A5102813382- Angiogenesis and VEGF in Cancer
- Protein Tyrosine Phosphatases
- Cancer, Hypoxia, and Metabolism
- Cell Adhesion Molecules Research
- Fibroblast Growth Factor Research
- Retinal Diseases and Treatments
- Atherosclerosis and Cardiovascular Diseases
- Congenital heart defects research
- Glaucoma and retinal disorders
- Nitric Oxide and Endothelin Effects
- Coronary Interventions and Diagnostics
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Lymphatic System and Diseases
- Virus-based gene therapy research
- Retinal Imaging and Analysis
- Zebrafish Biomedical Research Applications
- Galectins and Cancer Biology
- Lipid metabolism and disorders
- Neonatal Respiratory Health Research
- Electrospun Nanofibers in Biomedical Applications
- Epigenetics and DNA Methylation
- Proteins in Food Systems
- Blood Coagulation and Thrombosis Mechanisms
- CRISPR and Genetic Engineering
- Proteoglycans and glycosaminoglycans research
Io Therapeutics (United States)
2014-2023
Aerpio Pharmaceuticals (United States)
2018-2022
NHS England
2020
University College Hospital
2020
Birkbeck, University of London
2020
University College London
2020
University of Antwerp
2009
Procter & Gamble (United States)
2001-2008
University of London
2005
Myriad Genetics
2004
Vascular endothelial growth factor (VEGF), also known as vascular permeability factor, induces proliferation in vitro and vivo. The human transmembrane c-fms-like tyrosine kinase Flt-1 has recently been identified a VEGF receptor. seven immunoglobulin-like extracellular domains insert sequence, features shared by two other gene-encoded proteins, domain-containing receptor (KDR) FLT-4. In this study we show that the mouse homologue of KDR, Flk-1, is second functional Flk-1 binds with high...
Recent studies have shown that application of basic fibroblast growth factor (basic FGF) to a wound has beneficial effect. However, it not been assessed whether endogenous FGF also plays role in tissue repair. In this study we found 160-fold induction mRNA encoding keratinocyte (KGF) 1 day after skin injury. This large was unique within the family FGFs, since levels acidic FGF, and FGF-5 were only slightly induced (2- 10-fold) during healing, there no expression FGF-3, FGF-4, FGF-6 detected...
ABSTRACT Fibroblast growth factors (FGFs) can influence the and differentiation of cultured cells derived from neuroectoderm, ectoderm or mesenchyme. The FGFs interact with a family at least four closely related receptor tyrosine kinases that are products individual genes. To investigate role in embryonic tissues to determine whether FGF genes might have specific functions, we compared localization mRNA for two genes, FGFR1 (the fig gene product) FGFR2 bek product), during limb formation...
Vascular endothelial growth factor (VEGF) is a polypeptide mitogen that stimulates the of cells in vitro and promotes blood vessels vivo. We have recently shown fms-like receptor tyrosine kinase (flt) for VEGF. Here we used situ hybridization to show that, adult mouse tissues, pattern flt expression was consistent with localization endothelium. also expressed endothelium during neovascularization healing skin wounds early vascular development embryos. Moreover, populations embryonic from...
Abstract Angiogenesis, the process of new vessels sprouting from existing vasculature, is a critical during early development. However, angiogenesis rarely occurs in adult, except response to cyclic hormonal stimulation ovary and uterus, injury, pathological conditions such as tumorigenesis diabetes mellitus. Tie2 (also known Tek) novel endothelium-specific receptor tyrosine kinase, which has been demonstrated be essential for development embryonic vasculature; knockout mice die by day 10.5...
Vascular endothelial (VE)–protein tyrosine phosphatase (PTP) associates with VE-cadherin, thereby supporting its adhesive activity and junction integrity. VE-PTP also Tie-2, dampening the kinase of this receptor that can support stabilization junctions. Here, we have analyzed how interference affects stability junctions in vivo. Blocking by antibodies, a specific pharmacological inhibitor (AKB-9778), gene ablation counteracted vascular leak induction inflammatory mediators. In addition,...
