A5025783077- Ionosphere and magnetosphere dynamics
- Geophysics and Gravity Measurements
- Astrophysics and Cosmic Phenomena
- Light effects on plants
- Fungal and yeast genetics research
- Enzyme-mediated dye degradation
- Circadian rhythm and melatonin
- Microbial Metabolism and Applications
- Photoreceptor and optogenetics research
- Fungal Biology and Applications
- Biofuel production and bioconversion
- Photosynthetic Processes and Mechanisms
- Chromium effects and bioremediation
- Plant Molecular Biology Research
- Methane Hydrates and Related Phenomena
- Mycorrhizal Fungi and Plant Interactions
- Earthquake Detection and Analysis
- Plant and Biological Electrophysiology Studies
- Seismic Waves and Analysis
- Plant-Microbe Interactions and Immunity
- Genomics and Phylogenetic Studies
- Fermentation and Sensory Analysis
- Plant Pathogens and Fungal Diseases
- Gene Regulatory Network Analysis
- Microbial Metabolic Engineering and Bioproduction
Pontificia Universidad Católica de Chile
2016-2025
Millennium Science Initiative
2020-2025
Millennium Institute for Integrative Biology
2012-2025
Agencia Nacional de Investigación y Desarrollo
2023
Dartmouth College
2006-2015
Dartmouth Hospital
2006
Madison Group (United States)
2004
Joint Genome Institute
2004
Universidad Andrés Bello
2001
Wood is a major pool of organic carbon that highly resistant to decay, owing largely the presence lignin. The only organisms capable substantial lignin decay are white rot fungi in Agaricomycetes, which also contains non-lignin-degrading brown and ectomycorrhizal species. Comparative analyses 31 fungal genomes (12 generated for this study) suggest lignin-degrading peroxidases expanded lineage leading ancestor reconstructed as species, then contracted parallel lineages mycorrhizal Molecular...
Trichoderma reesei is the main industrial source of cellulases and hemicellulases used to depolymerize biomass simple sugars that are converted chemical intermediates biofuels, such as ethanol. We assembled 89 scaffolds (sets ordered oriented contigs) generate 34 Mbp nearly contiguous T. genome sequence comprising 9,129 predicted gene models. Unexpectedly, considering utility effectiveness carbohydrate-active enzymes reesei, its encodes fewer than any other sequenced fungus able hydrolyze...
White rot fungi efficiently degrade lignin, a complex aromatic polymer in wood that is among the most abundant natural materials on earth. These use extracellular oxidative enzymes are also able to transform related compounds found explosive contaminants, pesticides and toxic waste. We have sequenced 30-million base-pair genome of Phanerochaete chrysosporium strain RP78 using whole shotgun approach. The P. reveals an impressive array genes encoding secreted oxidases, peroxidases hydrolytic...
Brown-rot fungi such as Postia placenta are common inhabitants of forest ecosystems and also largely responsible for the destructive decay wooden structures. Rapid depolymerization cellulose is a distinguishing feature brown-rot, but biochemical mechanisms underlying genetics poorly understood. Systematic examination P. genome, transcriptome, secretome revealed unique extracellular enzyme systems, including an unusual repertoire glycoside hydrolases. Genes encoding exocellobiohydrolases...
Efficient lignin depolymerization is unique to the wood decay basidiomycetes, collectively referred as white rot fungi. Phanerochaete chrysosporium simultaneously degrades and cellulose, whereas closely related species, Ceriporiopsis subvermispora, also depolymerizes but may do so with relatively little cellulose degradation. To investigate basis for selective ligninolysis, we conducted comparative genome analysis of C. subvermispora P. . Genes encoding manganese peroxidase numbered 13 five...
band , an allele enabling clear visualization of circadianly regulated spore formation (conidial banding), has remained integral tool in the study circadian rhythms for 40 years. bd was mapped using single-nucleotide polymorphisms (SNPs), cloned, and determined to be a T79I point mutation ras-1 . Alterations light-regulated gene expression mutant suggests that Neurospora photoreceptor WHITE COLLAR-1 is target RAS signaling, increases transcription both wc-1 fluffy show regulators conidiation...
