A5054702560- Neuroscience and Neuropharmacology Research
- Neurogenesis and neuroplasticity mechanisms
- Memory and Neural Mechanisms
- Neuroscience of respiration and sleep
- Neuroinflammation and Neurodegeneration Mechanisms
- Tryptophan and brain disorders
- Stress Responses and Cortisol
- Neuroendocrine regulation and behavior
- Amyotrophic Lateral Sclerosis Research
- Epigenetics and DNA Methylation
- Neural dynamics and brain function
- Alzheimer's disease research and treatments
- Adipose Tissue and Metabolism
- Genetics and Neurodevelopmental Disorders
- Neonatal Respiratory Health Research
- Heart Rate Variability and Autonomic Control
- Diet and metabolism studies
- Nicotinic Acetylcholine Receptors Study
- Adenosine and Purinergic Signaling
- Multiple Sclerosis Research Studies
- Prenatal Substance Exposure Effects
- Infant Development and Preterm Care
- Infant Health and Development
- Nuclear Receptors and Signaling
- Neonatal and fetal brain pathology
Universidad de Santiago de Chile
2001-2024
Universidad Andrés Bello
2015-2020
Universidad Autónoma de Chile
2019
Universidad de Los Andes, Chile
2013-2017
Karolinska Institutet
2015
University of Chile
2015
Universidad de Los Andes
2007-2013
The Dlg4 gene encodes for post-synaptic density protein 95 (PSD95), a major synaptic that clusters glutamate receptors and is critical plasticity. PSD95 levels are diminished in ageing neurodegenerative disorders, including Alzheimer's disease Huntington's disease. epigenetic mechanisms (dys)regulate transcription of Dlg4/PSD95, or other plasticity genes, largely unknown, limiting the development targeted epigenome therapy. We analysed Dlg4/PSD95 landscape hippocampal tissue designed...
Aging compromises brain function leading to cognitive decline. A cyclic ketogenic diet (KD) improves memory in aged mice after long-term administration; however, short-term effects later life and the molecular mechanisms that govern such changes remain unclear. Here, we explore impact of a KD treatment starting at elderly stage on mice. Behavioral testing potentiation (LTP) recordings reveal working hippocampal LTP. Furthermore, synaptosome proteome fed evidence predominantly presynaptic...
Amyotrophic lateral sclerosis (ALS) is a fatal neurodegenerative disease in which pathogenesis and death of motor neurons are triggered by non-cell-autonomous mechanisms. We showed earlier that exposing primary rat spinal cord cultures to conditioned media derived from mouse astrocyte (ACM) express human SOD1(G93A) (ACM-hSOD1(G93A)) quickly enhances Nav channel-mediated excitability calcium influx, generates intracellular reactive oxygen species (ROS), leads motoneurons within days. Here we...
Objective To assess whether autoantibodies against ribosomal P (anti‐P), which are possibly pathogenic in neuropsychiatric systemic lupus erythematosus (NPSLE), alter glutamatergic synaptic transmission and to what extent the cross‐reacting neuronal surface antigen (NSPA) is involved. Methods We analyzed long‐term potentiation (LTP) mediated by AMPA receptor (AMPAR) N ‐methyl‐ d ‐aspartate (NMDAR) field excitatory postsynaptic potential (EPSP) at CA3–CA1 synapse. AMPAR activation patch‐clamp...
Clinically depressed individuals respond to different types of antidepressants, suggesting that neurobiological mechanisms may be responsible for their depression. However, animal models characterize this are not yet available. We induced depressive-like behaviors in rats using 2 chronic stress models: restraint small cages or immobilization adaptable plastic cones. Both increased anxiety responses evaluated by novelty-suppressed feeding and the elevated plus-maze; learned helplessness tail...
ORIGINAL RESEARCH article Front. Cell. Neurosci., 09 May 2013Sec. Cellular Neurophysiology https://doi.org/10.3389/fncel.2013.00066
Reelin, an extracellular glycoprotein secreted in embryonic and adult brain, participates neuronal migration plasticity. Extensive evidence shows that reelin via activation of the ApoER2 VLDLR receptors promotes dendrite spine formation during early development. Further suggests signaling is needed to maintain a stable architecture mature neurons, but, direct lacking. During activity‐dependent maturation circuitry, synaptic protein PSD95 inserted into postsynaptic membrane induce structural...
