A5075852230- Pesticide and Herbicide Environmental Studies
- Adipose Tissue and Metabolism
- Autophagy in Disease and Therapy
- Mechanisms of cancer metastasis
- Pesticide Residue Analysis and Safety
- Cardiovascular Function and Risk Factors
- Pancreatic function and diabetes
- 3D Printing in Biomedical Research
- African Botany and Ecology Studies
- Pluripotent Stem Cells Research
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- RNA modifications and cancer
- Liver physiology and pathology
- Cancer Mechanisms and Therapy
- Inflammasome and immune disorders
- Mitochondrial Function and Pathology
- Immune Cell Function and Interaction
- Agricultural safety and regulations
- Forest Management and Policy
- Insect and Pesticide Research
- Cancer-related molecular mechanisms research
- Wnt/β-catenin signaling in development and cancer
- MicroRNA in disease regulation
- Systemic Sclerosis and Related Diseases
- Immunotherapy and Immune Responses
Inserm
2016-2025
Université Paris Cité
2015-2025
Institut Cochin
2016-2025
Centre National de la Recherche Scientifique
2016-2025
Ifremer
2017-2025
Centre de Recherche sur l'Inflammation
2023
Délégation Paris 5
2012-2019
Sorbonne Paris Cité
2014-2019
Descartes (Belgium)
2012
Abstract Myelodysplastic syndromes (MDS) with mutated SF3B1 gene present features including a favourable outcome distinct from MDS mutations in other splicing factor genes SRSF2 or U2AF1 . Molecular bases of these divergences are poorly understood. Here we find that -mutated show reduced R-loop formation predominating bodies associated intron retention reduction, not found - MDS. Compared to erythroblasts SRSF2- patients, exhibit augmented DNA synthesis, accelerated replication forks, and...
Takotsubo cardiomyopathy is a stress-induced cardiovascular disease with symptoms comparable to those of an acute coronary syndrome but without obstruction. was initially considered spontaneously reversible, epidemiological studies revealed significant long-term morbidity and mortality, the reason for which unknown. Here, we show in female rodent model that single pharmacological challenge creates similar Takotsubo. The response involves changes blood tissue biomarkers cardiac vivo imaging...
Abstract Objectives Pulmonary arterial hypertension (PAH) is a rare disease that may be associated with connective tissue diseases (CTD). Anti-fibroblast (AFA) and anti-endothelial cell autoantibodies (AECA) have been identified in idiopathic systemic sclerosis (SSc)-associated PAH. The aim was to identify discriminating for PAH lupus erythematosus (SLE), mixed (MCTD) primary Sjögren's syndrome (SS), their target antigens. Methods Sera were collected the French multicentre Auto-HTAP study...
Introduction: Neutrophils are key players in the hyperinflammatory response during SARS-CoV-2 infection. The cytosolic proliferating cell nuclear antigen (PCNA) is a scaffolding protein highly dependent on microenvironment status and known to interact with numerous proteins that regulate neutrophil functions. This study aimed examine content PCNA interactome neutrophils from COVID-19 patients. Methods: Proteomic analyses were performed cytosols healthy donors patients severe or critical...
Abstract Excessive glucose production by the liver is a key factor in hyperglycemia observed type 2 diabetes mellitus (T2DM). Here, we highlight novel role of kinase B1 (Lkb1) this regulation. We show that mice with hepatocyte-specific deletion Lkb1 have higher levels hepatic amino acid catabolism, driving gluconeogenesis. This effect during both fasting and postprandial period, identifying as critical suppressor Hepatic associated major changes whole-body metabolism, leading to lower lean...
Abstract Background Mitochondrial nucleoside diphosphate kinase (NDPK-D, NME4, NM23-H4) is a multifunctional enzyme mainly localized in the intermembrane space, bound to inner membrane. Results We constructed loss-of-function mutants of NDPK-D, lacking either NDP activity or membrane interaction and expressed wild-type protein cancer cells. In complementary approach, we performed depletion NDPK-D by RNA interference. Both mutations promoted epithelial-mesenchymal transition increased...
