A5061653559- Caveolin-1 and cellular processes
- Bone Tissue Engineering Materials
- Signaling Pathways in Disease
- Cell Adhesion Molecules Research
- Cellular transport and secretion
- Cellular Mechanics and Interactions
- Lipid metabolism and biosynthesis
- Electrospun Nanofibers in Biomedical Applications
- Circular RNAs in diseases
- Metabolism, Diabetes, and Cancer
- Natural Antidiabetic Agents Studies
- PARP inhibition in cancer therapy
- Graphene and Nanomaterials Applications
- Collagen: Extraction and Characterization
- Additive Manufacturing and 3D Printing Technologies
- Advanced Glycation End Products research
- Cell death mechanisms and regulation
- Nutrition and Health in Aging
- Bone health and treatments
- Hippo pathway signaling and YAP/TAZ
- 3D Printing in Biomedical Research
- Clinical Nutrition and Gastroenterology
- Electrolyte and hormonal disorders
- Erythrocyte Function and Pathophysiology
- Endoplasmic Reticulum Stress and Disease
Viña del Mar University
2025
University of Chile
2012-2020
Federico Santa María Technical University
2017-2020
Universidad Bernardo O'Higgins
2016-2018
Advanced Center for Chronic Diseases
2014-2017
Instituto de Neurociencia Biomédica
2016
Pontificia Universidad Católica de Chile
2009
Migration and invasion are essential steps associated with tumor cell metastasis increasing evidence points towards endosome trafficking being in this process. Indeed, the small GTPase Rab5, a critical regulator of early dynamics, promotes migration vitro vivo. Precisely how Rab5 participates these events remains to be determined. Considering that focal adhesions represent structures crucial migration, we specifically asked whether activation promoted adhesion disassembly thereby facilitated...
Rab5 is a small GTPase that regulates early endosome trafficking and other cellular processes, including cell adhesion migration. Specifically, promotes Rac1 activation cancer migration, but little known about upstream regulators of Rab5. We have previously shown the scaffolding protein Caveolin-1 (CAV1) migration cells. Here, we hypothesized CAV1 stimulates activation, leading to increased activity Expression in B16-F10 mouse melanoma HT-29(US) human colon adenocarcinoma cells Rab5-GTP...
Caveolin-1 is known to promote cell migration, and increased caveolin-1 expression associated with tumor progression metastasis. In fibroblasts, polarization phosphorylation of tyrosine-14 are essential migration. However, the role in migration metastatic cells remains poorly defined. Here, participation was evaluated by shRNA targeting endogenous MDA-MB-231 human breast cancer ectopic B16-F10 mouse melanoma cells. Depletion reduced, while promoted focal adhesion turnover a sequence events...
Caveolin-1 (CAV1) is a scaffolding protein that plays dual role in cancer. In advanced stages of this disease, CAV1 expression tumor cells associated with enhanced metastatic potential, while, at earlier stages, functions as suppressor. We recently implicated phosphorylation on tyrosine 14 (Y14) CAV1-enhanced cell migration. However, the contribution modification to cancer remained unexplored. Here, we used vitro [2D and transendothelial migration (TEM), invasion] vivo (metastasis) assays,...
<title>Abstract</title> Background: Tumor-derived extracellular vesicles offer a minimally invasive approach to evaluate tumor progression and metastasis. However, detecting biomarkers, such as in body fluids during the early stages of disease, remains significant challenge. Conventional methods like ultracentrifugation-based isolation or Western blot protein quantification are time-consuming, require large sample volumes, low yield sensitivity. Therefore, development new biosensors for...
Caveolin-1 (CAV1), is a broadly expressed, membrane-associated scaffolding protein that acts both, as tumor suppressor and promoter of metastasis, depending on the type cancer stage. CAV1 downregulated in human tumors, cell lines oncogene-transformed cells. The activity generally associated with its presence at plasma membrane, where it participates, together cavins, formation caveolae also has been suggested to interact inhibit wide variety proteins through interactions mediated by domain....
Type 2 diabetes (T2D) can go undiagnosed for years, leading to a stage where produces complications such as delayed skin wound healing. Animal models have been developed in the last decades study pathological progression this disease. Streptozotocin (STZ), that has selective pharmacological toxicity toward pancreatic β cells, addition high fat diet widely used induce however no evidence shown its effects on integrity. Eighteen C57BL/6J male mice, were divided 3 groups; first was fed with...
Expression of the scaffolding protein Caveolin-1 (CAV1) enhances migration and invasion metastatic cancer cells. Yet, CAV1 also functions as a tumor suppressor in early stages cancer, where expression is suppressed by epigenetic mechanisms. Thus, we sought to identify stimuli/mechanisms that revert silencing cells evaluate how this affects their potential. We reasoned restricted tissue availability anti-neoplastic drugs during chemotherapy might expose sub-therapeutic concentrations, which...
Etoposide is widely used in the treatment of patients with testicular cancer. The mechanism underlying apoptosis induction cancer cells has been studied different cell types, but it not known whether same factors participate viable germ undergoing programmed death. Since primarily affects young males, we pubertal rats (21 days old) as a model to determine apoptotic parameters after etoposide healthy testes. We found that one intratesticular injection (1.2 µg/testis) induced significant...