A5031278146- CAR-T cell therapy research
- Cellular Mechanics and Interactions
- Wnt/β-catenin signaling in development and cancer
- Primary Care and Health Outcomes
- Interprofessional Education and Collaboration
- Cell Adhesion Molecules Research
- Cancer Immunotherapy and Biomarkers
- Histone Deacetylase Inhibitors Research
- Cancer Mechanisms and Therapy
- Chronic Disease Management Strategies
- Healthcare Systems and Technology
- Cancer, Hypoxia, and Metabolism
- Multiple Myeloma Research and Treatments
- Cancer-related gene regulation
- Mental Health and Patient Involvement
- 3D Printing in Biomedical Research
- Health Policy Implementation Science
- Metabolism, Diabetes, and Cancer
- Force Microscopy Techniques and Applications
- Acute Myeloid Leukemia Research
- PI3K/AKT/mTOR signaling in cancer
- Axon Guidance and Neuronal Signaling
- Receptor Mechanisms and Signaling
- Patient Satisfaction in Healthcare
- Advanced Materials and Mechanics
Université Paris Cité
2002-2024
Inserm
2010-2024
Institut Cochin
2013-2024
Centre National de la Recherche Scientifique
2010-2024
Université de Sherbrooke
2023-2024
La Ligue Contre le Cancer
2009-2022
Joint Research Center
2021
Centre de Recherche Jean Pierre Aubert
2019
Sorbonne Université
2002-2017
Délégation Paris 5
2009-2017
Mechanical forces play an important role in the organization, growth and function of living tissues. The ability cells to transduce mechanical signals is governed by two types microscale structures: focal adhesions, which link extracellular matrix, adherens junctions, adjacent through cadherins. Although many studies have examined induced there little known about junctions force-regulation processes. present study focuses on determination force transduction cadherins at a single cell level....
The adhesion molecule N-cadherin plays important roles in the development of nervous system, particular by stimulating axon outgrowth, but molecular mechanisms underlying this effect are mostly unknown. One possibility, so-called "molecular clutch" model, could involve a direct mechanical linkage between at membrane and intracellular actin-based motility within neuronal growth cones. Using live imaging primary rat hippocampal neurons plated on N-cadherin-coated substrates optical trapping...
Background Reports of reduced pain sensitivity in autism have prompted opioid theories and practical care ramifications. Our objective was to examine behavioral physiological responses, plasma β-endorphin levels their relationship a large group individuals with autism. Methodology/Principal Findings The study conducted on 73 children adolescents 115 normal matched for age, sex pubertal stage. Behavioral reactivity assessed three observational situations (parents at home, two caregivers...
<h3>PURPOSE</h3> Although case management (CM) is increasingly being implemented to address the complex needs of vulnerable clienteles, few studies have examined patient experience CM. This study aimed examine patients and their family members with care integration as part a primary CM intervention. Patients in were frequent users health services who had chronic diseases. <h3>METHODS</h3> A descriptive, qualitative approach was conducted involving 25 8 members. Data collected through...
AMPK is a master regulator of cellular metabolism that exerts either oncogenic or tumor suppressor activity depending on context. Here, we report the specific agonist GSK621 selectively kills acute myeloid leukemia (AML) cells but spares normal hematopoietic progenitors. This differential sensitivity results from unique synthetic lethal interaction involving concurrent activation and mTORC1. Strikingly, lethality in primary AML cell lines abrogated by chemical genetic ablation mTORC1...
ABSTRACT Cell adhesion receptors of the cadherin family are involved in various developmental processes, affecting cell and migration, also proliferation differentiation. In order to dissect molecular mechanisms cadherin-based cell-cell subsequent signal transduction cytoskeleton and/or cytoplasm leading adapted responses, we developed an approach allowing us mimic control activation. We produced a dimeric N-cadherin-Fc chimera (Ncad-Fc) which retains structural functional properties...
