A5044351054- Fungal and yeast genetics research
- Bioinformatics and Genomic Networks
- RNA and protein synthesis mechanisms
- Biofuel production and bioconversion
- Protein Structure and Dynamics
- Protist diversity and phylogeny
- Evolution and Genetic Dynamics
- CRISPR and Genetic Engineering
- Genomics and Phylogenetic Studies
- Microbial Metabolic Engineering and Bioproduction
- Genetic Mapping and Diversity in Plants and Animals
- Transgenic Plants and Applications
- Marine Biology and Ecology Research
- Animal Genetics and Reproduction
- Genomics and Chromatin Dynamics
- Enzyme Structure and Function
- Ubiquitin and proteasome pathways
- Chromosomal and Genetic Variations
- Microtubule and mitosis dynamics
- Microbial Community Ecology and Physiology
Université Laval
2017-2022
PROTEO
2017-2022
Instituto Politécnico Nacional
2013-2022
Instituto de Estudios Avanzados
2020
Center for Research and Advanced Studies of the National Polytechnic Institute
2013-2017
Robustness of protein networks It is thought that gene duplication helps cells maintain genetic robustness, but this seems not to be the whole story. Diss et al. investigated fate protein-protein interactions among duplicated genes in yeast. Some interacting duplicates evolved mutual dependence, resulting a more fragile system. This finding us understand evolutionary trajectories duplications and how seemingly redundant can increase complexity interaction networks. Science , issue p. 630
Significance Many studies have focused on the mechanisms of long-term retention gene duplicates, such as gain functions or reciprocal losses. However, changes are more likely to occur if duplicates maintained for a long period. This time span will be short duplication is immediately deleterious. We measured distribution fitness effects 899 genes in budding yeast. find that deleterious than beneficial. contrary previous models, general, does not affect by altering organization protein...
Gene duplication is a driver of the evolution new functions. The genes encoding homomeric proteins leads to formation homomers and heteromers paralogs, creating complexes after single event. loss these may be required for two paralogs evolve independent Using yeast as model, we find that heteromerization frequent among duplicated correlates with functional similarity between paralogs. in silico evolution, show sharing binding interfaces, mutations one paralog can have structural pleiotropic...
A single gene can partake in several biological processes, and therefore deletions lead to different-sometimes unexpected-phenotypes. However, it is not always clear whether such pleiotropy reflects the loss of a unique molecular activity involved different processes or multifunctional protein. Here, using Saccharomyces cerevisiae metabolism as model, we systematically test null hypothesis that enzyme phenotypes depend on annotated function, namely their catalysis. We screened set carefully...
Whole-genome duplication (WGD) events have shaped the genomes of eukaryotic organisms. Relaxed selection after along with inherent functional constraints are thought to determine fate paralogs and, ultimately, evolution gene function. Here, we investigated rate protein (as measured by dN/dS ratios) before and WGD in hemiascomycete yeasts, way which changes such rates relate molecular biological For most groups orthologous genes (81%) observed a change genome duplication. Genes atypically-low...
Barcode fusion genetics (BFG) utilizes deep sequencing to improve the throughput of protein-protein interaction (PPI) screening in pools. BFG has been implemented Yeast two-hybrid (Y2H) screens (BFG-Y2H). While Y2H requires test protein pairs localize nucleus for reporter reconstruction, dihydrofolate reductase protein-fragment complementation assay (DHFR-PCA) allows proteins broader subcellular contexts and proves be largely orthogonal Y2H. Here, we DHFR-PCA (BFG-PCA). This plasmid-based...
Abstract Many paralogs derive from the duplication of genes encoding homomeric proteins. Such events lead to formation homomers and heteromers, thus creating new structures a single event. We exhaustively characterize this phenomenon using budding yeast protein-protein interaction network. observe that heteromerize are very frequent less functionally diverged than those lost property, raising possibility heteromerization prevents functional divergence. Using in silico evolution, we show for...
ABSTRACT A single gene can partake in several biological processes, and therefore deletions lead to different—sometimes unexpected—phenotypes. However, it is not always clear whether such pleiotropy reflects the loss of a unique molecular activity involved different processes or multifunctional protein. Here, using Saccharomyces cerevisiae metabolism as model, we systematically test null hypothesis that enzyme phenotypes depend on annotated function, namely their catalysis. We screened set...
Abstract Gene duplication is ubiquitous and a major driver of phenotypic diversity across the tree life, but its immediate consequences are not fully understood. Deleterious effects would decrease probability retention duplicates prevent their contribution to long term evolution. One possible detrimental effect perturbation stoichiometry protein complexes. Here, we measured fitness 899 essential genes in budding yeast using high-resolution competition assays. At least ten percent caused...
ABSTRACT Barcode fusion genetics (BFG) utilizes deep sequencing to improve the throughput of protein-protein interaction (PPI) screening in pools. BFG has been implemented Yeast two-hybrid (Y2H) screens (BFG-Y2H). While Y2H requires test protein pairs localize nucleus for reporter reconstruction, Dihydrofolate Reductase Protein-Fragment Complementation Assay (DHFR-PCA) allows proteins broader subcellular contexts and proves be largely orthogonal Y2H. Here, we DHFR-PCA (BFG-PCA). This...