A5008950622- Genetics, Aging, and Longevity in Model Organisms
- Protein Kinase Regulation and GTPase Signaling
- Enzyme Structure and Function
- Microbial Metabolic Engineering and Bioproduction
- 3D Printing in Biomedical Research
- Cancer Genomics and Diagnostics
- Bioinformatics and Genomic Networks
- Genomics and Phylogenetic Studies
- RNA and protein synthesis mechanisms
- GABA and Rice Research
- Cancer Cells and Metastasis
- Chronic Lymphocytic Leukemia Research
- RNA Research and Splicing
- Genomics and Chromatin Dynamics
- Ubiquitin and proteasome pathways
- Endoplasmic Reticulum Stress and Disease
- Glycosylation and Glycoproteins Research
- Chemical Synthesis and Analysis
- Fungal and yeast genetics research
- DNA Repair Mechanisms
- CRISPR and Genetic Engineering
- Biochemical and Molecular Research
- PI3K/AKT/mTOR signaling in cancer
- 14-3-3 protein interactions
- Agriculture Sustainability and Environmental Impact
MODUL University Dubai
2003-2014
Modul-Bio (France)
2010
Harvard University
2001-2004
Dana-Farber Cancer Institute
2001-2004
Institut de Génétique Moléculaire de Montpellier
2004
University of Namur
2004
Baylor College of Medicine
2004
Harvard University Press
2004
Institut de Génétique Humaine
2003
Granta Design (United Kingdom)
2003
To initiate studies on how protein-protein interaction (or "interactome") networks relate to multicellular functions, we have mapped a large fraction of the Caenorhabditis elegans interactome network. Starting with subset metazoan-specific proteins, more than 4000 interactions were identified from high-throughput, yeast two-hybrid (HT=Y2H) screens. Independent coaffinity purification assays experimentally validated overall quality this Y2H data set. Together already described and interologs...
Protein interaction maps have provided insight into the relationships among predicted proteins of model organisms for which a genome sequence is available. These been useful in generating potential networks, confirmed existence known complexes and pathways suggested new or crosstalk between previously unlinked pathways. However, generation such costly labor intensive. Here, we investigate extent to protein map generated one species can be used predict interactions another species.
Many human cancers originate from defects in the DNA damage response (DDR). Although much is known about this process, it likely that additional DDR genes remain to be discovered. To identify such genes, we used a strategy combines protein-protein interaction mapping and large-scale phenotypic analysis Caenorhabditis elegans. Together, these approaches identified 12 worm orthologs 11 novel genes. One of putative ortholog hBCL3, gene frequently altered chronic lymphocytic leukemia. Thus,...
Five mutants of protein kinase CK2 α subunit in which altogether 14 basic residues were singly to quadruply replaced by alanines (K74A,K75A,K76A,K77A; K79A, R80A,K83A; R191A,R195A,K198A; R228A; and R278A, K279A,R280A) have been purified near homogeneity either as such or after addition the recombinant β subunit. By this latter procedure five mutated tetrameric holoenzymes obtained judged from their composition, sedimentation coefficient on sucrose gradient ultracentrifugation, increased...
Proteome-scale studies of protein three-dimensional structures should provide valuable information for both investigating basic biology and developing therapeutics. Critical these endeavors is the expression recombinant proteins. We selected Caenorhabditis elegans as our model organism in a structural proteomics initiative because high quality its genome sequence availability ORFeome, protein-encoding open reading frames (ORFs), flexible recombinational cloning format. developed robotic...
In view of the common regulatory mechanism that induces transcription mitotic phosphatase cdc25C and cyclin A at beginning S-phase, we investigated whether was required for S-phase transit. Here, show in both nontransformed human fibroblasts HeLa cells, protein levels significantly increased concomitant with onset synthesis. Activity measurements on immunoprecipitates from synchronized cells revealed a sharp rise cdc25C-associated activity coincided S-phase. Microinjection various...
Large collections of protein-encoding open reading frames (ORFs) established in a versatile recombination-based cloning system have been instrumental to study protein functions high-throughput assays. Such 'ORFeome' resources developed for several organisms but virology, plasmid covering significant fraction the virosphere are still needed. In this perspective, we present ViralORFeome 1.0 (http://www.viralorfeome.com), an open-access database and management that provides integrated set...
Sixteen derivatives of the optimal peptide substrate RRRA-DDSDDDDD in which aspartic acids were singly or multiply substituted by alanine have been assayed for their phosphorylation efficiency either wild type protein kinase CK2 α mutants defective recognition. With CK2, only detrimental single substitutions those at positions +3 and +1. Each these caused a 5-fold increase Km 2-fold decrease Vmax values. If both n + 1 3 however, rose 24-fold decreased 16-fold. Multiple tend to more than...
Abstract Background Transcription regulatory networks are composed of protein-DNA interactions between transcription factors and their target genes. A long-term goal in genome biology is to map interaction all regions a interest. Both factor -and gene-centered methods can be used systematically identify such interactions. We use high-throughput yeast one-hybrid assays as method sequences ( e.g . gene promoters) the nematode Caenorhabditis elegans have already mapped several hundred analyzed...
WorfDB (Worm ORFeome DataBase; http://worfdb.dfci.harvard.edu) was created to integrate and disseminate the data from cloning of complete set approximately 19 000 predicted protein-encoding Open Reading Frames (ORFs) Caenorhabditis elegans (also referred as 'worm ORFeome'). serves a central repository enabling scientific community search for availability quality cloned ORFs. So far, ORF sequence tags (OSTs) obtained all individual clones have allowed exon structure corrections 3400 ORFs...
The quadruple mutation of the whole basic cluster, K74KKK77 conserved in catalytic subunits protein kinase CK2 and implicated substrate recognition, not only abolishes inhibition by heparin but even induces with some peptide substrates an up to 5-fold stimulation 0.5-5 micrograms/ml concentration range. Two other mutants defective R191, 195K198A K79R80K83A, display either a 100-fold reduction or no alteration at all inhibition, respectively. In contrast sensitivity is increased 30-fold...
Protein kinase CK2 is a ubiquitous pleiotropic serine/threonine protein whose holoenzyme comprised of two catalytic (α and/or α') and non‐catalytic, β‐subunits. The β‐subunit possesses antagonist functions that can be physically dissected by generating synthetic fragments encompassing its N‐terminal C‐terminal domains. Here we show mutating basic residues in the 74‐77 191‐198 regions α‐subunit, negative regulation fragment CK2β‐(1–77), which observable using calmodulin as substrate for...
An increasing number of laboratories are using the COPAS Biosort™ to implement high-throughput approaches tackle diverse biological problems. While providing a powerful tool for generating quantitative data, utility Biosort is currently limited by absence resources data management. We describe simple electronic database designed allow easy storage and retrieval C. elegans, but that has wide potential application organizing files sets. ICeE an Open Source application. The code accompanying...