A5024349927- Salivary Gland Disorders and Functions
- Glycosylation and Glycoproteins Research
- Cancer-related molecular mechanisms research
- Ovarian cancer diagnosis and treatment
- Enzyme-mediated dye degradation
- Endoplasmic Reticulum Stress and Disease
- Galectins and Cancer Biology
- RNA Research and Splicing
- Proteoglycans and glycosaminoglycans research
- Immune cells in cancer
- Genomics, phytochemicals, and oxidative stress
- Salivary Gland Tumors Diagnosis and Treatment
- NF-κB Signaling Pathways
- Epigenetics and DNA Methylation
- Cellular transport and secretion
- Fungal and yeast genetics research
- RNA modifications and cancer
- Reproductive System and Pregnancy
- Diabetes and associated disorders
- Cancer Mechanisms and Therapy
- Fermentation and Sensory Analysis
- Barrier Structure and Function Studies
- Horticultural and Viticultural Research
- Biochemical and biochemical processes
- Bioactive Compounds and Antitumor Agents
University of Chile
2013-2023
Universidad de Ciencias Medicas
2014
Universidad Católica del Norte
2014
Queen's University
2011
Laboratory of Molecular Genetics
2005
Pontificia Universidad Católica de Chile
1995-1999
Austral University of Chile
1993
Disorganization of acinar cell apical microvilli and the presence stromal collagen in lumen suggest that labial salivary gland (LSG) barrier function is impaired patients with Sjögren's syndrome. Tight junctions define polarity regulate paracellular flow ions water, crucial functions cells. This study was undertaken to evaluate expression localization tight junction proteins LSGs from SS determine vitro effects tumor necrosis factor alpha (TNFalpha) interferon-gamma (IFNgamma) on integrity...
Early in cancer development, tumour cells express vascular endothelial growth factor (VEGF), a secreted molecule that is important all stages of angiogenesis, an essential process provides nutrients and oxygen to the nascent tumor thereby enhances tumor-cell survival facilitates growth. Survivin, another protein involved strongly expressed most human cancers, where it promotes by reducing apoptosis as well favoring cell proliferation migration. The mechanisms which induce VEGF expression...
ABSTRACT The ligninolytic system of the basidiomycete Ceriporiopsis subvermispora is composed manganese peroxidase (MnP) and laccase. In this work, source extracellular hydrogen peroxide required for MnP activity was investigated. Our attention focused on possibility that might be generated by itself through oxidation organic acids secreted fungus. Both oxalate glyoxylate were found in fluid C. cultures grown chemically defined media, where also secreted. vivo measured; 14 CO 2 evolution...
Abstract Objective Acinar cells in the salivary glands of patients with Sjögren's syndrome (SS) display severe alterations anchorage to basal lamina. Bioinformatics analysis BP230 gene sequence has revealed presence CpG islands that might be involved epigenetic control expression, and methylation promotor region may implicated as an mechanism gland damage. Thus, present study was undertaken evaluate protein BP230, well proteins BP180, α6β4 integrin, cytokeratin‐18, for their expression...
A hallmark characteristic of SS patients is the ectopic presence mucins MUC5B and MUC7 in extracellular matrix salivary glands that have lost apical-basolateral acinar-cell polarity. This study aims to determine whether exogenous induce gene expression pro-inflammatory cytokines, as well evaluate Toll-like receptor-4 (TLR4) pathway involved this response.Differentiated human submandibular gland (HSG) cells were stimulated with or oligosaccharide residues at different concentrations for...
A total of 11 manganese peroxidase isoenzymes (MnP 1 ‐MnP ) with isoelectric points (pIs) in the range 4.58–3.20 were isolated from liquid‐ and solid‐state cultures basidiomycete Ceriporiopsis subvermispora . In presence hydrogen peroxide, these showed different requirements for Mn(II) oxidation vanillylacetone, o ‐dianisidine, p ‐anisidine ABTS, whereas guaiacol by any isoenzyme did not take place when this metal was omitted. K m values ‐dianisidine absence are 0.5–1.0 mM 4.5–42.0 mM,...
