A5063071277- Hepatitis C virus research
- Hepatitis B Virus Studies
- Immunotherapy and Immune Responses
- T-cell and B-cell Immunology
- Monoclonal and Polyclonal Antibodies Research
- Mosquito-borne diseases and control
- Immune Cell Function and Interaction
- Bioinformatics and Genomic Networks
- Immune Response and Inflammation
- interferon and immune responses
- Liver Disease Diagnosis and Treatment
- Influenza Virus Research Studies
- Systemic Lupus Erythematosus Research
- RNA and protein synthesis mechanisms
- Immunodeficiency and Autoimmune Disorders
- Endoplasmic Reticulum Stress and Disease
- Viral Infections and Vectors
- HIV Research and Treatment
- Cancer, Hypoxia, and Metabolism
- Cholesterol and Lipid Metabolism
- Drug Transport and Resistance Mechanisms
- Biochemical and Molecular Research
- Atherosclerosis and Cardiovascular Diseases
- SARS-CoV-2 and COVID-19 Research
- Immune cells in cancer
Centre International de Recherche en Infectiologie
2015-2024
Université Claude Bernard Lyon 1
2015-2024
École Normale Supérieure de Lyon
2014-2024
Inserm
2014-2024
Centre National de la Recherche Scientifique
2014-2024
Le Laboratoire P4 Inserm Jean Mérieux
2024
Biology of Infection
2019
Hospices Civils de Lyon
2007-2018
University of Illinois Chicago
2017
Centre National pour la Recherche Scientifique et Technique (CNRST)
2013
ABSTRACT The presence of hepatitis C virus (HCV) RNA-containing particles in the low-density fractions plasma has been associated with high infectivity. However, nature circulating HCV and their association immunoglobulins or lipoproteins as well characterization cell entry have all subject to conflicting reports. For a better analysis particles, we quantified RNA corresponding very-low-density lipoprotein (VLDL), intermediate-density lipoprotein, (LDL) from untreated chronically...
A proteome-wide mapping of interactions between hepatitis C virus (HCV) and human proteins was performed to provide a comprehensive view the cellular infection. total 314 protein-protein HCV identified by yeast two-hybrid 170 literature mining. Integration this data set into reconstructed interactome showed that interacting with are enriched in highly central interconnected proteins. global analysis on basis functional annotation highlighted enrichment pathways targeted HCV. network...
Inhibitors of the protease HIV-1 have been used successfully for treatment HIV-1-infected patients and AIDS disease. We tested whether these inhibitory drugs exerted effects in addition to their antiviral activity. Here, we show mice infected with lymphocytic choriomeningitis virus treated inhibitor ritonavir a marked inhibition cytotoxic T lymphocyte (CTL) activity impaired major histocompatibility complex class I-restricted epitope presentation absence direct on replication. A potential...
Autophagy is a conserved degradative pathway used as host defense mechanism against intracellular pathogens. However, several viruses can evade or subvert autophagy to insure their own replication. Nevertheless, the molecular details of viral interaction with remain largely unknown. We have determined ability 83 proteins families RNA (Paramyxoviridae, Flaviviridae, Orthomyxoviridae, Retroviridae and Togaviridae), interact 44 human autophagy-associated using yeast two-hybrid bioinformatic...
Infectious diseases caused by viral agents kill millions of people every year. The improvement prevention and treatment infections their associated remains one the main public health challenges. Towards this goal, deciphering virus–host molecular interactions opens new perspectives to understand biology infection for design antiviral strategies. Indeed, modelling an network between cellular proteins will provide a conceptual analytic framework efficiently formulate biological hypothesis at...
Abstract Dysregulated Toll-like receptor (TLR)-4 activation is involved in acute systemic sepsis, chronic inflammatory diseases, such as atherosclerosis and diabetes, viral infections, influenza infection. Thus, therapeutic control of the TLR4 signalling pathway major interest. Here we tested activity small-molecule synthetic antagonist, FP7, vitro on human monocytes monocyte-derived dendritic cells (DCs) vivo during virus infection mice. Our results indicate that FP7 antagonized secretion...
