A5029606863- Angiogenesis and VEGF in Cancer
- Wnt/β-catenin signaling in development and cancer
- Cardiac Imaging and Diagnostics
- Cardiovascular Function and Risk Factors
- Atherosclerosis and Cardiovascular Diseases
- Coronary Interventions and Diagnostics
- Lipoproteins and Cardiovascular Health
- Peripheral Artery Disease Management
- Cell Adhesion Molecules Research
- Acute Myeloid Leukemia Research
- Kruppel-like factors research
- Acute Myocardial Infarction Research
- Congenital heart defects research
- Cardiovascular Disease and Adiposity
- Barrier Structure and Function Studies
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Adipokines, Inflammation, and Metabolic Diseases
- Cardiac Fibrosis and Remodeling
- Hedgehog Signaling Pathway Studies
- Hippo pathway signaling and YAP/TAZ
- Advanced MRI Techniques and Applications
- Tissue Engineering and Regenerative Medicine
- Neurological Disease Mechanisms and Treatments
- Cerebrovascular and Carotid Artery Diseases
- Axon Guidance and Neuronal Signaling
Inserm
2016-2025
Hôpital Cardiologique du Haut-Lévêque
2013-2025
Université de Bordeaux
2016-2025
Biologie des maladies cardiovasculaires
2016-2025
Centre Hospitalier Universitaire de Bordeaux
2016-2025
Bordeaux Population Health
2009-2024
Institute for Atherosclerosis Research
2024
Unité de recherche sur les maladies cardiovasculaires et métaboliques
2022
Lille’s Cardiology Hospital
1991-2013
Neostem (United States)
2013
Smooth muscle cells, macrophages, glial keratinocytes, and transformed cells have been established as synthesis sites for vascular endothelial growth factor (VEGF). The modulating effects of VEGF are essentially limited to (ECs), the only cell type consistently shown express receptors. has thus considered act exclusively via a paracrine pathway. We sought determine whether role human ECs might, under selected conditions, extend beyond that target involve contingency VEGF. In both...
Background —The impact of disordered lipid metabolism on collateral vessel development was studied in apolipoprotein (apo)E −/− mice with unilateral hindlimb ischemia. Methods and Results —Hindlimb blood flow capillary density were markedly reduced apoE versus C57 controls. This associated expression vascular endothelial growth factor (VEGF) the ischemic limbs mice. Cell-specific immunostaining localized VEGF protein to skeletal myocytes infiltrating T cells mice; contrast, T-cell...
We compared helical CT angiography and ventilation-perfusion radionuclide lung scanning as initial tests in the diagnosis of acute pulmonary embolism.Two hundred sixteen consecutive patients who were clinically suspected having embolism underwent angiography, scanning, Doppler sonography veins legs. On basis concordance results for on degree clinical suspicion, certain angiography. Patients without at evaluation whom no treatment was instituted followed up least 3 months to determine...
Background— FrzA/sFRP-1, a secreted, frizzled-related protein and antagonist for the wnt/frizzled pathway, is expressed in heart vessels during mouse embryogenesis adulthood. FrzA involved cell cycle control of vascular cells angiogenesis. We assessed hypothesis that could healing process after myocardial infarction (MI). Methods Results— demonstrated an upregulation sFRP-1 distinct wnt fz member expression MI. established transgenic (Tg) mice overexpress under cytomegalovirus promoter...
Mesenchymal stem cells (MSC) are multipotent postnatal cells, involved in the treatment of ischemic vascular diseases. We investigate ability MSC, exposed to short-term hypoxic conditions, participate and tissue regeneration an vivo model hindlimb ischemia. Transplantation preconditioned murine MSC (HypMSC) enhanced skeletal muscle at day 7, improved blood flow formation compared injected nonpreconditioned (NormMSC). These observed effects were correlated with increase HypMSC engraftment a...
Thrombosis is the main cause of morbidity and mortality in patients with JAK2V617F myeloproliferative neoplasms. Recent studies have reported presence endothelial cells some We investigated role that express thrombus formation using an vitro model human overexpressing vivo mice endothelial-specific expression. Interestingly, these displayed a higher propensity for thrombus. When deciphering mechanisms by which JAK2V617F-expressing promote thrombosis, we observed they pro-adhesive phenotype...
Background Because prior studies have established the critical role of endothelium in preventing vascular thrombosis and intimal thickening, we designed a series experiments to determine feasibility percutaneous local catheter delivery recombinant protein accelerate development an intact endothelial monolayer after stent implantation. Methods Results Balloon injury followed by 15-mm-long, balloon-expandable metallic was performed 64 rabbit external iliac arteries (baseline diameter,...
