A5025014292- Fermentation and Sensory Analysis
- Fungal and yeast genetics research
- Horticultural and Viticultural Research
- Yeasts and Rust Fungi Studies
- Genetic Mapping and Diversity in Plants and Animals
- Microbial Metabolic Engineering and Bioproduction
- SARS-CoV-2 detection and testing
- Bioinformatics and Genomic Networks
- Wine Industry and Tourism
- Phytochemicals and Antioxidant Activities
- Evolution and Genetic Dynamics
- Advanced Chemical Sensor Technologies
- Plant Pathogens and Fungal Diseases
- COVID-19 diagnosis using AI
- Plant Molecular Biology Research
- Genomics and Phylogenetic Studies
- Mycorrhizal Fungi and Plant Interactions
- Thermochemical Biomass Conversion Processes
- Microbial Natural Products and Biosynthesis
- Fungal Biology and Applications
- Lignin and Wood Chemistry
- Evolutionary Algorithms and Applications
- Biosensors and Analytical Detection
- Biochemical Analysis and Sensing Techniques
- SARS-CoV-2 and COVID-19 Research
Universidad de Santiago de Chile
2016-2025
Millennium Science Initiative
2020-2025
Millennium Institute for Integrative Biology
2017-2025
Millennium Nucleus of Ion Channel Associated Diseases
2022-2025
Agencia Nacional de Investigación y Desarrollo
2021
Centro de Estudios Científicos
2018
Pontificia Universidad Católica de Chile
2016-2017
Millennium Engineering and Integration (United States)
2016
AgroParisTech
2012-2014
Institut Jean-Pierre Bourgin
2012-2014
A fundamental goal in biology is to achieve a mechanistic understanding of how and what extent ecological variation imposes selection for distinct traits favors the fixation specific genetic variants. Key such an detailed mapping natural genomic phenomic space bridging gap that separates these worlds. Here we chart high-resolution map trait one most important model organisms, budding yeast Saccharomyces cerevisiae, its closest wild relatives trace basis timing major phenotype changing events...
One approach to understanding the genetic basis of traits is study their pattern inheritance among offspring phenotypically different parents. Previously, such analysis has been limited by low mapping resolution, high labor costs, and large sample size requirements for detecting modest effects. Here, we present a novel map trait loci using artificial selection. First, generated populations 10–100 million haploid diploid segregants crossing two budding yeast strains heat tolerance up 12...
Abstract Phenotypic variation arising from populations adapting to different niches has a complex underlying genetic architecture. A major challenge in modern biology is identify the causative variants driving phenotypic variation. Recently, baker’s yeast, Saccharomyces cerevisiae emerged as powerful model for dissecting traits. However, past studies using laboratory strain were unable reveal complete architecture of polygenic Here, we present linkage study 576 recombinant strains obtained...
A large fraction of human complex trait heritability is due to a high number variants with small marginal effects and their interactions genotype environment. Such alleles are more easily studied in model organisms, where environment, genetic makeup, allele frequencies can be controlled. Here, we examine the effect natural variation on heritable traits very pool baker's yeast from multiparent 12th generation intercross. We selected four representative founder strains produce Saccharomyces...
Saccharomyces cerevisiae has proved to be an invaluable model in classical and molecular genetics studies. Despite several hundreds of isolates already available, the scientific community relies on use only a handful unrelated strains. The lack sequence information, haploid derivatives genetic markers prevented novel strains from being used. Here, we release set 55 S. paradoxus genetically tractable strains, previously sequenced Genome Resequencing Project. These are stable ura3 auxotrophs...
Yeasts are ubiquitous in temperate forests. While this broad habitat is well-defined, the yeasts inhabiting it and their life cycles, niches, contributions to ecosystem functioning less understood. present on nearly all sampled substrates forests worldwide. They associate with soils, macroorganisms, other habitats no doubt contribute broader ecosystem-wide processes. Researchers have gathered information leading hypotheses about yeasts' niches cycles based physiological observations...
Lager yeasts are limited to a few strains worldwide, imposing restrictions on flavour and aroma diversity hindering our understanding of the complex evolutionary mechanisms during yeast domestication. The recent finding diverse S . eubayanus lineages from Patagonia offers potential for generating new lager with different profiles. Here, we leverage natural genetic expand repertoire by including three distinct Patagonian lineages. We used experimental evolution selection desirable traits...