Retinal and choroidal neovascularization (NV) vascular leakage contribute to visual impairment in several common ocular diseases. The angiopoietin/TIE2 (ANG/TIE2) pathway maintains integrity, negative regulators of this are potential therapeutic targets for these Here, we demonstrated that endothelial-protein tyrosine phosphatase (VE-PTP), which negatively regulates TIE2 activation, is upregulated hypoxic endothelial cells, particularly retinal NV. Intraocular injection an anti-VE-PTP...
Angiogenesis is required for tumor growth and metastasis, inhibition of angiogenesis a promising approach anticancer therapy. Tie2 (a.k.a Tek) an endothelium-specific receptor tyrosine kinase known to play role in angiogenesis. To explore the therapeutic potential blocking pathway, adenoviral vector was constructed deliver recombinant, soluble (AdExTek) capable activation. Two days after i.v. injection AdExTek, plasma concentration ExTek exceeded 1 mg/ml maintained about 8 days....
We have cloned a genomic region of the murine fibroblast growth factor (FGF) receptor 1 (FGFR1) gene that includes three alternative exons for third immunoglobulinlike domain in extracellular receptor. The mRNA one these splice variants encodes secreted lacks transmembrane and cytoplasmic sequences as well portion domain. Highest levels encoding this variant were found brain, skeletal muscle, skin. expressed form FGFR1 CHO cells showed recombinant protein binds acidic FGF. also discovered...
ABSTRACT The zebrafish cloche mutation affects both the endothelial and hematopoietic lineages at a very early stage (Stainier, D. Y. R., Weinstein, B. M., Detrich, H. W., Zon, L. I. Fishman, M. C. (1995). Development 121, 3141-3150). most striking vascular phenotype is absence of endocardial cells from heart. Microscopic examination mutant embryos reveals presence endothelial-like in lower trunk tail regions while head vessels appear to be missing, indicating molecular diversification...
Background —The impact of disordered lipid metabolism on collateral vessel development was studied in apolipoprotein (apo)E −/− mice with unilateral hindlimb ischemia. Methods and Results —Hindlimb blood flow capillary density were markedly reduced apoE versus C57 controls. This associated expression vascular endothelial growth factor (VEGF) the ischemic limbs mice. Cell-specific immunostaining localized VEGF protein to skeletal myocytes infiltrating T cells mice; contrast, T-cell...
Tie2 is an endothelium-specific receptor tyrosine kinase that required for both normal embryonic vascular development and tumor angiogenesis thought to play a role in maintenance. However, the signaling pathways responsible function of remain unknown. In this report, we demonstrate p85 subunit phosphatidylinositol 3-kinase (PI3-kinase) associates with association confers functional lipid activity. Mutation 1101 abrogated vitro vivo yeast. was found activate PI3-kinase as demonstrated by...
Vascular endothelial growth factor (VEGF) was expressed in developing respiratory epithelial cells under control of the promoter from human surfactant protein C (SP-C) gene. SP-C-VEGF transgenic mice did not survive after birth. When obtained by hysterectomy on embryonic day 15 (E15) or 17 (E17), abnormalities were confined to lung and correlated with expression transgene mRNA as revealed situ hybridization. On E15 E17, marked morphogenesis observed mice. Lungs consisted large dilated...
Significance Reducing vascular leakage and stabilizing the endothelium through activation of angiopoietin (ANGPT)–TIE2 receptor tyrosine kinase pathway is a promising therapeutic strategy for diseases. ANGPT2 one two major ligands TIE2 receptor. Uniquely, possesses an agonistic role in lymphatic endothelium, but acts as competitive antagonist blood endothelium. The molecular basis opposing actions these beds poorly understood. Here we demonstrate that absence VEPTP expression confers agonist...