A5048599897- Gold and Silver Nanoparticles Synthesis and Applications
- Nanoparticle-Based Drug Delivery
- Nanocluster Synthesis and Applications
- Advanced biosensing and bioanalysis techniques
- Pesticide and Herbicide Environmental Studies
- Alzheimer's disease research and treatments
- RNA Interference and Gene Delivery
- Weed Control and Herbicide Applications
- Nanoplatforms for cancer theranostics
- Advanced Drug Delivery Systems
- Nanoparticles: synthesis and applications
- Supramolecular Self-Assembly in Materials
- Chemical Synthesis and Analysis
- Plant and soil sciences
- Advanced Nanomaterials in Catalysis
- Plant tissue culture and regeneration
- Pharmaceutical and Antibiotic Environmental Impacts
- Electrochemical sensors and biosensors
- Graphene and Nanomaterials Applications
- Porphyrin and Phthalocyanine Chemistry
- Neonatal Health and Biochemistry
- Quantum Dots Synthesis And Properties
- Barrier Structure and Function Studies
- Protein Interaction Studies and Fluorescence Analysis
- Cholinesterase and Neurodegenerative Diseases
University of Chile
2016-2025
Advanced Center for Chronic Diseases
2016-2025
M. Kumarasamy College of Engineering
2025
Mandya Institute of Medical Sciences
2024
University of Cartagena
2024
Pontificia Universidad Católica de Chile
2007-2023
Universitat de Barcelona
2001-2022
Institute for Bioengineering of Catalonia
2008-2022
Instituto de Ciências Farmacêuticas
2008-2021
Centro de Recursos Educativos Avanzados
2021
The lack of predictive models that mimic the blood-brain barrier (BBB) hinders development effective drugs for neurodegenerative diseases. Animal behave differently from humans, are expensive and have ethical constraints. Organ-on-a-chip (OoC) platforms offer several advantages to resembling physiological pathological conditions in a versatile, reproducible, animal-free manner. In addition, OoC give us possibility incorporate sensors determine cell culture features such as trans-endothelial...
The local heat delivered by metallic nanoparticles selectively attached to their target can be used as a molecular surgery safely remove toxic and clogging aggregates. We apply this principle protein aggregates, in particular the amyloid beta (Aβ) involved Alzheimer's disease (AD), neurodegenerative where unnaturally folded Aβ proteins self-assemble deposit forming fibrils plaques. show possibility remotely redissolve these deposits interfere with growth, using dissipated gold (AuNP)...
Extracellular vesicles (EVs) have shown great potential for targeted therapy, as they a natural ability to pass through biological barriers and, depending on their origin, can preferentially accumulate at defined sites, including tumors. Analyzing the of EVs target specific cells remains challenging, considering unspecific binding lipophilic tracers other proteins, limitations fluorescence deep tissue imaging and effect external labeling strategies tropism. In this work, we determined...
Helicobacter pylori (Hp) infects the stomach of 50% world's population. Importantly, chronic infection by this bacterium correlates with appearance several extra-gastric pathologies, including neurodegenerative diseases. In such conditions, brain astrocytes become reactive and neurotoxic. However, it is still unclear whether highly prevalent or nanosized outer membrane vesicles (OMVs) they produce, can reach brain, thus affecting neurons/astrocytes. Here, we evaluated effects Hp OMVs on...
Niclosamide (NICLO) is a recognized antiparasitic drug being repositioned for Helicobacter pylori. The present work aimed to formulate NICLO nanocrystals (NICLO-NCRs) produce higher dissolution rate of the active ingredient and incorporate these nanosystems into floating solid dosage form release them stomach slowly. For this purpose, NICLO-NCRs were produced by wet-milling included in Gelucire l3D printed tablet semi-solid extrusion, applying Melting solidification printing process...
Breaking into cells: A new family of peptides based on the amphipathic proline-rich sequence (VXLPPP)n, where n=1–3 and X=His, Lys, or Arg, has been designed (see picture). Their nonviral origin, absence toxicity at high concentrations, solubility in water make this series very promising for use drug delivery.
Summary Lolium multiflorum (Italian ryegrass) seeds suspected of being resistant to glyphosate were collected from fruit orchards at two locations, San Bernardo (SB) and Olivar (OL), Chile, that had been treated an average three times per year with the isopropylamine salt during previous 8–10 years. Laboratory experiments conducted for each orchard population a susceptible population, commercial cultivar called Tama (TM), using Petri dishes containing filter paper saturated 5 mL solution...
One of the hallmarks Alzheimer's disease is self-aggregation amyloid beta peptide (Abeta) in extracellular fibrils. Among different forms Abeta, 42-residue fragment (Abeta1-42) readily self-associates and nucleation centers from where fibrils can quickly grow. The strong tendency Abeta1-42 to aggregate one reasons for scarcity data on its fibril formation process. We have used atomic force microscopy (AFM) visualize liquid environment fibrillogenesis synthetic hydrophilic hydrophobic...
In a previous work, we studied the interaction of β-amyloid fibrils (Aβ) with gold nanoparticles (AuNP) conjugated peptide CLPFFD-NH2. Here, effect changing residue sequence CLPFFD-NH2 on efficiency conjugation to AuNP, stability conjugates, and affinity conjugates Aβ fibrils. We AuNP isomeric peptides (CDLPFF-NH2 CLPDFF-NH2) characterized resulting different techniques including UV−Vis, TEM, EELS, XPS, analysis amino acids, agarose gel electrophoresis, CD. addition, determined proportion...
Background & Aims: Gold nanoparticles (GNPs) have promising applications for drug delivery as well the diagnosis and treatment of several pathologies, such those related to CNS. However, GNPs are retained in a number organs, liver spleen. Owing their negative charge and/or processes opsonization, by reticuloendothelial system, thereby decreasing brain. It is therefore crucial modify nanoparticle surface order increase its lipophilicity reduce charge, thus achieving enhanced Results: In this...
Gold nanoparticles (AuNPs) have been extensively used in biological applications because of their biocompatibility, size, and ease characterization, as well an extensive knowledge surface chemistry. These features make AuNPs readily exploitable for biomedical applications, including drug delivery novel diagnostic therapeutic approaches. In a previous work, we studied ex vivo distribution the conjugate C(AuNP)-LPFFD its potential uses treatment Alzheimer's disease. this study, covalently...