A5089607044- Wnt/β-catenin signaling in development and cancer
- Tissue Engineering and Regenerative Medicine
- Cell Adhesion Molecules Research
- Linguistics and Discourse Analysis
- Cancer-related gene regulation
- Kruppel-like factors research
- French Language Learning Methods
- Malaria Research and Control
- Mosquito-borne diseases and control
- Monoclonal and Polyclonal Antibodies Research
- Historical Linguistics and Language Studies
- SARS-CoV-2 and COVID-19 Research
- Protease and Inhibitor Mechanisms
- Pluripotent Stem Cells Research
- Mesenchymal stem cell research
- Viral gastroenteritis research and epidemiology
- Atherosclerosis and Cardiovascular Diseases
- Invertebrate Immune Response Mechanisms
- Connective tissue disorders research
- Cellular Mechanics and Interactions
- Research on Leishmaniasis Studies
- Cardiac Fibrosis and Remodeling
- Animal Virus Infections Studies
- Force Microscopy Techniques and Applications
- Congenital heart defects research
Sanofi (France)
1999-2025
Heidelberg University
2016-2024
Les Afriques dans le Monde
2015-2024
Université Bordeaux Montaigne
2022
Inserm
2005-2019
Université de Lille
2015-2019
Centre Hospitalier Universitaire de Lille
2019
Lille’s Cardiology Hospital
2019
University Hospital Heidelberg
2016-2017
Institut National de Recherches Archéologiques Préventives
2007-2015
Background— FrzA/sFRP-1, a secreted, frizzled-related protein and antagonist for the wnt/frizzled pathway, is expressed in heart vessels during mouse embryogenesis adulthood. FrzA involved cell cycle control of vascular cells angiogenesis. We assessed hypothesis that could healing process after myocardial infarction (MI). Methods Results— demonstrated an upregulation sFRP-1 distinct wnt fz member expression MI. established transgenic (Tg) mice overexpress under cytomegalovirus promoter...
<b>Objectives</b> To analyse the association between body mass index (BMI) and sexual activity, satisfaction, unintended pregnancies, abortions in obese people to discuss implications for public health practices, taking into account respondents’ their partners’ BMI. <b>Design</b> Random probability survey of behaviours. <b>Setting</b> National population based 12 364 men women aged 18-69 living France 2006. <b>Participants</b> selection 5535 4635 men, whom 3651 2725 were normal weight (BMI...
Mesenchymal stem cells (MSC) are multipotent postnatal cells, involved in the treatment of ischemic vascular diseases. We investigate ability MSC, exposed to short-term hypoxic conditions, participate and tissue regeneration an vivo model hindlimb ischemia. Transplantation preconditioned murine MSC (HypMSC) enhanced skeletal muscle at day 7, improved blood flow formation compared injected nonpreconditioned (NormMSC). These observed effects were correlated with increase HypMSC engraftment a...
We evaluated our SARS-CoV-2 prefusion spike recombinant protein vaccine (CoV2 preS dTM) with different adjuvants, unadjuvanted, and in a one-injection two-injection dosing schedule previous phase 1-2 study. Based on interim results from that study, we selected the AS03 adjuvant for further clinical development. However, lower than expected antibody responses, particularly older adults, higher reactogenicity after second vaccination were observed. In current safety immunogenicity of an...
Vascular cell adhesion molecule-1 (VCAM-1), intercellular (ICAM-1), and E-selectin are inducible proteins involved in cell-cell adhesion. Immunohistochemical studies have indicated that human atherosclerotic plaques contain smooth muscle cells (SMCs) express ICAM-1 VCAM-1. Recently, we demonstrated SMCs culture a functionally active cytokine-inducible ICAM-1. mononuclear participate the local accumulation of cytokines related growth factors lesions. Therefore, determined effects different on...
Mesenchymal stem cell (MSC) transplantation offers a great angiogenic opportunity in vascular regenerative medicine. The canonical Wnt/beta-catenin signaling pathway has been demonstrated to play an essential role fate. Recently, genetic studies have implicated the Wnt/Frizzled (Fz) molecular pathway, namely Wnt7B and Fz4, blood growth regulation. Here, we investigated whether MSC could be required shaping functional vasculature secreted Frizzled-related protein-1 (sFRP1), modulator of...
Profilin is an actin monomer binding protein that provides ATP-actin for incorporation into filaments. In contrast to higher eukaryotic cells with their large filamentous structures, apicomplexan parasites typically contain only short and highly dynamic microfilaments. apicomplexans, profilin appears be the main monomer-sequestering protein. Compared classical profilins, profilins additional arm-like β-hairpin motif, which we show here critically involved in binding. Through comparative...
Human atherosclerotic plaques contain numerous smooth muscle cells (SMCs) that express intercellular adhesion molecule-1 (ICAM-1). Expression of ICAM-1 in different is known to be regulated by tumor necrosis factor-alpha (TNF-alpha), which has recently been found present the intimal thickening human arteries. Therefore, we studied effect TNF-alpha on mRNA content and surface expression cultured aortic SMCs using methods Northern blotting immunofluorescence flow cytometry. Under basal...
