A5104992615- Fungal and yeast genetics research
- Microtubule and mitosis dynamics
- Microbial Metabolic Engineering and Bioproduction
- DNA Repair Mechanisms
- Gene Regulatory Network Analysis
- Telomeres, Telomerase, and Senescence
- Environmental DNA in Biodiversity Studies
- CRISPR and Genetic Engineering
- Liver Disease Diagnosis and Treatment
- Slime Mold and Myxomycetes Research
- Cancer-related Molecular Pathways
- Animal Genetics and Reproduction
- Bird parasitology and diseases
- Species Distribution and Climate Change
- Bioinformatics and Genomic Networks
- Hearing, Cochlea, Tinnitus, Genetics
- RNA and protein synthesis mechanisms
- Bacteriophages and microbial interactions
- Cardiovascular Disease and Adiposity
- Nonlinear Dynamics and Pattern Formation
- Spine and Intervertebral Disc Pathology
- Thyroid Disorders and Treatments
- Genetically Modified Organisms Research
- Zoonotic diseases and public health
- Carcinogens and Genotoxicity Assessment
Revive & Restore
2021-2022
Bay Area Discovery Museum
2021-2022
Booz Allen Hamilton (United States)
2018
Paracelsus Medical University
2015
Luzerner Kantonsspital
2015
University of California, San Diego
2013
University of Veterinary Medicine Vienna
2010
Baxter (United States)
2010
Bioengineering Center
2008
Baylor College of Medicine
2003-2005
To initiate studies on how protein-protein interaction (or "interactome") networks relate to multicellular functions, we have mapped a large fraction of the Caenorhabditis elegans interactome network. Starting with subset metazoan-specific proteins, more than 4000 interactions were identified from high-throughput, yeast two-hybrid (HT=Y2H) screens. Independent coaffinity purification assays experimentally validated overall quality this Y2H data set. Together already described and interologs...
November 2020 marked 2 y since the launch of Earth BioGenome Project (EBP), which aims to sequence all known eukaryotic species in a 10-y timeframe. Since then, significant progress has been made across aspects EBP roadmap, as outlined 2018 article describing project’s goals, strategies, and challenges (1). The phase ended clock started on reaching EBP’s major milestones. This Special Feature explores many facets EBP, including review progress, description scientific exemplar projects,...
Meiotic recombination differs from mitotic in that DSBs are repaired using homologous chromosomes, rather than sister chromatids. This change partner choice is due part to a barrier chromatid repair (BSCR) created by the meiosis-specific kinase, Mek1, complex with two other proteins, Hop1 and Red1. HOP1 contains functional domains, called N C domains. Analysis of point mutation specifically inactivates domain (hop1-K593A) reveals sufficient for localization chromosomes Red1 interactions. The...
In yeast, HOP1 and RED1 are required during meiosis for proper chromosome segregation the consequent formation of viable spores. Mutations in either orRED1 create unique as well overlapping phenotypes, indicating that two proteins act alone concert with each other. To understand which meiotic processes specifically require Red1p-Hop1p hetero-oligomers, a novel genetic screen was used to identify single-point mutation RED1,red1-K348E, separates Hop1p binding from Red1p homo-oligomerization....
Cells have evolved complex regulatory networks that reorganize gene expression patterns in response to changing environmental conditions. These changes often involve redundant mechanisms affect various levels of expression. Here, we examine the consequences enhanced mRNA degradation galactose utilization network Saccharomyces cerevisiae . We observe glucose-induced GAL1 transcripts provides a transient growth advantage cells upon addition glucose. show arises from relief translational...
Most yeast genes are dispensable for optimal growth in laboratory cultures. However, this apparent lack of fitness contribution is difficult to reconcile with the theory natural selection. Here we use stochastic modeling show that environmental fluctuations can select a genetic mechanism does not affect static environments. We then present novel experimental platform measuring levels specific genotypes fluctuating test by monitoring mixed culture two strains differ their ability respond...
The yeast metabolic cycle (YMC) is a fascinating example of biological organization, in which cells constrain the function specific genetic, protein and networks to precise temporal windows as they grow divide. However, understanding intracellular origins YMC remains challenging goal, measuring oxygen oscillations traditionally associated with it requires use synchronized cultures growing nutrient-limited chemostat environments. To address these limitations, we used custom-built microfluidic...
As the biodiversity crisis accelerates, stakes are higher for threatened plants and animals. Rebuilding health of our planet will require addressing underlying threats at many scales, including habitat loss climate change. Conservation interventions such as protection, management, restoration, predator control, translocation, genetic rescue, biological control have potential to help or endangered species avert extinction. These existing, well-tested methods can be complemented augmented by...
The tail cut bleeding model (CUT) is routinely used in factor VIII-deficient mice to assess pharmacodynamic effects of therapeutic strategies for haemophilia A. Results from this are highly variable, many modifications the reported and at times animals' wellbeing may be compromised by recording survival as an endpoint. We therefore investigated if ferric chloride carotid occlusion (COM) thrombosis research can applied enhance data quality animal welfare A research. Relative dose relative...
Abstract In June 2020, Revive & Restore hosted the Intended Consequences Workshop to explore how field of conservation can realize benefits genetic interventions and address concerns about unintended consequences these actions. A group 57 participants from eight countries representing government, academia, practice discussed implementation designed achieve objectives be optimized both risk intended consequences. Genetic are efforts manipulate composition a target at species or population...
Acute phase is characterized not only by increased serum levels of acute reactants such as the well studied IL-1β, IL-6 or TNFα, but also reduced thyroid hormone levels. The aim this study was to analyze expression key members signalling pathway TRα1, TRα2, TRβ1, NCoR-1 and newly identified receptor cofactor DOR/TP53INP2 during response (APR) in rat liver.