Comparative genomics is a formidable tool to identify functional elements throughout genome. In the past ten years, studies in budding yeast Saccharomyces cerevisiae and set of closely related species have been instrumental showing benefit analyzing patterns sequence conservation. Increasing number genome sequences makes comparative approach more powerful accurate. Here, we report analysis arboricolus, recently isolated China, that S. cerevisiae. We obtained high quality de novo assemblies...
A fundamental question in biology is whether variation organisms primarily emerges as a function of adaptation or neutral genetic drift. Trait the model organism baker's yeast follows population bottlenecks rather than environmental boundaries suggesting that it results from Based on life history, we hypothesized population-specific loss-of-function mutations emerging genes recently released selection predominant cause trait within species. As retention one functional copy gene diploid...
cis- and trans-acting factors affect gene expression responses to environmental conditions. However, for most plant systems, we lack a comprehensive map of these their interaction with variation. Here, examined allele-specific (ASE) in an F1 hybrid study how alleles from two Arabidopsis thaliana accessions expression. To investigate the effect environment, used drought stress developed variance component model estimate combined genetic contributions trans-regulatory polymorphisms, factors,...
Saccharomyces cerevisiae is the main microorganism responsible for wine alcoholic fermentation. The oenological phenotypes resulting from fermentation, such as production of acetic acid, glycerol, and residual sugar concentration are regulated by multiple genes vary quantitatively between different strain backgrounds. With aim identifying quantitative trait loci (QTLs) that regulate phenotypes, we performed linkage analysis using three crosses highly diverged S. strains. Segregants each...
Population-level sampling and whole-genome sequences of different individuals allow one to identify signatures hybridization, gene flow potential molecular mechanisms environmental responses. Here, we report the isolation 160 Saccharomyces eubayanus strains, cryotolerant ancestor lager yeast, from ten sites in Patagonia along 2,000 km Nothofagus forests. Frequency S. isolates was higher towards southern colder regions, demonstrating nature species. We sequenced genome 82 strains and,...
Saccharomyces cerevisiae strains are genetically diverse, largely as a result of human efforts to develop specifically adapted various fermentation processes. These adaptive pressures from ecological niches have generated behavioral differences among these strains, particularly in terms their nitrogen consumption capacities. In this work, we characterize phenotype by the specific quantity consumed under oenological conditions using new approach. Indeed, unlike previous studies, our...
Abstract Saccharomyces cerevisiae is responsible for wine must fermentation. In this process, nitrogen represents a limiting nutrient and its scarcity results in important economic losses the industry. Yeast isolates use different strategies to grow poor environments their genomic plasticity enables adaptation multiple habitats through improvements consumption. Here, we used highly recombinant S. multi-parent population (SGRP-4X) derived from intercross of four parental strains origins...
Different populations within a species represent rich reservoir of allelic variants, corresponding to an evolutionary signature withstood environmental constraints. Saccharomyces cerevisiae strains are widely utilised in the fermentation different kinds alcoholic beverages, such as, wine and sake, each them derived from must with distinct nutrient composition. Importantly, adequate nitrogen levels medium essential for process, however, comprehensive understanding genetic variants determining...
Abstract Linkage mapping studies in model organisms have typically focused their efforts polymorphisms within coding regions, ignoring those regulatory regions that may contribute to gene expression variation. In this context, differences transcript abundance are frequently proposed as a source of phenotypic diversity between individuals, however, until now, little molecular evidence has been provided. Here, we examined Allele Specific Expression (ASE) six F1 hybrids from Saccharomyces...
The COVID-19 pandemic has resulted in millions of deaths globally, and while several diagnostic systems were proposed, real-time reverse transcription polymerase chain reaction (RT-PCR) remains the gold standard. However, reagents, including enzymes used RT-PCR, are subject to centralized production models intellectual property restrictions, which present a challenge for less developed countries. With aim generating standardized One-Step open RT-qPCR protocol detect SARS-CoV-2 RNA clinical...
<title>Abstract</title> The nature and formation history of our Galaxy's largest most enigmatic stellar cluster, known as Omega Centauri (oCen) remains debated. Here, we offer a novel approach to disentangling the complex populations within oCen based on phylogenetics methodologies from evolutionary biology. These include Gaussian Mixture Model Neighbor-Joining clustering algorithms applied set chemical abundances members. Instead using classical in astronomy grouping them into separate...
Abstract The rhizosphere is the soil region around plant roots hosting a diverse microbial community, influencing nutrient availability and how plants react to extreme conditions. However, our understanding of fungi biodiversity impact environmental variations on this still in its infancy. Our study investigates fungal communities’ diversity functional traits Nothofagus pumilio, one few winters deciduous treeline species world, forming southern South America. At four distinct locations...