Objective— The inflammatory response after myocardial infarction plays a crucial role in the healing process. Lately, there is accumulating evidence that Wnt/Frizzled pathway may play distinct inflammation. We have shown secreted frizzled-related protein-1 (sFRP-1) overexpression reduced postinfarction scar size, and we noticed decrease neutrophil infiltration ischemic tissue. aimed to further elucidate of sFRP-1 postischemic Methods Results— found vitro, was able block leukocyte activation...
A growing body of evidence supports the hypothesis that Wnt/planar cell polarity (PCP) pathway regulates endothelial proliferation and angiogenesis, but components mediate this regulation remain elusive.We investigated involvement one receptors, Frizzled4 (Fzd4), in process because its role has been implicated retinal vascular development.We found loss fzd4 function mice results a striking reduction impairment distal small artery network heart kidney. We report decreases migration ability...
Migration of malaria parasites is powered by a myosin motor that moves actin filaments, which in turn link to adhesive proteins spanning the plasma membrane. The retrograde flow these adhesins appears be coupled forward locomotion. However, contact dynamics between parasite and substrate as well generation forces are complex their relation unclear. Using optical tweezers we found rates up 15 μm/s contrasting with average speeds 1–2 μm/s. We surface protein, TLP, functions reducing for...
Objective: FrzA, a member of the group secreted frizzled related proteins (sFRP) that is expressed in cardiovascular system, has been shown to antagonize Wnt/frizzled signaling pathway. We have recently demonstrated its role vascular cell growth control vitro. In this study, we aimed examine mechanisms by which FrzA exerts antiproliferative effect on cells vitro and potential vivo. Methods results: On synchronized, growth-arrested endothelial (EC) smooth muscle (SMC) treated with recombinant...
Abstract —Vitronectin (VN) is a plasma glycoprotein that promotes cell attachment and induces migration of human smooth muscle cells (SMCs) in culture. VN has been observed to accumulate atherosclerotic plaques, although its origin role atherosclerosis are not yet established. In the present experiments, synthesis by intimal colocalization with receptors, αvβ3 αvβ5, were studied situ hybridization immunohistochemistry on 15 plaques from carotid arteries obtained after surgery. Strong protein...
Phosphorylation and subsequent inactivation of glycogen synthase kinase (GSK)-3beta via the Akt/PI3-Kinase pathway during ischemic preconditioning (PC) has been shown to be cardioprotective. As FrzA/sFRP-1, a secreted antagonist Wnt/Frizzled pathway, is expressed in heart able decrease phosphorylation GSK-3beta vitro on vascular cells, we examined its effect PC using transgenic mouse overexpressing FrzA cardiomyocytes (alpha-MHC promoter) under conditional transgene expression approach...
Angiogenesis involves the coordinated growth and migration of endothelial cells (ECs) toward a proangiogenic signal. The Wnt planar cell polarity (PCP) pathway, through recruitment Dishevelled (Dvl) Dvl-associated activator morphogenesis (Daam1), has been proposed to regulate actin cytoskeleton microtubule (MT) reorganization for oriented migration. Here we report that Kif26b--a kinesin--and Daam1 cooperatively initiation EC sprouting directional via MT reorganization. First, find Kif26b is...
Vascular cell adhesion molecule-1 (VCAM-1) and its counterreceptor, the integrin very late antigen-4 (VLA-4), have recently been identified in smooth muscle cells during intimal thickening humans newly forming vessels ontogeny mice, respectively. We examined coexpression of VCAM-1 alpha 4 subunit human cells. The expression were studied development aorta. In 10-week-old fetal aorta, strongly expressed Their was dramatically reduced within 24th week gestation disappeared adult aortic media....
Gliding motility allows malaria parasites to migrate and invade tissues cells in different hosts. It requires parasite surface proteins provide attachment host extracellular matrices. Here, we identify the Plasmodium protein LIMP (the name refers a gliding phenotype sporozoite arising from epitope tagging of endogenous protein) as key regulator for adhesion during rodent model P. berghei. Transcribed gametocytes, is translated ookinete maternal mRNA, later sporozoite. The absence reduces...
Vascular permeability is essential for the health of normal tissues and an important characteristic many disease states. The role Wnt/frizzled pathway in vascular biology has recently been reported. objectives this study are to analyse Frizzled7 (Fzd7) receptor control integrity. Fzd7 expressed endothelial cells accumulates at points cell–cell contact association with VE-cadherin β-catenin, two major adherens junction molecules. To selectively delete fzd7 vasculature, we developed gene...
Respiratory syncytial virus (RSV) is a leading cause of lower respiratory tract infection and hospitalisations in infants worldwide. The primary analyses HARMONIE showed that nirsevimab reduced infant due to RSV-associated through the RSV season. This analysis aims evaluate nirsevimab's efficacy at 180 days after dosing, period exceeding typical 5-month an ongoing, open-label, parallel arm, randomised, controlled, phase 3b study conducted France, Germany, UK. Infants aged 12 months or...
Abstract Undifferentiated cells have been identified in the prenatal blastocyst, inner cell mass, and gonadal ridges of rodents primates, including humans. After isolation these express molecular immunological markers for embryonic cells, capabilities extended self‐renewal, telomerase activity. When allowed to differentiate, stem phenotypic tissues ectodermal, mesodermal, endodermal origin. implanted vivo, undifferentiated noninduced formed teratomas. In this report we describe a clone...