A5000051211- Platelet Disorders and Treatments
- Antiplatelet Therapy and Cardiovascular Diseases
- Blood Coagulation and Thrombosis Mechanisms
- Forensic Toxicology and Drug Analysis
- Venous Thromboembolism Diagnosis and Management
- Alcohol Consumption and Health Effects
- Connexins and lens biology
- Blood properties and coagulation
- Adipokines, Inflammation, and Metabolic Diseases
- Heparin-Induced Thrombocytopenia and Thrombosis
- Lipid metabolism and disorders
- Heat shock proteins research
- Diabetes, Cardiovascular Risks, and Lipoproteins
- Receptor Mechanisms and Signaling
- Neonatal Respiratory Health Research
- Tryptophan and brain disorders
- Complement system in diseases
- Cardiac Health and Mental Health
- Healthcare Technology and Patient Monitoring
- Cardiovascular Health and Disease Prevention
- Cardiac Arrhythmias and Treatments
- Sulfur Compounds in Biology
- Sepsis Diagnosis and Treatment
- Pharmacological Receptor Mechanisms and Effects
- Educational Research and Science Teaching
Pontificia Universidad Católica de Chile
2015-2024
Clínica Alemana
2011
University of Chile
1999-2011
Universidad del Desarrollo
2011
Hospital Clínico de la Universidad de Chile
2010
National Cancer Institute
2007
Fundación Chile
1999
Albert Einstein College of Medicine
1990-1993
The risk for thrombosis is significantly increased in systemic lupus erythematosus (SLE), affecting both venous and arterial vessels. Activated platelets are known to participate thrombus formation growth. A general feature of activated cells the shedding microparticles (MP) which support coagulation by exposure negatively charged phospholipids possibly tissue factor (TF). In this work we characterized circulating MP patients with SLE their relationship a procoagulant state. Thirty (aged...
Myosin is a ubiquitous eukaryotic contractile protein that generates the force responsible for such diverse cellular movements as muscle contraction and cytokinesis. Although there have been numerous studies of sarcomeric myosin heavy chain (MHC) genes, no molecular clones reported encode mammalian nonmuscle MHC. This study presents genetic characterization human MHC expressed in fibroblasts, endothelial cells, macrophages. Human amino acids are weakly homologous (33%) to but approximately...
Abstract Background Several studies have validated capillary refill time (CRT) as a marker of tissue hypoperfusion, and recent guidelines recommend CRT monitoring during septic shock resuscitation. Therefore, it is relevant to further explore its kinetics response short-term hemodynamic interventions with fluids or vasopressors. A couple previous explored the impact fluid bolus on CRT, but little known about norepinephrine when aiming at higher mean arterial pressure (MAP) target in shock....
Immunofluorescence and immunogold electron microscopy were employed to examine the assembly of connexins (Cx) 33, 37, 43 into testis cell gap junctions in mature postnatal rats. C×37 was localized by immunofluorescence endothelia blood vessels both immature testes not further characterized. Only C×43 assembled Leydig junctions, but also co-assembled with Cx33 some Sertoli-Sertoli junction plaques within near Sertoli occluding on adluminal surfaces. Assembly appeared be regulated according...
Because hepatocytes communicate via gap junctions, it has been proposed that Ca 2+ waves propagate through this pathway and in the process activate -dependent cellular responses. We tested hypothesis by measuring vasopressin-induced glycogenolysis short-term cultures of rat hepatocytes. A 15-min vasopressin (10 −8 M) stimulation induced a reduction glycogen content reached maximum 1–3 h later. Gap junction blockers, octanol or 18α-glycyrrhetinic acid, reduced effect 70%. The glycogenolytic...
Objective: To evaluate the impact of syringe size on start-up delay and time to reach 50% 90% target flow rates, using two commercially available infusion pumps at rates ≤1 mL/hr. Design: Two syringes (Terumo) different (10-mL 50-mL), (Pump A, Terumo Terufusion Infusion Pump TE-331; B, Braun Perfusor Compact S) were studied. Effective fluid delivery was measured 0.4 mL/hr, 0.8 1.0 mL/hr for initial 60 mins, gravimetric method. Instant calculated as volume difference every 1-min interval per...