Oral and ocular dryness are frequent serious symptoms of Sjögren's syndrome (SS) that reflect problems in secretion due to glandular dysfunction. Exocytosis, an important process the secretory pathway, requires participation Rab family GTPases. This study was undertaken analyze expression localization Rab3D Rab8A examine their correlation with acinar cell polarity function.Nineteen patients SS 17 controls were evaluated. Levels messenger RNA (mRNA) protein determined by real-time polymerase...
Xerostomia in SS patients has been associated with low quality and quantity of salivary mucins, which are fundamental for the hydration protection oral mucosa. The aim this study was to evaluate if cytokines induce aberrant mucin expression whether tauroursodeoxycholic acid (TUDCA) is able counteract such an anomaly.Labial glands from 16 15 control subjects, as well 3D acini or human submandibular gland cells stimulated TNF-α IFN-γ co-incubated TUDCA, were analysed. mRNA protein levels Mucin...
We have previously shown a functional interaction between human papillomavirus type 16 (HPV-16) E6 and E7 oncoproteins cigarette smoke condensate (CSC) in lung cells suggesting cooperation during carcinogenesis. The molecular mechanisms of such interaction, however, remain to be elucidated. Here we first present evidence showing that has the ability activate HPV-16 p97 promoter by acting on long control region (LCR) epithelial cells. Interestingly, observed CSC-induced activation occurs...
Abstract Ovarian cancer is an aggressive disease of poor prognostic when detected at advanced stage. It widely accepted that the ovarian surface epithelium plays a central role in etiology, but little known about progression molecular level. To identify genes involved tumorigenesis, we carried out genome‐wide transcriptomic analysis six spontaneously transformed mouse epithelial (MOSE) cell lines, vitro model for human carcinoma. Loess normalization followed by statistical with control...
Background: Gastric cancer (GC) ranks as one of the major causes mortality due to worldwide. In Chile, it is currently leading cause death. Identification novel molecular markers that may help improve disease diagnosis at early stages imperative. Materials and Methods: Using whole-genome DNA microarrays we determined differential mRNA levels in fresh human GC samples compared adjacent healthy mucosa from same patients. Genes significantly overexpressed were validated by RT-PCR a group 14...
The progressive decline of the ovarian follicle pool leads to reproductive ageing. latter is accompanied by age-related disorders, including various types cancer. In fact, highest rates cancer (OC) occur at postmenopause while OC risk significantly modulated parity records during previous fertile life. We approached age-parity relationship in C57BL/6 mouse model and herein describe presence nonheme iron (hemosiderin) deposits "age pigment" lipofuscin reproductively aged ovaries applying...
Labial salivary glands (LSGs) of SS patients show alterations related to endoplasmic reticulum stress. Glandular dysfunction could be partly the consequence an altered inositol-requiring enzyme 1α (IRE1α)/X box-binding protein 1 (XBP-1) signalling pathway unfolded response, which then regulates genes involved in biogenesis secretory machinery. This study aimed determine expression, promoter methylation and localization IRE1α/XBP-1 components LSGs also their expression induced by IFN-γ...
MicroRNAs (miRNAs) are small non-coding RNAs (sRNA), that alter gene expression by binding to target messenger (mRNAs) and repressing translation. Dysregulated miRNA has been implicated in the pathogenesis of autoimmune diseases such as Sjögren’s syndrome (SS). The aim this study was characterize global profile sRNAs labial salivary glands (LSG) from SS-patients validate potential candidates glandular inflammation. LSG 21 9 sicca controls were analyzed. A next generation sequencing...
Abstract Skeletal muscle is a highly plastic tissue with remarkable capacity to adapt itself challenges imposed by contractile activity. Adaptive response, that include hypertrophy and activation of oxidative mechanisms have been associated transient changes in transcriptional activity specific genes. To define the set genes regulated depolarizing stimulus, we used 22 K mouse oligonucleotide microarrays. Total RNA from C 2 12 myotubes was obtained at 2, 4, 18, 24 h after high + stimulation....