A5044934855- T-cell and Retrovirus Studies
- Animal Disease Management and Epidemiology
- Vector-Borne Animal Diseases
- Mosquito-borne diseases and control
- Viral Infections and Vectors
- SARS-CoV-2 and COVID-19 Research
- Phagocytosis and Immune Regulation
- Viral Infections and Outbreaks Research
- Herpesvirus Infections and Treatments
- Complement system in diseases
- HIV Research and Treatment
- interferon and immune responses
- RNA Research and Splicing
- CRISPR and Genetic Engineering
- Hepatitis B Virus Studies
- Malaria Research and Control
- Hepatitis C virus research
- COVID-19 Clinical Research Studies
- Barrier Structure and Function Studies
- Immune Response and Inflammation
- Insect symbiosis and bacterial influences
- Immunotherapy and Immune Responses
- Inflammasome and immune disorders
- Virus-based gene therapy research
- Liver Disease Diagnosis and Treatment
Hôpital Saint-Louis
2013-2024
Université Paris Cité
2012-2024
Inserm
2013-2024
Centre National de la Recherche Scientifique
2011-2024
Institut de recherche Saint-Louis
2020-2024
Génomes, biologie cellulaire et thérapeutiques
2010-2018
Délégation Paris 7
2012-2017
Sorbonne Paris Cité
2012-2017
Institut Universitaire de France
2016
Institut Pasteur
2000-2011
Dengue viruses (DVs) are responsible for the most medically relevant arboviral diseases. However, molecular interactions mediating DV entry poorly understood. We determined that TIM and TAM proteins, two receptor families mediate phosphatidylserine (PtdSer)-dependent phagocytic removal of apoptotic cells, serve as factors. Cells susceptible to robustly infected after ectopic expression or receptors. Conversely, infection cells is inhibited by anti-TIM anti-TAM antibodies knockdown...
ZIKA virus (ZIKV) is an emerging pathogen responsible for neurological disorders and congenital microcephaly. However, the molecular basis ZIKV neurotropism remains poorly understood. Here, we show that Axl expressed in human microglia astrocytes developing brain it mediates infection of glial cells. Axl-mediated entry requires ligand Gas6, which bridges particles to Following binding, internalized through clathrin-mediated endocytosis traffics Rab5+ endosomes establish productive infection....
Autosomal inborn errors of type I IFN immunity and autoantibodies against these cytokines underlie at least 10% critical COVID-19 pneumonia cases. We report very rare, biochemically deleterious X-linked TLR7 variants in 16 unrelated male individuals aged 7 to 71 years (mean: 36.7 years) from a cohort 1,202 patients 0.5 99 52.9 with unexplained pneumonia. None the 331 asymptomatically or mildly infected 1.3 102 38.7 tested carry such (p = 3.5 × 10-5). The phenotypes five hemizygous relatives...
Several studies have analyzed antiviral immune pathways in late-stage severe COVID-19. However, the initial steps of SARS-CoV-2 immunity are poorly understood. Here we isolated primary viral strains and studied their interaction with human plasmacytoid predendritic cells (pDCs), a key player immunity. We show that pDCs not productively infected by SARS-CoV-2. they efficiently diversified into activated P1-, P2-, P3-pDC effector subsets response to stimulation. They expressed CD80, CD86,...
Hepatitis C virus (HCV) is a major human pathogen associated with life-threatening liver disease. Entry into hepatocytes requires CD81 and putative second receptor. In this study, we elucidated the postreceptor attachment stages of HCV entry using pseudoparticles (HCVpp) as model system. By means dominant-negative mutants short interfering RNAs various cellular proteins, showed that HCVpp enter via clathrin-coated vesicles require delivery to early but not late endosomes. However, kinetics...
Hepatitis C virus (HCV) is a major cause of liver disease in humans. The CD81 tetraspanin necessary but not sufficient for HCV penetration into hepatocytes, and it was recently reported that the tight junction protein claudin-1 critical entry cofactor. Here, we confirm role entry. In addition, show claudin-6 claudin-9 expressed CD81(+) cells also enable pseudoparticles derived from six genotypes. Whereas claudin-1, -6, -9 function equally well as cofactors endothelial cells, more efficient...
Dengue virus (DENV) infections cause the most prevalent mosquito-borne viral disease worldwide, for which no therapies are available. DENV encodes seven non-structural (NS) proteins that co-assemble and recruit poorly characterized host factors to form replication complex essential infection. Here, we provide a global proteomic analysis of human interact with NS1 protein. Combined functional RNAi screen, this study reveals comprehensive network cellular processes involved in infection...