Chikungunya virus (CHIKV) is a mosquito-transmitted alphavirus that has been responsible for an epidemic outbreak of unprecedented magnitude in recent years. Since then, significant efforts have made to better understand the biology this virus, but we still poor knowledge CHIKV interactions with host cell components at molecular level. Here describe extensive use high-throughput yeast two-hybrid (HT-Y2H) assays characterize between and human proteins. A total 22 high-confidence interactions,...
ABSTRACT The study of cellular central carbon metabolism modulations induced by viruses is an emerging field. Human cytomegalovirus (HCMV), herpes simplex virus (HSV), Kaposi's sarcoma-associated herpesvirus (KSHV), and hepatitis C (HCV) have been shown recently to reprogram cell support their replication. During HCV infection the global glucidolipidic hepatocytes highly impacted. It was suggested that might modify glucose uptake glycolysis increase fatty acids synthesis, but underlying...
Influenza A NS1 and NS2 proteins are encoded by the RNA segment 8 of viral genome. is a multifunctional protein virulence factor while involved in nuclear export ribonucleoprotein complexes. yeast two-hybrid screening strategy was used to identify host factors supporting functions. More than 560 interactions between 79 cellular from 9 different influenza virus strains have been identified. These interacting potentially each step infectious process their contribution replication tested...
Abstract The current therapeutic arsenal against viral infections remains limited, with often poor efficacy and incomplete coverage, appears inadequate to face the emergence of drug resistance. Our understanding biology pathophysiology our ability develop a more effective antiviral would greatly benefit from comprehensive picture events that lead replication associated symptoms. Towards this goal, construction virus-host interactomes is instrumental, mainly relying on assumption infection at...
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the causative agent of COVID-19 pandemic, which has led to a devastating global health crisis. The emergence variants that escape neutralizing responses emphasizes urgent need deepen our understanding SARS-CoV-2 biology. Using comprehensive identification RNA-binding proteins (RBPs) by mass spectrometry (ChIRP-MS) approach, we identify 107 high-confidence cellular factors interact with genome during infection. By systematically...
The density of circulating hepatitis C virus (HCV) particles in the blood chronically infected patients is very heterogeneous. low some has been attributed to an association with apolipoprotein B (apoB) positive and triglyceride rich lipoproteins (TRL) likely resulting hybrid known as lipo-viro-particles (LVP) containing viral envelope glycoproteins E1 E2, capsid RNA. specific infectivity these shown be higher than density. nature HCV remains elusive role apolipoproteins synthesis assembly...
Abstract Background The genus Flavivirus encompasses more than 50 distinct species of arthropod-borne viruses, including several major human pathogens, such as West Nile virus, yellow fever Japanese encephalitis virus and the four serotypes dengue viruses (DENV type 1-4). Each year, flaviviruses cause 100 million infections worldwide, some which lead to life-threatening conditions or haemorrhagic fever. Among viral proteins, NS3 NS5 proteins constitute enzymatic components replication...
Identification of new techniques to express proteins into mammal cells is particular interest for both research and medical purposes. The present study describes the use engineered vesicles deliver exogenous human cells. We show that overexpression spike glycoprotein vesicular stomatitis virus (VSV-G) in induces release fusogenic named gesicles. Biochemical functional studies revealed gesicles incorporated from producer could them recipient This protein-transduction method allows direct...
Comprehensive understanding of molecular mechanisms underlying viral infection is a major challenge towards the discovery new antiviral drugs and susceptibility factors human diseases. New advances in field are expected from systems-level modelling integration incessant torrent high-throughput "-omics" data. Here, we describe Human Infectome protein interaction Network, novel systems virology model virtual virus-infected cell concerning 110 viruses. This silico was applied to comprehensively...
Marburg virus (MARV) has a high fatality rate in humans, causing hemorrhagic fever characterized by massive viral replication and dysregulated inflammation. Here, we demonstrate that VP24 of MARV binds Kelch-like ECH-associated protein 1 (Keap1), negative regulator nuclear transcription factor erythroid-derived 2 (Nrf2). Binding to Keap1 Kelch domain releases Nrf2 from Keap1-mediated inhibition promoting persistent activation panoply cytoprotective genes implicated cellular responses...