A5030825539- Estrogen and related hormone effects
- Gastric Cancer Management and Outcomes
- Breast Cancer Treatment Studies
- Cancer Cells and Metastasis
- Colorectal Cancer Treatments and Studies
- Angiogenesis and VEGF in Cancer
- Cancer Risks and Factors
- Gastrointestinal Tumor Research and Treatment
- Cancer-related molecular mechanisms research
- Global Cancer Incidence and Screening
- Cancer survivorship and care
- Endometrial and Cervical Cancer Treatments
- HER2/EGFR in Cancer Research
- Cancer, Lipids, and Metabolism
- Cancer, Hypoxia, and Metabolism
- RNA modifications and cancer
- Ovarian cancer diagnosis and treatment
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Cancer Immunotherapy and Biomarkers
- Metabolism, Diabetes, and Cancer
- Blood Coagulation and Thrombosis Mechanisms
- Cell Adhesion Molecules Research
- Helicobacter pylori-related gastroenterology studies
- Lung Cancer Treatments and Mutations
- Adrenal and Paraganglionic Tumors
Pontificia Universidad Católica de Chile
2014-2024
Brown University
2024
Universidad Mayor
2022-2023
Hospital General de Agudos Dr. TEODORO ALVAREZ
2021
Hôpital de la Croix-Rousse
2021
Chalmers University of Technology
2019
Federación Colombiana de Obstetricia y Ginecología
2019
Hôpital Edouard Herriot
2019
Universidad de Santiago de Chile
2016-2018
Universidad Andrés Bello
2014-2015
There is evidence that primary aldosteronism (PA) may be common in patients with essential hypertension (EH) when determinations of serum aldosterone (SA), plasma renin activity (PRA), and the SA/PRA ratio are used as screening. An inherited form hyperaldosteronism glucocorticoid-remediable (GRA) caused by an unequal crossing over between CYP11B1 CYP11B2 genes results a chimeric gene, which has synthase regulated ACTH. The aim this study was to evaluate prevalence PA GRA 305 EH 205...
High-grade serous ovarian cancer (HGSOC) is the most prevalent and aggressive histologic type of cancer. To date, there are no reliable biomarkers to effectively predict patient prognosis. Studies have demonstrated inflammation tumor infiltrating lymphocytes (TILs) correlate with a bad good prognosis, respectively. Here, we sought evaluate systemic TILs as early prognostic markers survival.Neutrophil-to-lymphocyte ratio (NLR) serum Lactate Dehydrogenase (LDH) were used indicators...
Luminal breast cancers express estrogen (ER) and/or progesterone (PR) receptors and respond to hormone therapies. Basal-like “triple negative” lack steroid but are cytokeratin (CK) 5-positive require chemotherapy. Here we show that more than half of primary ER + PR contain an − CK5 “luminobasal” subpopulation exceeding 1% cells. Starting from luminal cell lines, generated lines with varying luminobasal ratios studied their molecular biological properties. In disease, cells expand in response...
Abstract Introduction Luminal, estrogen receptor-positive (ER + ) breast cancers can metastasize but lie dormant for years before recurrences prove lethal. Understanding the roles of (E) or progestin (P) in development luminal metastases arousal from dormancy is hindered by few preclinical models. We have developed such Methods Immunocompromised, ovariectomized (ovx’d) mice were intracardiac-injected with basal human cancer cells. Four lines tested: ER PR cytokeratin 5-negative (CK5 − E3 and...
Luminal, estrogen receptor-positive breast cancers represent more than 70% of cases. Despite initial good prognoses one third Luminal eventually recur locally or at distant sites and exhibit hormone resistance. Here we demonstrate that factors elaborated by malignant stromal cells can induce tumor proliferation promote angiogenesis independence. We recently isolated a mouse mammary gland cell line named BJ3Z increases in estrogen-free xenografted MCF-7 cancer cells. Normal Fibroblasts (NMFs)...
The term vasculogenic mimicry (VM) refers to the capacity of certain cancer cells form fluid-conducting structures within a tumor in an endothelial cell (EC)-free manner. Ever since its first report by Maniotis 1999, existence VM has been extremely contentious issue. overwhelming consensus literature suggests that is frequently observed highly aggressive tumors and correlates lower patient survival. While presence vivo animal are claimed upon strong positive staining for glycoproteins...
Abstract Vasculogenic mimicry (VM) describes a process by which cancer cells establish an alternative perfusion pathway in endothelial cell-free manner. Despite its strong correlation with reduced patient survival, controversy still surrounds the existence of vitro model VM. Furthermore, many studies that claim to demonstrate VM fail provide solid evidence true hollow channels, raising concerns as whether actual is actually being examined. Herein, we standardized assay recreates formation...