Dengue virus (DENV) is the etiological agent of major human arboviral disease. We previously demonstrated that TIM and TAM families phosphatidylserine (PtdSer) receptors involved in phagocytosis apoptotic cells mediate DENV entry into target cells. show here CD300a, a recently identified phospholipid receptor, also binds directly particles enhances viral entry. CD300a facilitates infection four serotypes, as well other mosquito-borne viruses such West Nile Chikungunya virus. acts an...
Dengue virus (DENV) is a major human pathogen causing millions of infections yearly. Despite intensive investigations, DENV receptor that directly participates in internalization has not yet been characterized. Here, we report the phosphatidylserine TIM-1 an authentic entry plays active role endocytosis. Genetic ablation strongly impaired infection. Total internal reflection fluorescence microscopy analyses live infected cells show mostly confined clathrin-coated pits and co-internalized...
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the causative agent of COVID-19 pandemic, which has led to a devastating global health crisis. The emergence variants that escape neutralizing responses emphasizes urgent need deepen our understanding SARS-CoV-2 biology. Using comprehensive identification RNA-binding proteins (RBPs) by mass spectrometry (ChIRP-MS) approach, we identify 107 high-confidence cellular factors interact with genome during infection. By systematically...
ABSTRACT The live attenuated yellow fever virus (YFV) vaccine 17D stands as a “gold standard” for successful vaccine. was developed empirically by passaging the wild-type Asibi strain in mouse and chicken embryo tissues. Despite its immense success, molecular determinants virulence attenuation immunogenicity of are poorly understood. evolved several mutations genome, most which lie within envelope (E) protein. Given major role played YFV E protein during entry, it has been hypothesized that...
Dengue virus (DENV) is a mosquito-borne flavivirus responsible for dengue disease, major human health concern which no effective treatment available. DENV relies heavily on the host cellular machinery productive infection. Here, we show that scaffold protein RACK1, part of replication complex, mediates infection by binding to 40S ribosomal subunit. Mass spectrometry analysis RACK1 partners coupled an RNA interference screen-identified Vigilin and SERBP1 as host-dependency factors. Both are...
Alphaviruses, such as chikungunya virus (CHIKV), are mosquito-borne viruses that represent a significant threat to human health due the current context of global warming. Efficient alphavirus infection relies on activity non-structural protein 3 (nsP3), puzzling multifunctional molecule whose role in remains largely unknown. NsP3 is component plasma membrane-bound viral RNA replication complex (vRC) essential for amplification and also found large cytoplasmic aggregates unknown function
ABSTRACT Claudin-1, a component of tight junctions between liver hepatocytes, is hepatitis C virus (HCV) late-stage entry cofactor. To investigate the structural and functional roles various claudin-1 domains in HCV entry, we applied mutagenesis strategy. Putative intracellular were not important. However, identified seven novel residues first extracellular loop that are critical for isolates drawn from six different subtypes. Most belong to highly conserved claudin motif W 30 -GLW 51 -C 54...
The ubiquitin ligase CBLL1 (also known as HAKAI) has been proposed to be a critical cellular factor exploited by West Nile virus (WNV) for productive infection. emerged major hit in recent RNA interference screen designed identify factors required the early stages of WNV life cycle. Follow-up experiments showed that HeLa cells knocked down small interfering (siRNA) failed internalize particles and resisted Furthermore, depletion free-ubiquitin pool proteasome inhibitor MG132 abolished...
Dengue virus (DENV) is a mosquito-borne flavivirus responsible for dengue disease, major human health concern which no specific therapies are available. Like other viruses, DENV relies heavily on the host cellular machinery productive infection. In this study, we performed genome-wide CRISPR-Cas9 screen using haploid HAP1 cells to identify genes important We identified DPM1 and -3, two subunits of endoplasmic reticulum (ER) resident dolichol-phosphate mannose synthase (DPMS) complex, as...
Human T-cell leukemia virus type 1 and 2 (HTLV-1/2) are related retroviruses that infect T-lymphocytes. Whereas HTLV-1 infection can cause leukemia, HTLV-2 has not been demonstrated to be the agent of a hematological malignant disease. Nevertheless, virally encoded Tax-1 Tax-2 transactivators display high percentage similarity. is shuttling protein contains noncanonical nuclear localization signal as well export signal. The presence domains in sequence determined. distribution infected cells...
Background. The presence and origin of endemic foci human T-lymphotropic virus type 2 (HTLV2) infection in Africa remain a matter debate.
ABSTRACT To gain insight on the significance of human T-cell lymphotropic virus type 1 (HTLV-1) indeterminate serological reactivities, we studied villagers South Cameroon, focusing a frequent and specific HTLV-1 Gag profile (HGIP) pattern ( gag p19, p26, p28, p30 without p24 or Env gp21 gp46). Among 102 sera studied, 29 from all age groups had stable HGIP over period 4 years. There was no epidemiological evidence for sexual vertical transmission HGIP. Seventy-five percent reacted positively...