A5063013427- Galectins and Cancer Biology
- Alzheimer's disease research and treatments
- Glycosylation and Glycoproteins Research
- Protein Tyrosine Phosphatases
- Mitochondrial Function and Pathology
- Cell Adhesion Molecules Research
- Neuroscience and Neuropharmacology Research
- Signaling Pathways in Disease
- Autophagy in Disease and Therapy
- Radiomics and Machine Learning in Medical Imaging
- Hepatocellular Carcinoma Treatment and Prognosis
- Analytical Chemistry and Sensors
- Advanced Radiotherapy Techniques
- Biochemical effects in animals
- Advanced MRI Techniques and Applications
- Pain Management and Opioid Use
- Pelvic and Acetabular Injuries
- Pancreatic function and diabetes
- Genetic Neurodegenerative Diseases
- Adenosine and Purinergic Signaling
- Bioinformatics and Genomic Networks
- Cancer survivorship and care
- Spine and Intervertebral Disc Pathology
- T-cell and Retrovirus Studies
- Celiac Disease Research and Management
University of Virginia
2021-2024
Pontificia Universidad Católica de Chile
2006-2019
Hospital Universitario Araba
2019
Center for Climate and Resilience Research
2018
University of Chile
2017
Beatson West of Scotland Cancer Centre
2015
CEA Valduc
2015
Hospital de Cruces
2013
Millennium Institute for Integrative Biology
2009
Galectins have been implicated in T cell homeostasis playing complementary pro-apoptotic roles. Here we show that galectin-8 (Gal-8) is a potent agent Jurkat cells inducing complex phospholipase D/phosphatidic acid signaling pathway has not reported for any galectin before. Gal-8 increases phosphatidic signaling, which enhances the activity of both ERK1/2 and type 4 phosphodiesterases (PDE4), with subsequent decrease basal protein kinase A activity. Strikingly, rolipram inhibition PDE4...
The interruption of endoplasmic reticulum (ER)-mitochondrial Ca
Galectin-8 (Gal-8) is a member of glycan-binding protein family that regulates the immune system, among other functions, and target antibodies in autoimmune disorders. However, its role multiple sclerosis (MS), an inflammatory disease central nervous system (CNS), remains unknown. We study consequences Gal-8 silencing on lymphocyte subpopulations development experimental encephalitis (EAE), to then assess presence clinical meaning anti-Gal-8 MS patients. Lgals8/Lac-Z knock-in mice lacking...
Epithelial cells can acquire invasive and tumorigenic capabilities through epithelial-mesenchymal-transition (EMT). The glycan-binding protein galectin-8 (Gal-8) activates selective β1-integrins involved in EMT is overexpressed by certain carcinomas. Here we show that Gal-8 overexpression or exogenous addition promotes proliferation, migration, invasion nontumoral Madin-Darby canine kidney (MDCK) cells, involving focal-adhesion kinase (FAK)-mediated transactivation of the epidermal growth...
Neuronal nuclei are normally smoothly surfaced. In Alzheimer's disease (AD) and other tauopathies, though, they often develop invaginations. We investigated mechanisms functional consequences of neuronal nuclear invagination in tauopathies.
Reduced brain energy metabolism, mammalian target of rapamycin (mTOR) dysregulation, and extracellular amyloid beta (Aβ) oligomer (xcAβO) buildup are some well-known Alzheimer's disease (AD) features; how they promote neurodegeneration is poorly understood. We previously reported that xcAβOs inhibit nutrient-induced mitochondrial activity (NiMA) in cultured neurons. now report NiMA disruption vivo.
The role of autoantibodies in the pathogenesis systemic lupus erythematosus (SLE) has not been completely defined. From more than a hundred described SLE, relatively few have associated with clinical manifestations. glycan-binding proteins galectin family can modulate immune system. Anti-galectin thus could functional and/or pathogenic implications inflammatory processes and autoimmunity. We previously reported function-blocking against galectin-8 (Gal-8) SLE. Here we tested these series...