The mechanistic basis of eukaryotic circadian oscillators in model systems as diverse Neurospora, Drosophila, and mammalian cells is thought to be a transcription-and-translation-based negative feedback loop, wherein progressive controlled phosphorylation one or more elements ultimately elicits their own proteasome-mediated degradation, thereby releasing determining period length. Neurospora crassa element FREQUENCY (FRQ) exemplifies such proteins; it progressively phosphorylated at than 100...
Organisms are exposed to a tough environment, where acute daily challenges, like light, can strongly affect several aspects of an individual's physiology, including pathogenesis. While fungal models have been widely employed understand the physiological and molecular events associated with light perception, various other agricultural-relevant fungi still remain, in terms their responsiveness dark. The fungus Botrytis cinerea is aggressive pathogen able cause disease on wide range plant...
Significance Circadian clocks are molecular machineries that allow organisms to anticipate daily cyclic challenges and temporally modulate different processes. Thus, plant defense mechanisms against pathogens have been reported vary in Arabidopsis thaliana . Although the plant–pathogen interaction is a two-sided story, nothing known regarding circadian regulation of pathogenic traits. Herein we characterize functional clock necrotrophic fungal pathogen Botrytis cinerea , postulating...
Collectively classified as white-rot fungi, certain basidiomycetes efficiently degrade the major structural polymers of wood cell walls. A small subset these Agaricomycetes, exemplified by Phlebiopsis gigantea, is capable colonizing freshly exposed conifer sapwood despite its high content extractives, which retards establishment other fungal species. The mechanism(s) P. gigantea tolerates and metabolizes resinous compounds have not been explored. Here, we report annotated genome compare...
We report the complete reconstruction of firefly luciferase gene, fully codon optimized for expression in Neurospora crassa. This reporter enhances light output by approximately 4 log orders over that with previously available versions, now producing is visible to naked eye and sufficient monitoring activities many poorly expressed genes. Time lapse photography strains growing race tubes, which frq or eas/ccg-2 promoter used drive luciferase, shows highest levels activity near growth front...
Significance Circadian rhythms provide temporal information to other cellular processes, such as metabolism. We investigate the coupling between cell cycle and circadian clock using mathematical modeling experimentally validate model-driven predictions with a model filamentous fungus, Neurospora crassa . demonstrate conserved mechanism in mammals, which results clock-gated mitotic cycles. Furthermore, we observe clock-dependent phase shifts of G1 G2 cyclins, may alter timing divisions. Our...
Lignin degradation by the white rot basidiomycete Phanerochaete chrysosporium involves various extracellular oxidative enzymes, including lignin peroxidase, manganese and a peroxide-generating enzyme, glyoxal oxidase. Recent studies have suggested that laccases also may be produced this fungus, but these conclusions been controversial. We identified four sequences related to ferroxidases (Fet3) in search of publicly available P. database. One gene, designated mco1, has typical eukaryotic...
Population-level sampling and whole-genome sequences of different individuals allow one to identify signatures hybridization, gene flow potential molecular mechanisms environmental responses. Here, we report the isolation 160 Saccharomyces eubayanus strains, cryotolerant ancestor lager yeast, from ten sites in Patagonia along 2,000 km Nothofagus forests. Frequency S. isolates was higher towards southern colder regions, demonstrating nature species. We sequenced genome 82 strains and,...
Sequential sugar consumption, from a preferred source to less one, represents critical metabolic adaptation in yeast, which is particularly relevant for survival fluctuating environments such as those found beer fermentation. However, transitions are an environmental variable that challenging predict and detect, impacting the outcome of fermentations. This protocol describes vivo system monitor transcriptional activation associated with glucose-to-maltose shift Saccharomyces eubayanus...
Abstract Linkage mapping studies in model organisms have typically focused their efforts polymorphisms within coding regions, ignoring those regulatory regions that may contribute to gene expression variation. In this context, differences transcript abundance are frequently proposed as a source of phenotypic diversity between individuals, however, until now, little molecular evidence has been provided. Here, we examined Allele Specific Expression (ASE) six F1 hybrids from Saccharomyces...