Breast cancer-related lymphedema (BCRL) is characterized by arm swelling, pain, and discomfort, reducing the quality of life (QoL) affected individuals. BRCL caused via blockage or disruption lymphatic vessels following cancer treatments, leading to an accumulation fluid in arm. While current BCRL rehabilitation treatments seek reduce our study aimed examine impact both magnitude (ΔVolume) disability on three dimensions QoL: social, physical, psychological. Using Disabilities Arm, Shoulder,...
"Reactive" or activated stroma characterizes many malignancies including breast cancers. Recently, we isolated a reactive mouse mammary gland stromal cell line called BJ3Z. These cells express alpha-smooth muscle actin and cell-derived factor 1 (SDF-1) are tumorigenic when injected into mice. Here show that, in vivo, BJ3Z influence the angiogenesis proliferation of xenografted estrogen receptor (ER)-positive MCF-7 human cancer solid tumors. The growth-promoting effects equivalent to those...
Porphyromonas gingivalis ( P. ) is a gram-negative oral pathogen associated with chronic periodontitis. Previous studies have linked poor health and periodontitis cancer. Severe cases of periodontal disease can result in advanced periodontitis, leading to tissue degradation, tooth loss, may also correlate higher gastric cancer (GC) risk. In fact, loss an elevated risk However, the clinical evidence for this association remains inconclusive. Periodontitis characterized by inflammation...
Progesterone in hormonal preparations increases the incidence of breast cancer. Tissue factor (TF), initiator extrinsic coagulation pathway, is associated with metastasis a wide variety cancers. We demonstrate herein that TF mRNA and protein are up-regulated by progesterone cancer cell line ZR-75. Epidermal growth factor, also increased risk, did not regulate TF. The increase both rapid transient; increasing after 6 h, reaching maximum at 24 before decreasing to basal levels 72 h. Sucrose...
Abstract The lymphatic system is a common avenue for the spread of breast cancer cells and dissemination through it occurs at least as frequently hematogenous metastasis. Approximately 75% primary cancers are estrogen receptor (ER) positive majority these maintain expression lymph node (LN) metastases. However, unknown if ER function equivalent in growing LNs. We have developed model to assess responsiveness ER+ tumors LN Fluorescent MCF-7 were grown ovariectomized nude mice supplemented...
The development of in vitro three dimensional (3D) cell culture matrices offers physiologically relevant alternatives to traditional on plastic surfaces. However methods analyze subpopulations therein are poor. Here we present a simple and inexpensive method mixed colonies using standard immunohistochemical (IHC) techniques. Briefly, MatrigelTM blocks sandwiched between two layers HistoGelTM, hardened by rapid cooling then processed for routine fixation, paraffin embedding IHC. We...
Breast cancer incidence increases in women receiving combined estrogen and progesterone therapy. tumors show increased expression of the glucose transporter GLUT1. We determined effect these hormones on GLUT1–4 deoxyglucose transport ZR-75-1 breast cells. Immunoblotting, immunocytochemistry, flow cytometry, RT-PCR showed that GLUT1 is up-regulated by and, to a greater degree, GLUT2 unaffected hormonal treatment. GLUT3 protein RNA therapy, GLUT4 all treatments. Deoxyglucose studies revealed...
Gastric cancer (GC) is a heterogeneous disease. This heterogeneity applies not only to morphological and phenotypic features but also geographical variations in incidence mortality rates. As Chile has one of the highest rates within South America, we sought define molecular profile Chilean GCs (ClinicalTrials.gov identifier: NCT03158571/(FORCE1)). Solid tumor samples clinical data were obtained from 224 patients, with subsets analyzed by tissue microarray (TMA; n = 90) next generation...
Estrogen replacement therapy and other unopposed estrogen treatments increase the incidence of endometrial abnormalities, including cancer. However, this effect is counteracted by co-administration progesterone. In endometrium, glucose transporter (GLUT) expression transport are known to fluctuate throughout menstrual cycle. Here, we determined progesterone on GLUT1-4 deoxyglucose in Ishikawa cancer cells. Cells were incubated with estrogen, or combined for 24 h was determined. We show that...
Abstract Increased glucose uptake as a principal energy source is requirement for the continued survival of tumour cells. Facilitative transporter‐1 (GLUT1) and ‐3 (GLUT3) have been previously shown to be present regulated in breast cancer cells are associated with poor patient prognosis. In cells, cAMP secondary messenger pathway known potentiate described transporter activators regulate cell fate. However, no regulation transporters by has examined. Herein, we determined well‐characterized...
Hypercoagulable state is linked to cancer progression; however, the precise role of coagulation cascade poorly described. Herein, we examined contribution a hypercoagulative through administration intravenous Coagulation Factor Xa (FXa), on growth solid human tumors and experimental metastasis B16F10 melanoma in mouse models. FXa increased tumor volume lung, liver, kidney lymph node tail-vein injected cells. Concentrating model, upon coadministration anticoagulant Dalteparin, lung was...