Vascular cell adhesion molecule-1 (VCAM-1), intercellular (ICAM-1), and E-selectin are inducible proteins involved in cell-cell adhesion. Immunohistochemical studies have indicated that human atherosclerotic plaques contain smooth muscle cells (SMCs) express ICAM-1 VCAM-1. Recently, we demonstrated SMCs culture a functionally active cytokine-inducible ICAM-1. mononuclear participate the local accumulation of cytokines related growth factors lesions. Therefore, determined effects different on...
Vascular exploration of small animals requires imaging hardware with a very high spatial resolution, capable differentiating large as well vessels, in both vivoand ex vivostudies. Micro Computed Tomography (micro-CT) has emerged recent years the preferred modality for this purpose, providing resolution 3D volumetric data suitable analysis, quantification, validation, and visualization results. The usefulness micro-CT, however, can be adversely affected by range factors including physical...
Mesenchymal stem cell (MSC) transplantation offers a great angiogenic opportunity in vascular regenerative medicine. The canonical Wnt/beta-catenin signaling pathway has been demonstrated to play an essential role fate. Recently, genetic studies have implicated the Wnt/Frizzled (Fz) molecular pathway, namely Wnt7B and Fz4, blood growth regulation. Here, we investigated whether MSC could be required shaping functional vasculature secreted Frizzled-related protein-1 (sFRP1), modulator of...
Ischaemic preconditioning (IPC) protects the heart against prolonged lethal ischaemia through a signalling cascade involving Akt, glycogen synthase kinase-3β (GSK-3β), and mitochondrial ATP-sensitive potassium channels (mitoKATP). We previously demonstrated involvement of Wnt pathway in IPC vivo via GSK-3β. A downstream target might be mammalian rapamycin (mTOR) since can impair tuberous sclerosis complex-2 (TSC2) phosphorylation by inhibiting Here, we investigate whether mTOR is involved...
Human atherosclerotic plaques contain numerous smooth muscle cells (SMCs) that express intercellular adhesion molecule-1 (ICAM-1). Expression of ICAM-1 in different is known to be regulated by tumor necrosis factor-alpha (TNF-alpha), which has recently been found present the intimal thickening human arteries. Therefore, we studied effect TNF-alpha on mRNA content and surface expression cultured aortic SMCs using methods Northern blotting immunofluorescence flow cytometry. Under basal...
Objective— The inflammatory response after myocardial infarction plays a crucial role in the healing process. Lately, there is accumulating evidence that Wnt/Frizzled pathway may play distinct inflammation. We have shown secreted frizzled-related protein-1 (sFRP-1) overexpression reduced postinfarction scar size, and we noticed decrease neutrophil infiltration ischemic tissue. aimed to further elucidate of sFRP-1 postischemic Methods Results— found vitro, was able block leukocyte activation...
A growing body of evidence supports the hypothesis that Wnt/planar cell polarity (PCP) pathway regulates endothelial proliferation and angiogenesis, but components mediate this regulation remain elusive.We investigated involvement one receptors, Frizzled4 (Fzd4), in process because its role has been implicated retinal vascular development.We found loss fzd4 function mice results a striking reduction impairment distal small artery network heart kidney. We report decreases migration ability...
Analysis of LacZ gene expression is conventionally inferred from blue staining that results exposure the transfected cells or tissue to substrate 5-bromo-4-chloro-3-indolyl-β-d-galactopyranoside (X-Gal). Such histochemical reports not whether product present absent, but where it active. We investigated hypothesis identification activity, as opposed presence, enzyme underestimates following transfer. Under conditions optimized for in vitro histochemistry, up 20% stably with nls-LacZ remained...
Objective: FrzA, a member of the group secreted frizzled related proteins (sFRP) that is expressed in cardiovascular system, has been shown to antagonize Wnt/frizzled signaling pathway. We have recently demonstrated its role vascular cell growth control vitro. In this study, we aimed examine mechanisms by which FrzA exerts antiproliferative effect on cells vitro and potential vivo. Methods results: On synchronized, growth-arrested endothelial (EC) smooth muscle (SMC) treated with recombinant...
Phosphorylation and subsequent inactivation of glycogen synthase kinase (GSK)-3beta via the Akt/PI3-Kinase pathway during ischemic preconditioning (PC) has been shown to be cardioprotective. As FrzA/sFRP-1, a secreted antagonist Wnt/Frizzled pathway, is expressed in heart able decrease phosphorylation GSK-3beta vitro on vascular cells, we examined its effect PC using transgenic mouse overexpressing FrzA cardiomyocytes (alpha-MHC promoter) under conditional transgene expression approach...