A5055022305- Cellular transport and secretion
- Cell Adhesion Molecules Research
- Angiogenesis and VEGF in Cancer
- Receptor Mechanisms and Signaling
- Sperm and Testicular Function
- Galectins and Cancer Biology
- Proteoglycans and glycosaminoglycans research
- RNA Interference and Gene Delivery
- Monoclonal and Polyclonal Antibodies Research
- Mitochondrial Function and Pathology
- Autophagy in Disease and Therapy
- Caveolin-1 and cellular processes
- Ubiquitin and proteasome pathways
- Alzheimer's disease research and treatments
- Glycosylation and Glycoproteins Research
- Microbial Metabolic Engineering and Bioproduction
- Protease and Inhibitor Mechanisms
- Genetic and Kidney Cyst Diseases
- Endoplasmic Reticulum Stress and Disease
- Viral Infectious Diseases and Gene Expression in Insects
- Neuroinflammation and Neurodegeneration Mechanisms
- ATP Synthase and ATPases Research
- Epigenetics and DNA Methylation
- Parasitic infections in humans and animals
- Hedgehog Signaling Pathway Studies
San Sebastián University
2017-2023
Fundación Ciencia and Vida
2022
University of Concepción
2020
Pontificia Universidad Católica de Chile
2003-2018
University of Mons
2012-2018
State University of Norte Fluminense
2004-2012
Pontificia Universidad Católica de Valparaíso
2009
University of Chile
1988-2000
Accumulation of extracellular amyloid β peptide (Aβ), generated from precursor protein (APP) processing by β- and γ-secretases, is toxic to neurons central the pathogenesis Alzheimer disease. Production Aβ APP greatly affected subcellular localization trafficking APP. Here we have identified a novel intracellular adaptor protein, sorting nexin 17 (SNX17), that binds specifically cytoplasmic domain via YXNPXY motif has been shown previously bind several cell surface adaptors, including Fe65...
To define whether anti-ribosomal P (anti-P) autoantibodies from patients with neuropsychiatric systemic lupus erythematosus (NPSLE) impair the function of hippocampal neurons that express neuronal surface antigen (NSPA) when accessing brain via circulating blood.We used anti-P antibodies NPSLE and rabbit-generated anti-NSPA antibodies. Primary mice were analyzed to determine antibody cell binding (double immunofluorescence), intracellular calcium variations (Fura 2 AM), apoptosis (caspase 3...
The effect of temperature on the kinetic parameters involved in main reactions anaerobic digestion process was studied. Batch tests with starch, glucose and acetic acid as substrates for hydrolysis, acidogenesis methanogenesis, respectively, were performed a range between 15 45°C. First order kinetics assumed to determine hydrolysis rate constant, while Monod Haldane considered respectively. results obtained showed that is strongly influenced by temperature, exerting highest effect. Cardinal...
Objective To assess whether autoantibodies against ribosomal P (anti‐P), which are possibly pathogenic in neuropsychiatric systemic lupus erythematosus (NPSLE), alter glutamatergic synaptic transmission and to what extent the cross‐reacting neuronal surface antigen (NSPA) is involved. Methods We analyzed long‐term potentiation (LTP) mediated by AMPA receptor (AMPAR) N ‐methyl‐ d ‐aspartate (NMDAR) field excitatory postsynaptic potential (EPSP) at CA3–CA1 synapse. AMPAR activation patch‐clamp...
The p75 neurotrophin receptor (p75) is a multifaceted signalling that regulates neuronal physiology, including neurite outgrowth and survival death decisions. A key cellular aspect regulating the intracellular trafficking of their receptors; however, post-endocytic poorly defined. We used sympathetic neurons PC12 cells to study internalization mechanism p75. found depended on clathrin adaptor protein AP2 dynamin. More surprising, evaded lysosomal route at level early endosome, accumulating...
The term vasculogenic mimicry (VM) refers to the capacity of certain cancer cells form fluid-conducting structures within a tumor in an endothelial cell (EC)-free manner. Ever since its first report by Maniotis 1999, existence VM has been extremely contentious issue. overwhelming consensus literature suggests that is frequently observed highly aggressive tumors and correlates lower patient survival. While presence vivo animal are claimed upon strong positive staining for glycoproteins...
Megalin and the low‐density lipoprotein (LDL) receptor‐related protein (LRP) are two large members of LDL receptor family that bind endocytose multiple ligands. The molecular cellular determinants dictate sorting behavior these receptors in polarized epithelial cells largely unknown. is found apically distributed, whereas limited information on LRP indicates its polarity. We show here Madin‐Darby canine kidney cells, both endogenous a minireceptor containing fourth ligand‐binding,...
