A5022044396- Fungal and yeast genetics research
- Bioinformatics and Genomic Networks
- Evolution and Genetic Dynamics
- Fermentation and Sensory Analysis
- RNA and protein synthesis mechanisms
- Cavitation Phenomena in Pumps
- Water Systems and Optimization
- Microbial Metabolic Engineering and Bioproduction
- Hydraulic and Pneumatic Systems
- Genomics and Phylogenetic Studies
- CRISPR and Genetic Engineering
- Yeasts and Rust Fungi Studies
- Chromosomal and Genetic Variations
- Protein Structure and Dynamics
- Plant Disease Resistance and Genetics
- Biofuel production and bioconversion
- Parasite Biology and Host Interactions
- Gene Regulatory Network Analysis
- Genetic Mapping and Diversity in Plants and Animals
- Antifungal resistance and susceptibility
- Genetic diversity and population structure
- Horticultural and Viticultural Research
- Microtubule and mitosis dynamics
- Genomics and Chromatin Dynamics
- RNA Research and Splicing
Université Laval
2016-2025
PROTEO
2016-2025
Département d'Informatique
2019-2025
Université du Québec à Montréal
2023-2024
Vision Engineering (Italy)
2017-2023
Keio University Shonan Fujisawa
2023
The University of Sydney
2023
Yale University
2023
Mitchell Institute
2023
Texas A&M University
2023
Protein interactions regulate the systems-level behavior of cells; thus, deciphering structure and dynamics protein interaction networks in their cellular context is a central goal biology. We have performed genome-wide vivo screen for protein-protein Saccharomyces cerevisiae by means protein-fragment complementation assay (PCA). identified 2770 among 1124 endogenously expressed proteins. Comparison with previous studies confirmed known interactions, but most were not known, revealing...
The mutation process ultimately defines the genetic features of all populations and, hence, has a bearing on wide range issues involving evolutionary genetics, inheritance, and disorders, including predisposition to cancer. Nevertheless, formidable technical barriers have constrained our understanding rate at which mutations arise molecular spectrum their effects. Here, we report use complete-genome sequencing in characterization spontaneously arising yeast Saccharomyces cerevisiae. Our...
Identifying the properties of gene networks that influence their evolution is a fundamental research goal. However, modes cannot be inferred solely from distribution natural variation, because selection interacts with demography and mutation rates to shape polymorphism divergence. We estimated effects naturally occurring mutations on expression while minimizing effect selection. demonstrate sensitivity increases both increasing trans-mutational target size presence TATA box. Genes greater...
Previous studies in Saccharomyces cerevisiae have demonstrated that cryptic promoters within coding regions activate transcription particular mutants. We performed a comprehensive analysis of order to identify factors normally repress promoters, determine the amount genome-wide, and study potential for expression genetic information by transcription. Our results show large number control chromatin structure are required from at least 1,000 locations across S. genome. Two suggest some...
According to the theory of mate choice based on heterozygosity, mates should choose each other in order increase heterozygosity their offspring. In this study, we tested 'good genes as heterozygosity' hypothesis by documenting mating patterns wild Atlantic salmon (Salmo salar) using both major histocompatibility complex (MHC) and microsatellite loci. Specifically, null hypotheses that is not dependent relatedness between potential partners or MHC similarity mates. Three parameters were...
Hybrids between species are often characterized by novel gene-expression patterns. A recent study on allele-specific gene expression in hybrids of Drosophila revealed cases which cis- and trans-regulatory elements within had coevolved such a way that changes cis-regulatory compensated elements. We hypothesized coevolution should lead to misexpression the hybrid. To test this hypothesis, we estimated overall levels for 31 genes D. melanogaster, simulans, their F1 found 13 with cis-trans...
Atlantic salmon ( Salmo salar ) undergo spectacular marine migrations before homing to spawn in natal rivers. However, males that grow fastest early life can adopt an alternative ‘sneaker’ tactic by maturing earlier at greatly reduced size without leaving freshwater. While the ultimate evolutionary causes have been well studied, virtually nothing is known about molecular bases of this developmental plasticity. We investigate nature and extent coordinated changes accompany such a fundamental...
Abstract Evidence of selection acting on major histocompatibility complex (MHC) genes has been illustrated with the analysis their nucleotide sequences and allele frequency distribution. Comparing patterns population differentiation at neutral markers MHC in wild may provide further insights about relative role neutrality shaping diversity. In this study, we combine both methods to assess a gene Atlantic salmon. We compare variation class II B locus microsatellites among 14 samples from...
The G protein-coupled receptor (GPCR) superfamily represents the most important class of pharmaceutical targets. Therefore, characterization cascades and their ligands is a prerequisite to discovering novel drugs. Quantification agonist-induced second messengers downstream-coupled kinase activities central GPCRs or other pathways that converge on GPCR-mediated signaling. Furthermore, there need for simple, cell-based assays would report direct indirect actions effectors More generally,...
Lager-style beers constitute the vast majority of beer market, and yet, genetic origin yeast strains that brew them has been shrouded in mystery controversy. Unlike ale-style beers, which are generally brewed with Saccharomyces cerevisiae, lagers at colder temperatures allopolyploid hybrids eubayanus x S. cerevisiae. Since discovery 2011, additional have isolated from South America, North Australasia, Asia, but only interspecies Europe. Here, using genome sequence data, we examine...
Robustness of protein networks It is thought that gene duplication helps cells maintain genetic robustness, but this seems not to be the whole story. Diss et al. investigated fate protein-protein interactions among duplicated genes in yeast. Some interacting duplicates evolved mutual dependence, resulting a more fragile system. This finding us understand evolutionary trajectories duplications and how seemingly redundant can increase complexity interaction networks. Science , issue p. 630
Recent functional, proteomic and ribosome profiling studies in eukaryotes have concurrently demonstrated the translation of alternative open-reading frames (altORFs) addition to annotated protein coding sequences (CDSs). We show that a large number small proteins could fact be coded by these altORFs. The putative translated from altORFs orthologs many species contain functional domains. Evolutionary analyses indicate often more extreme conservation patterns than their CDSs. Thousands are...
Reproductive isolation is a critical step in the process of speciation. Among most important factors driving reproductive are genetic incompatibilities. Whether these incompatibilities already present before extrinsic prevent gene flow between incipient species remains largely unresolved natural systems. This question particularly challenging because it requires that we catch speciating populations act they reach full-fledged status. We measured extent intrinsic postzygotic within and...
Abstract Hybridization can result in reproductively isolated and phenotypically distinct lineages that evolve as independent hybrid species. How frequently hybridization leads to speciation remains largely unknown. Here we examine the potential recurrence of wild yeast Saccharomyces paradoxus North America, which comprises two endemic SpB SpC , an incipient species, *. Using whole-genome sequences from more than 300 strains, uncover origin another group, SpD emerged between * one its...