Dendrite arbor growth, or dendritogenesis, is choreographed by a diverse set of cues, including the NMDA receptor (NMDAR) subunits NR2A and NR2B. While NR1NR2B receptors are predominantly expressed in immature neurons promote plasticity, NR1NR2A mainly mature induce circuit stability. How different regulate these processes unclear, but this likely related to presence their distinct C-terminal sequences that couple signaling proteins. Calcium-calmodulin-dependent protein kinase II (CaMKII) an...
Hexanucleotide repeat expansions of the G4C2 motif in a non-coding region C9ORF72 gene are most common genetic cause amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD). Tissues from C9ALS/FTD patients mouse models ALS show RNA foci, dipeptide-repeat proteins, notably, widespread alterations transcriptome. Epigenetic processes regulate expression without changing DNA sequences therefore could account for altered transcriptome profiles C9ALS/FTD; here, we explore whether...
Astrocytes play a critical role in the maintenance of healthy central nervous system and astrocyte dysfunction has been implicated various neurodegenerative disorders, including amyotrophic lateral sclerosis (ALS) frontotemporal dementia (FTD). There is compelling evidence that mouse human ALS ALS/FTD astrocytes can reduce number wild-type motoneurons (MNs) co-cultures or after treatment with conditioned media (ACM), independently their genotype. A growing studies have shown soluble toxic...
Multiple sclerosis (MS) is a chronic and degenerative disease that impacts central nervous system (CNS) function. One of the major characteristics presence regions lacking myelin an oxidative inflammatory environment. TGF-β1 Nrf2 proteins play fundamental role in different oxidative/inflammatory processes linked to neurodegenerative diseases such as MS. The evidence from experimental settings has demonstrated TGF-β1-Nrf2 signaling crosstalk under pathological conditions. However, this...
Acylpeptide hydrolase (APEH) is a serine that displays two catalytic activities, acting both as an exopeptidase toward short N-acylated peptides and endopeptidase oxidized or proteins. It has been demonstrated this enzyme can degrade monomers, dimers, trimers of the A1–40 peptide in conditioned media neuroblastoma cells. In previous report, we showed specific inhibition by organophosphate molecule dichlorvos (DDVP) triggers enhancement long-term potentiation rat hippocampal slices. study,...
Fluoxetine is a selective serotonin reuptake inhibitor (SSRI) used to treat mood and anxiety disorders. Chronic treatment with this antidepressant drug thought favor functional recovery by promoting structural molecular changes in several forebrain areas. At the synaptic level, chronic fluoxetine induces an increased size density of dendritic spines ratio GluN2A over GluN2B N-methyl-D-aspartate (NMDA) receptor subunits. The "maturation"-promoting observed after should also induce remodeling...
N-methyl-D-aspartate receptors (NMDARs) that contain the NR2A and NR2B subunits play a critical role in neuronal plasticity dendritogenesis. Gain-and-loss-of function studies indicate NR2B, but not NR2A, promotes dendritic branching. Accumulating evidence indicates stimulation of NMDARs activates NADPH oxidase (NOX2), thereby generating superoxide. However, molecular underpinnings this process are understood. RasGRF1, guanine nucleotide exchange factor, is key for several forms interacts...
Since its introduction two decades ago, the isolated brain stem-spinal cord preparation of neonatal rodents has been preferred method used to reveal mystery underlying genesis respiratory rhythm. Little research using this in vitro approach focused on study central chemosensitivity. Some unexpected findings obtained with have added new questions that challenge our previous theoretic framework. these are addressed here.
The continuous appearance of SARS-CoV-2 variants with both increased transmission and immune evading potential, in addition to the reluctance some populations be vaccinated, supports search for alternative treatments protect against viral infection consequences. Bromhexine is a well-known, over-the counter, expectorant which have garnered interest potential prevention treatment COVID-19. Here, we report results placebo-controlled randomized clinical trial efficacy oral bromhexine was tested...
A well-recognized molecular entity involved in pain-related neuroplasticity is the N-methyl-D-aspartate receptor (NMDAR), which crucial for developing chronic pain. Likewise, pannexin 1 (Panx1) channel has been described as necessary initiating and maintaining neuropathic pain, driving nociceptive signals dependent on spinal NMDAR through several possible mechanisms. Through behavioral, pharmacological, approaches, our study male rats revealed key findings: (1) neurons located cord laminae I...