Granulomatosis with polyangiitis (GPA) is an autoimmune vasculitis associated anti-neutrophil-cytoplasmic antibodies (ANCA) against proteinase 3 leading to kidney damage. Neutrophils from those patients have increased expression of membrane during apoptosis. Here we examined whether neutrophils GPA dysregulated protein expressions A global proteomic analysis was performed comparing GPA, healthy individuals under basal conditions and At disease onset, the cytosolic proteome before treatment...
Abstract Endothelial cells (ECs) are constantly submitted in vivo to hemodynamical forces derived from the blood circulation, including shear stress (SS). ECs able detect SS and consequently adapt their phenotype, thus affecting many endothelial functions. If a plethora of stress-regulated molecular networks have been described peripheral ECs, less is known about responses microvascular brain which constitute blood–brain barrier (BBB). In this work, we investigated response human cerebral...
Abstract Cytosolic proliferating cell nuclear antigen (PCNA) is involved in neutrophil survival and function, which it acts as a scaffold associates with proteins apoptosis, NADPH oxidase activation, cytoskeletal dynamics, metabolism. While the PCNA interactome has been characterized neutrophils under homeostatic conditions, less known about pathophysiological contexts. Granulocyte colony-stimulating factor (G-CSF) cytokine produced response to inflammatory stimuli that regulates many...
Foxo family transcription factors are critically involved in multiple processes, such as metabolism, quiescence, cell survival and differentiation. Although continuous, high activity of extends the life span some species, involvement proteins mammalian aging remains to be determined. Here, we show that Foxo1 is down-regulated with age mouse T cells. This down-regulation cells may contribute disruption naive T-cell homeostasis age, leading an increase number memory also associated...
Psoriasis is a multifactorial, chronic inflammatory skin disease with unresolved questions on its primary events. Iron overload has been described in the epidermis of psoriasis patients, but relevance remains unknown. We found that key iron regulatory hormone hepcidin was highly expressed especially pustular variants resistant to treatments. In murine model acute inflammation, keratinocyte-derived required for retention keratinocytes, leading hyperproliferation epidermal layer and neutrophil...
Abstract Dysregulated autophagy is associated with cardiovascular and metabolic diseases, where impaired flow-mediated endothelial cell responses promote risk. The mechanism by which the machinery regulates functions complex. We applied multi-omics approaches in vitro vivo functional assays to decipher diverse roles of cells. demonstrate that VEGF-dependent VEGFR signaling VEGFR-mediated eNOS activation. Endothelial ATG5 deficiency results selective loss flow-induced vasodilation mesenteric...
Human umbilical vein endothelial cells (HUVEC) are widely used as a source of (EC). However, HUVEC characteristics cannot be extrapolated to other types EC, particularly microvascular ECs. Our objective was compare the proteomes ECs and HUVEC. Proteomes human pulmonary EC (HMVEC‐P) dermal (HMVEC‐D) from healthy Caucasian donors were compared by 2D DIGE MS. Fatty acid binding proteins 4 5 among 159 30 spots found have at least twofold change in expression between HMVEC‐D HMVEC‐P samples,...
Axin1 is a negative regulator of wingless-type MMTV integration site family, member 1 (Wnt)/β-catenin signaling with tumor-suppressor function. The Wnt pathway has critical role in the intestine, both during homeostasis and cancer, but remains elusive.We assessed normal intestinal homeostasis, control, epithelial-specific, Axin1-knockout mice (Axin1ΔIEC) Axin2-knockout mice. We evaluated function chemically induced colorectal tumorigenesis dextran sulfate sodium-induced colitis, performed...
Abstract Cancer cells rely on mitochondria for their bioenergetic supply and macromolecule synthesis. Central to mitochondrial function is the regulation of protein synthesis, which primarily depends cytoplasmic translation nuclear-encoded mRNAs whose products are imported into mitochondria. Despite growing evidence that synthesis contributes onset progression cancer, can thus offer new opportunities cancer therapy, knowledge underlying molecular mechanisms remains limited. Here, we show RNA...
Hepatitis E virus (HEV) causes acute enterically transmitted hepatitis. In industrialized countries, it is a zoonotic disease, with swine being the major reservoir of human HEV contamination. The occurrence and severity disease are variable, clinical symptoms ranging from asymptomatic to self-limiting hepatitis, chronic infection, or fulminant absence robust cell culture system small-animal models, life cycle pathological process remain unclear. To characterize pathogenesis virulence...