A5076288524- Circadian rhythm and melatonin
- Biofuel production and bioconversion
- Light effects on plants
- Microbial Metabolic Engineering and Bioproduction
- Photoreceptor and optogenetics research
- Fungal and yeast genetics research
- Genetics, Aging, and Longevity in Model Organisms
- Plant and Biological Electrophysiology Studies
- Algal biology and biofuel production
- Avian ecology and behavior
- Fermentation and Sensory Analysis
- Spaceflight effects on biology
- Lipid Membrane Structure and Behavior
- Plant Molecular Biology Research
- Glycosylation and Glycoproteins Research
- Studies on Chitinases and Chitosanases
- Carbohydrate Chemistry and Synthesis
- Catalysis for Biomass Conversion
- Anaerobic Digestion and Biogas Production
- Marine and coastal plant biology
- Autism Spectrum Disorder Research
- Marine and fisheries research
- Metalloenzymes and iron-sulfur proteins
- Biodiesel Production and Applications
- Advanced Neuroimaging Techniques and Applications
Masco (United States)
2015-2022
Massachusetts Institute of Technology
2016
McGovern Institute for Brain Research
2016
Howard Hughes Medical Institute
2016
Bioenergy Life Science (United States)
2013
Dartmouth College
2002-2010
University of Utah
2010
Imperial College London
1999
University of Virginia
1995
In the fungus Neurospora crassa , blue light photoreceptor(s) and signaling pathway(s) have not been identified. We examined by exploiting sensitivity of biological clock, specifically rapid induction clock component frequency ( frq ). Light is transcriptionally controlled requires two cis-acting elements (LREs) in promoter. Both LREs are bound a White Collar–1 (WC-1)/White Collar–2 (WC-2)–containing complex (WCC), causes decreased mobility WCC to LREs. The use vitro–translated WC-1 WC-2...
The cortical underconnectivity theory asserts that reduced long-range functional connectivity might contribute to a neural mechanism for autism. We examined resting-state blood oxygen level–dependent interhemispheric correlation in 53 males with high-functioning autism and 39 typically developing from late childhood through early adulthood. By constructing spatial maps of between homologous voxels each hemisphere, we found significantly specific regions relevance autism: sensorimotor cortex,...
The biological clock of Neurospora crassa includes interconnected transcriptional and translational feedback loops that cause both the transcript protein encoded by frequency gene ( frq ) to undergo robust daily oscillations in abundance, which are essential for function. To understand better mechanism generating rhythmic transcript, reporter constructs were used show oscillation message is transcriptionally regulated, a single cis-acting element promoter, Clock Box (C box), necessary...
band , an allele enabling clear visualization of circadianly regulated spore formation (conidial banding), has remained integral tool in the study circadian rhythms for 40 years. bd was mapped using single-nucleotide polymorphisms (SNPs), cloned, and determined to be a T79I point mutation ras-1 . Alterations light-regulated gene expression mutant suggests that Neurospora photoreceptor WHITE COLLAR-1 is target RAS signaling, increases transcription both wc-1 fluffy show regulators conidiation...
The main technological impediment to widespread utilization of lignocellulose for the production fuels and chemicals is lack low-cost technologies overcome its recalcitrance. Organisms that hydrolyze produce a valuable product such as ethanol at high rate titer could significantly reduce costs biomass conversion technologies, will allow separate steps be combined in consolidated bioprocess (CBP). Development Saccharomyces cerevisiae CBP requires level secretion cellulases, particularly...
ABSTRACT Phytochromes (Phys) comprise a superfamily of red-/far-red-light-sensing proteins. Whereas higher-plant Phys that control numerous growth and developmental processes have been well described, the biochemical characteristics functions microbial forms are largely unknown. Here, we describe analyses expression, regulation, activities two in filamentous fungus Neurospora crassa . In addition to containing signature N-terminal domain predicted covalently associate with bilin chromophore,...
In plants and animals, cryptochromes function as either photoreceptors or circadian clock components. We have examined the cryptochrome from filamentous fungus Neurospora crassa demonstrate that cry encodes a DASH-type appears capable of binding flavin adenine dinucleotide (FAD) methenyltetrahydrofolate (MTHF). The transcript CRY protein levels are strongly induced by blue light in wc-1-dependent manner, is circadianly regulated, with peak abundance opposite phase to frq. Neither deletion...
Abstract Producing second‐generation ethanol from the five carbon ( C5 ) sugars in bagasse and cane trash could increase yield at first‐generation sugarcane biorefineries Brazil. Co‐fermenting with juice molasses fermenters is a potentially attractive process configuration enabled by recent biotechnology advances. To assess feasibility of this cofermentation, batch fermentations supplemented xylose, primary sugar hemicellulose, were conducted lab scale using yeast strain, M3799 , engineered...
Saccharomyces cerevisiae has recently been engineered to use acetate, a primary inhibitor in lignocellulosic hydrolysates, as cosubstrate during anaerobic ethanolic fermentation. However, the original metabolic pathway devised convert acetate ethanol uses NADH-specific acetylating acetaldehyde dehydrogenase and alcohol quickly becomes constrained by limited NADH availability, even when glycerol formation is abolished. We present novel target for redox engineering of S. cerevisiae....
ABSTRACT Saccharomyces cerevisiae strain M3707 was isolated from a sample of commercial distillers yeast, and its genome sequence together with the sequences for four derived haploid strains M3836, M3837, M3838, M3839 has been determined. Yeasts have potential consolidated bioprocessing (CBP) biofuel production, access to these will facilitate their development.
In Neurosporacrassa the FRQ/WC feedback loop has been shown to be central function of circadian clock. Similar other eukaryotic systems it is based on a transcription–translation PAS heterodimer type feedback. FRQ levels cycle with period identical that Neurospora and its expression rapidly induced by light. A complex White Collar 1 (WC-1) 2 (WC-2) (the WCC) required for transcriptional activation frq. The oscillation in frq message transcriptionally regulated via single necessary sufficient...
During the 2006/07 Antarctic summer, species population, distribution and reproductive behavior of penguins in areas near Great Wall Station were investigated. Five penguin recorded: gentoo (Pygoscelis papua ), adelie (P. adeliae chinstrap ( P. antarctica King Aptenodytes patagonicus ) Emperor A. forsteri ). The first three bred locally, while other two observed occasionally. Ardley Island is one most important breeding for penguins. After season 2006/07, there a total about 17 234 success...
During the period of 2006 /07 Antarctic summ er, species population, distribution and re2 p roductive behavior penguins in areas Great W all Station were investigated.Five penguin recorded, Gentoo ( P. papua) , Adelie Py2goscelis adeliae) Chinstrap an ta rctica ) King (A ptenody tes pa tagon i2 cus) Emperor (A.forsteri).The first three bred locally, while other t wo m et occasionally.A rdley Island was one most i mportant breeding for penguins, after season /2007, there totally about 17234 w...
ABSTRACT Both hexose- and pentose-fermenting yeasts are commercially available. The aim of this study was to test several yeast strains for their ability ferment lignocellulosic feedstock evaluate usability in a bioethanol production process based on the Cellunolix ® concept. demonstration plant St1 uses sawdust produce second-generation ethanol. performed collaboration with providers using two types pretreated filtered ethanol hydrolysate originating from pine willow. Ten pentose- three...