Low-density lipoprotein receptor-related protein 1 (LRP1) is an endocytic recycling receptor with two cytoplasmic tyrosine-based basolateral sorting signals. Here we show that during biosynthetic trafficking LRP1 uses AP1B adaptor complex to move from a post-TGN endosome (RE) the membrane. Then it recycles basolaterally (BSE) involving recognition by nexin 17 (SNX17). In pathway, Y(29) but not N(26) proximal NPXY directs TGN. A N(26)A mutant revealed this motif recognized SNX17 required for...
Abstract Vasculogenic mimicry (VM) describes a process by which cancer cells establish an alternative perfusion pathway in endothelial cell-free manner. Despite its strong correlation with reduced patient survival, controversy still surrounds the existence of vitro model VM. Furthermore, many studies that claim to demonstrate VM fail provide solid evidence true hollow channels, raising concerns as whether actual is actually being examined. Herein, we standardized assay recreates formation...
The apolipoprotein E receptor 2 (apoER2) is a member of the low‐density lipoprotein family which binds ligands such as reelin, and J/clusterin has been shown to play roles in neuronal migration during development male fertility. function apoER2 mainly depends on cellular signaling triggered by ligand binding. Although internalized, mechanism functional significance its endocytic trafficking remain unclear. Apolipoprotein partitions into lipid rafts interacts with caveolin‐1, feature that...
Epithelial cells can acquire invasive and tumorigenic capabilities through epithelial-mesenchymal-transition (EMT). The glycan-binding protein galectin-8 (Gal-8) activates selective β1-integrins involved in EMT is overexpressed by certain carcinomas. Here we show that Gal-8 overexpression or exogenous addition promotes proliferation, migration, invasion nontumoral Madin-Darby canine kidney (MDCK) cells, involving focal-adhesion kinase (FAK)-mediated transactivation of the epidermal growth...
Endocytosis modulates EGFR function by compartmentalizing and attenuating or enhancing its ligand-induced signaling. Here we show that it can also control the cell surface versus intracellular distribution of empty/inactive EGFR. Our previous observation PKA inhibitors induce internalization prompted us to test phosphatidic acid (PA) generated phospholipase D (PLD) as an endogenous down-regulator activity, which activates rolipram-sensitive type 4 phosphodiesterases (PDE4) degrade cAMP. We...
Epidermal growth factor receptor ( EGFR ) exaggerated (oncogenic) function is currently targeted in cancer treatment with drugs that block ligand binding or tyrosine kinase activity. Because endocytic trafficking a crucial regulator of function, its pharmacological perturbation might provide new anti‐tumoral strategy. Inhibition phosphatidic acid PA phosphohydrolase PAP activity has been shown to trigger signaling towards type 4 phosphodiesterase PDE 4) activation and protein A inhibition,...
Ligand-independent epidermal growth factor receptor (EGFR) endocytosis is inducible by a variety of stress conditions converging upon p38 kinase. A less known pathway involves phosphatidic acid (PA) signaling toward the activation type 4 phosphodiesterases (PDE4) that decrease cAMP levels and protein kinase (PKA) activity. This PA/PDE4/PKA triggered with propranolol used to inhibit PA hydrolysis induces clathrin-dependent clathrin-independent endocytosis, followed reversible accumulation...
The neurulation process is regulated by a large amount of genetic and environmental factors that determine the establishment, folding, fusion neural plate to form tube, which develops into main structure central nervous system. A recently described factor involved in this glutamate. Through NMDA ionotropic receptor, glutamate modifies intracellular Ca2+ dynamics allowing oriented cell migration proliferation, essentials processes neurulation. Glutamate synthesis depends on mitochondrial...
Cancer therapy may be improved by the simultaneous interference of two or more oncogenic pathways contributing to tumor progression and aggressiveness, such as EGFR p53. Tumor cells expressing gain-of-function (GOF) mutants p53 (mutp53) are usually resistant inhibitors display invasive migration AKT-mediated survival associated with enhanced recycling. D-Propranolol (D-Prop), non-beta blocker enantiomer propranolol, was previously shown induce internalization through a PKA inhibitory pathway...
Decorin is a small leucine-rich proteoglycan that modulates the activity of transforming growth factor type β and other factors thereby influences processes proliferation differentiation in wide array physiological pathological reactions. Hence, understanding regulatory mechanisms decorin has broad implications. Here we report extracellular levels are controlled by receptor-mediated catabolism, involving low density lipoprotein receptor family member, receptor-related protein (LRP). We show...