Altered mitochondrial DNA (mtDNA) occurs in neurodegenerative disorders like Alzheimer's disease (AD); how mtDNA synthesis is linked to neurodegeneration poorly understood. We previously discovered Nutrient-induced Mitochondrial Activity (NiMA), an inter-organelle signaling pathway where nutrient-stimulated lysosomal mTORC1 activity regulates replication neurons by a mechanism sensitive amyloid-β oligomers (AβOs), primary factor AD pathogenesis (Norambuena et al., 2018). Using...
Galectin-8 belongs to a family of mammalian lectins that recognize glycoconjugates present on diff erent cell surface components and modulate variety cellular processes.A role Gal-8 in the immune system has been proposed based its eff ects cells, including T B lymphocytes, as well presence anti-Gal-8 autoantibodies prototypic autoimmune disease systemic lupus erythematosus (SLE).We have previously described induces apoptosis activated cells interacting with certain β1 integrins this ect is...
Abstract Introduction Reduced brain energy metabolism, mTOR dysregulation, and extracellular amyloid-β oligomer (xcAβO) buildup characterize AD; how they collectively promote neurodegeneration is poorly understood. We previously reported that xcAβOs inhibit N utrient-induced M itochondrial A ctivity (NiMA) in cultured neurons. now report NiMA disruption vivo . Methods Brain metabolism oxygen consumption were recorded APP SAA/+ mice using two-photon fluorescence lifetime imaging...
Extracellular nucleotides transmit signals into the cells through P2 family of cell surface receptors. These receptors are amply expressed in human blood vessels and participate vascular tone control; however, their signaling mechanisms remain unknown. Here we show that smooth muscle isolated chorionic arteries, activation P2Y2 receptor (P2Y2R) induces not only its partition membrane rafts but also rapid internalization. Cholesterol depletion with methyl-β-cyclodextrin reduced association...
Antibodies against galectin-8 in patients with systemic lupus erythematosus
Alterations in NADH and NADPH metabolism are associated with aging, cancer, Alzheimer’s Disease. Using 2P-FLIM imaging of the mitochondrial NAD(P)H live human neurons PS19 mouse brains, we show that tau oligomers (TauO) upregulate de novo synthesis through NADK2. This process controls LRP1-mediated internalization TauO, setting a vicious cycle for further TauO internalization. Thus, NADK2-dependent key step toxicity.
The advances in the field of optics and laser technologies are helpful to visualize investigate metabolism live animals. Fluorescence lifetime imaging (FLIM) is a non-invasive optical technique measure fluorescence fluorophore towards its applications where intensity-based techniques do not provide sufficient accurate information discriminate auto-fluorescent species. In contrast conventional single photon excitation, two-photon excitation improves penetration depth with low scattering...
Purpose/Objective: Motion management is a major challenge in abdominal SBRT.We present our study of SBRT for primary or secondary liver tumors using intrafraction motion review (IMR) which allows simultaneous KV information and /MV delivery to synchronize the beam during gated RapidArc treatment.Materials Methods: Between May 2012 April 2014, 25 consecutive patients were treated with curative-intent SBRT.All technique Varian Novalis Truebeam STx linear accelerator.An isotropic margin 5mm...
s: XIVth Congress of the Pan-American League Associations for Rheumatology: CATEGORY: SLE: Pathogenesis
Abstract Altered mitochondrial DNA (mtDNA) occurs in neurodegenerative disorders like Alzheimer’s disease (AD); how mtDNA synthesis is linked to neurodegeneration poorly understood. We discovered N utrient-induced M itochondrial A ctivity (NiMA), an inter-organelle signaling pathway where nutrient-stimulated lysosomal mTORC1 activity regulates replication neurons by a mechanism sensitive amyloid-β oligomers (AβOs), primary factor AD pathogenesis. Using 5-ethynyl-2’-deoxyuridine (EdU)...