Cell adhesion molecules of the cadherin family contribute to regulation cell shape and fate by mediating strong intercellular through Ca2+-dependent interaction their ectodomain association cytoplasmic tail actin. However, mechanisms co-ordinating cadherinmediated with reorganization actin cytoskeleton remain elusive. Here, formation de novo contacts was dissected spreading cells on a highly active N-cadherin homophilic ligand. Cells responded activation extending lamellipodium organizing...
Fms-like tyrosine kinase 3 internal tandem duplication (FLT3-ITD) is frequently detected in acute myeloid leukemia (AML) patients and associated with a dismal long-term prognosis. FLT3 inhibitors provide short-term disease control, but relapse invariably occurs within months. Pim protein kinases are oncogenic FLT3-ITD targets expressed AML cells. We show that increased expression found samples from treated inhibitors. Ectopic Pim-2 induces resistance to inhibition both FLT3-ITD-induced...
AMP-activated protein kinase (AMPK) regulates the balance between cellular anabolism and catabolism dependent on energy resources to maintain proliferation survival. Small-compound AMPK activators show anti-cancer activity in preclinical models. Using direct activator GSK621, we that unfolded response (UPR) is activated by acute myeloid leukemia (AML) cells. Mechanistically, UPR effector RNA-like ER (PERK) represses oxidative phosphorylation, tricarboxylic acid (TCA) cycle, pyrimidine...
Cadherin receptors are key morphoregulatory molecules during development. To dissect their mode of action, we developed an approach based on the use myogenic C2 cells and beads coated with Ncad-Fc ligand, allowing us to mimic cadherin-mediated adhesion. We used optical tweezers video microscopy investigate dynamics N-cadherin anchoring within very first seconds bead–cell contact. The analysis bead movement by single-particle tracking indicated that were freely diffusive in few after binding....
Kainate receptors modulate synaptic transmission by acting either at presynaptic or postsynaptic sites. The precise localization of kainate as well the mechanisms targeting and stabilization these in neurons are largely unknown. We have generated transgenic mice expressing receptor subunit glutamate 6 (GluR6) bearing an extracellular myc epitope (myc-GluR6), forebrain neurons, which it assembles with endogenous subunits. In crossed GluR6-deficient mice, myc-GluR6 efficiently rescues missing...
Growing evidence supports patient engagement (PE) in health implementation research to improve the quality, relevance and uptake of research. However, more guidance is needed plan operationalize PE before throughout process. The aim study was develop a logic model illustrating causal links between context, resources, activities, outcomes impact an programme.The Patient Engagement Health Implementation Research Logic Model (hereafter Model) developed using descriptive qualitative design with...
The calbindin-D9K (CaBP9k) gene is mainly expressed in differentiated duodenal epithelial cells and used as a model for studying the molecular mechanisms of intestine-specific transcription. has been cloned, two major DNase-I-hypersensitive sites duodenum have described, vitamin-D-response element identified. We now analysed transcription factors regulatory sequences involved CaBP9k intestine ex vivo vitro experiments. Transfection experiments intestinal (CaCo-2) non-intestinal (HeLa) cell...
N-cadherin plays a key role in axonal outgrowth and synaptogenesis, but how neurons initiate remodel N-cadherin-based adhesions remains unclear. We addressed this issue with semiartificial system consisting of coated microspheres adhering to cultured transfected for N-cadherin-GFP. Using optical tweezers, we show that growth cones are particularly reactive microspheres, which they capture few seconds drag rearward. Such strong coupling requires an intact connection between receptors...
Background: Realist synthesis is a comprehensive method of review for evaluating complex programs such as integrated models care. The first step the to clarify scope and explore existing theories in literature related targeted programs. aim elicit an initial program theory form context (C), mechanism (M) outcome (O) configurations, which serve reference model data collection analysis from studies included synthesis. Objective: To develop about scale-up care Study Design: This our realist was...
Cancers only develop if they escape immunosurveillance, and the success of cancer immunotherapies relies in most cases on their ability to restore effector T-cell functions, particularly IFNγ production. Revolutionizing treatment many cancers, targeting immune checkpoints such as PD1 can increase survival cure patients. Unfortunately, although immunotherapy has greatly improved prognosis patients, not all respond anti-PD1 immunotherapy, making it crucial identify alternative treatments that...