Syrian Golden hamsters develop severe emphysema after a single intratracheal dose of elastase, whereas Sprague-Dawley rats exhibit mild with the same per kilogram body weight. We hypothesized that development is prevented in by high serum level alpha1-antitrypsin reported rats, compared hamsters, which provides for lung elastase inhibitory capacity (EIC). To explore this possibility, we challenged antiprotease system treating them three similar weekly doses elastase. Four months treatment,...
Background: Metabolic syndrome, a chronic condition associated with higher risk of cardiovascular diseases, is increasingly prevalent in young adults. Dyslipidemia, proinflammatory cytokines, endothelial dysfunction signs, and RhoA/Rho-kinase (ROCK) activation are considered factors diseases. The occurrence these patients metabolic syndrome but without type 2 diabetes or hypertension has not been fully studied. objective this study was to evaluate subjects enlarged waist circumference...
Cocaine abuse increases the risk of cardiac and cerebrovascular events, such as myocardial infarction ischemic stroke. The underlying mechanisms leading to these complications are not fully understood although intravascular thrombus formation has been observed. aim this study was investigate existence platelet activation effect short-term abstinence in chronic cocaine consumers. We studied 23 dependent individuals (aged 20-54 years) who met DSM-IV criteria for dependence 20 controls. Samples...
Hypercoagulable state is linked to cancer progression; however, the precise role of coagulation cascade poorly described. Herein, we examined contribution a hypercoagulative through administration intravenous Coagulation Factor Xa (FXa), on growth solid human tumors and experimental metastasis B16F10 melanoma in mouse models. FXa increased tumor volume lung, liver, kidney lymph node tail-vein injected cells. Concentrating model, upon coadministration anticoagulant Dalteparin, lung was...
Platelets have a major role in clotting activation and contribute to the innate immune response during systemic infections. Human platelets contain tissue factor (TF) express functional Toll-like receptor 4 (TLR4). However, of TLR4 triggering procoagulant properties platelets, upon challenge with bacteria, is yet unknown. Our hypothesis that E. coli O111-TLR4 interaction activates elicits their activity. We demonstrated strain, but not ultrapure LPS, increased surface P-selectin expression,...
Knockout models have shown that the coagulation system has a role in vascular development and angiogenesis. Herein, we report for first time zymogen FX its active form (FXa) possess anti-angiogenic properties. Both recombinant FXa inhibit angiogenesis vitro using endothelial EA.hy926 human umbilical cord cells (HUVEC). This effect is dependent on Gla domain of FX. We demonstrate use different mechanisms: Rivaroxaban (RX) specific inhibitor attenuated properties but did not modify...
Abstract Additive effects against tumor cells might be achieved by combining anti‐neoplastic agents directed one or more altered mechanisms in cancer. We investigated the participation of gap junctional intercellular communication (GJIC), which is commonly dysfunctional as a possible mediating mechanism effect all‐trans‐retinoic acid (RA) and tamoxifen (Tx) MCF‐7 human breast cancer cell lines. The combination RA + Tx stimulated GJIC approximately 53 ± 3% early after 6 h treatment remaining...
Fibrinolysis dysfunctions cause bleeding or predisposition to thrombosis. Platelets contain several factors of the fibrinolytic system, which could up down regulate this process. However, temporal relationship and relative contributions plasma platelet components in clot lysis are mostly unknown. We developed a time (CLT) assay platelet-rich (PRP-CLT, with without stimulation) compared it similar one platelet-free (PFP) another previously reported test platelet-poor (PPP). also studied...
<b><i>Background:</i></b> Major depressive disorder (MDD) is an important independent risk factor for cardiovascular disease. Cumulative data suggest that patients exhibit derangement in regional cerebral blood flow (rCBF), although underlying mechanisms remain mostly unknown. Endothelial dysfunction (ED), defined as different forms of abnormal endothelial activity, plays a key role the pathogenesis vascular ED associated with several clinical conditions characterized...
PIEZO1 is a mechanosensitive cation channel implicated in shear stress-mediated endothelial-dependent vasorelaxation. Since altered stress patterns induce pro-inflammatory endothelial environment, we analyzed transcriptional profiles of human cells to determine the effect and subsequent prooxidant inflammatory conditions on mechanosensitive-related genes (MRG). In silico analyses were validated vitro by assessing transcript levels both umbilical artery (HUAEC) vein (HUVEC) endothelium....