationic lipidoid nanoparticles. We have identified subset this which effective protein mRNA in vitro vivo. These lipidoids showed comparable transfection efficacies but much lower cytotoxicities compared to the Lpf2k vitro. In vivo studies adult mice demonstrated...
Abstract Developing astroglia play important roles in regulating synaptogenesis through secreted and contact signals. Whether they regulate postnatal axon growth is unknown. By selectively isolating exosomes using size-exclusion chromatography (SEC) employing cell-type specific exosome reporter mice, our current results define a astroglial pathway that can spread long-range vivo stimulate of cortical pyramidal neurons. Subsequent biochemical genetic studies found surface expression glial...