A5048805238- Zebrafish Biomedical Research Applications
- Developmental Biology and Gene Regulation
- Aquaculture disease management and microbiota
- Congenital heart defects research
- RNA Research and Splicing
- Cancer-related gene regulation
- Genomics and Phylogenetic Studies
- Animal Genetics and Reproduction
- Wnt/β-catenin signaling in development and cancer
- Fish biology, ecology, and behavior
- Hearing, Cochlea, Tinnitus, Genetics
- Retinal Development and Disorders
- Nerve injury and regeneration
- Axon Guidance and Neuronal Signaling
- Fish Biology and Ecology Studies
- MicroRNA in disease regulation
- Marine animal studies overview
- Immune Response and Inflammation
- Vibrio bacteria research studies
- RNA modifications and cancer
- Physiological and biochemical adaptations
- Genomics and Chromatin Dynamics
- Pluripotent Stem Cells Research
- Neurogenesis and neuroplasticity mechanisms
- Epigenetics and DNA Methylation
University of Chile
2016-2025
Millennium Institute for Integrative Biology
2022-2025
Millennium Science Initiative
2004-2024
Millennium Institute
2023-2024
Organization of American States
2020
United Nations
2020
National Institutes of Health
2020
UNESCO
2020
Rockefeller University
2020
University of California, Davis
2017-2018
ABSTRACT We describe the isolation of zebrafish MyoD gene and its expression in wild-type embryos two mutants with altered somite development, no tail (ntl) spadetail (spt). In embryo, first occurs an early phase, extending from mid-gastrula to just prior formation, which cells directly adjacent axial mesoderm express gene. subsequent phases, during anterior-to-posterior wave formation maturation, within particular regions each somite. spt embryos, lack normal paraxial due incorrect cell...
Somitogenesis is the basis of segmentation mesoderm in trunk and tail vertebrate embryos. Two groups mutants with defects this patterning process have been isolated our screen for zygotic mutations affecting embryonic development zebrafish (Danio rerio). In first group, boundaries between individual somites are invisible early on, although paraxial present. Later, irregular Mutations fused (fss) beamter (bea) affect all somites, whereas deadly seven (des), after eight (aei) white (wit) only...
November 2020 marked 2 y since the launch of Earth BioGenome Project (EBP), which aims to sequence all known eukaryotic species in a 10-y timeframe. Since then, significant progress has been made across aspects EBP roadmap, as outlined 2018 article describing project’s goals, strategies, and challenges (1). The phase ended clock started on reaching EBP’s major milestones. This Special Feature explores many facets EBP, including review progress, description scientific exemplar projects,...
In a large scale screen for mutants with defects in the embryonic development of zebrafish we identified mutations four genes,floating head (flh), momo (mom), no tail (ntl), and doc, that are required early notochord formation. Mutations flh ntl have been described previously, while mom doc newly genes. Mutant embryos lack trunk, trunk somites from right left side embryo fuse underneath neural tube. this respect appears similar to flh. contrast, precursor cells present both embryos. order...
Recent studies of the genome architecture vertebrates have uncovered two unforeseen aspects its organization. First, large regions genome, called gene deserts, are devoid protein-coding sequences and no obvious biological role. Second, comparative genomics has highlighted existence an array highly conserved non-coding (HCNRs) in all vertebrates. Most surprisingly, these structural features strongly associated with genes that essential functions during development. Among these, vertebrate...
Recently our laboratory described an efficient method for generating retroviral provirus insertions in the zebrafish germ line, and we showed that induce embryonic mutations at a frequency of roughly one mutant per 70 insertions. To date have isolated four insertional mutants and, using proviruses as molecular tag, cloned genes disrupted three them. The all lie within or just 5' first coding exon, point opposite transcriptional orientation from gene, disrupt transcription. Here present...
Abstract Background Studies on innate immunity have benefited from the introduction of zebrafish as a model system. Transgenic fish expressing fluorescent proteins in leukocyte populations allow direct, quantitative visualization an inflammatory response vivo . It has been proposed that this animal can be used for high-throughput screens aimed at identification novel immunomodulatory lead compounds. However, current assays require invasive manipulation individually, thus preventing...
Abstract Mechanosensory hair cells are essential for audition in vertebrates, and many species, have the capacity regeneration when damaged. Regeneration is robust fish lateral line system as new can reappear after damage induced by waterborne aminoglycoside antibiotics, platinum‐based drugs, heavy metals. Here, we characterize loss reappearance of zebrafish larvae treated with copper sulfate using diverse molecular markers. Transgenic that express green fluorescent protein different cell...
The role of macrophages during regeneration in zebrafish has been well documented. Nevertheless, new evidence indicates that are a heterogeneous population cells, and they can play different roles immune responses tissue restoration after damage infection. In this work, we first aimed to classify according their distribution the larva homeostasis damage, distinguishing peripheral hematopoietic resident macrophages. We discovered differences between migratory behavior these two macrophage...
The Atacama Desert in Chile-hyperarid and with high-ultraviolet irradiance levels-is one of the harshest environments on Earth. Yet, dozens species grow there, including Atacama-endemic plants. Herein, we establish Talabre-Lejía transect (TLT) as an unparalleled natural laboratory to study plant adaptation extreme environmental conditions. We characterized climate, soil, plant, soil-microbe diversity at 22 sites (every 100 m altitude) along TLT over a 10-y period. quantified drought,...
Two achaete-scute homolog sequences, Zash-1a and Zash-1b , were isolated from a zebrafish embryonic cDNA library. The encodes protein very similar to rat Mash-1 Xenopus Xash-1, with over 94% identity in the C-terminal three-fourths of all three polypeptides. more distantly related protein, 80% amino acids this part sequence. At 24 hr, transcripts are found hindbrain two bilaterally symmetrical lines cells which mark boundary between alar basal plates rhombomere 1 ventral near floorplate....
An important technology in model organisms is the ability to make transgenic animals. In past, zebrafish has been limited by relatively low efficiency with which transgenes could be generated using either DNA microinjection or retroviral infection. Previous efforts generate vectors used a pseudotyped virus genome based on Moloney murine leukemia and envelope protein of vesicular stomatitis virus. This was injected into blastula-stage zebrafish, 16% embryos transmitted proviral insertions...
Abstract Background Understanding the cellular mechanisms regulating axon degeneration and regeneration is crucial for developing treatments nerve injury neurodegenerative disease. In neurons, distinct from cell body death often precedes or associated with onset of disease symptoms. peripheral nervous system both vertebrates invertebrates, after detached fragments, axons can regenerate to restore function. Many studies axonal have used in vitro approaches, but influence extrinsic types on...
In animals, hatching represents the transition point from a developing embryo to free-living individual, larva. This process is finely regulated by many endogenous and environmental factors has been shown be sensitive variety of chemical agents. It commonly evaluated in bioassays order establish effects different agents on early development reproductive capabilities fish other aquatic animals. fish, breakdown chorion achieved secretion choriolysin gland cells (HGCs) into perivitelline space...
Transcription factor Runx2 controls bone development and osteoblast differentiation by regulating expression of a significant number bone-related target genes. Here, we report that transcriptional activation repression the gene via its osteoblast-specific P1 promoter (encoding mRNA for Runx2/p57 isoform) is accompanied selective deposition elimination histone marks during mesenchymal cells to osteogenic myoblastic lineages. These epigenetic profiles are mediated key components...