A5089330166- Intensive Care Unit Cognitive Disorders
- RNA and protein synthesis mechanisms
- RNA Research and Splicing
- Anesthesia and Sedative Agents
- Anesthesia and Neurotoxicity Research
- RNA modifications and cancer
- Lipid metabolism and disorders
- Advanced Electron Microscopy Techniques and Applications
- Traumatic Brain Injury and Neurovascular Disturbances
- Advancements in Semiconductor Devices and Circuit Design
- S100 Proteins and Annexins
- Molecular Biology Techniques and Applications
- interferon and immune responses
- Viral Infections and Outbreaks Research
- Adipokines, Inflammation, and Metabolic Diseases
- Blood properties and coagulation
- Semiconductor materials and devices
- SARS-CoV-2 and COVID-19 Research
Oncode Institute
2024
Radboud University Nijmegen
2021-2024
Radboud Institute for Molecular Life Sciences
2021
Radboud University Medical Center
2021
Universidade do Extremo Sul Catarinense
2010-2013
Delirium is a frequent and serious problem in the intensive care unit (ICU) that associated with increased mortality, prolonged mechanical ventilation, hospital length of stay (LOS). The main objective present study was to compare assess agreement between diagnosis delirium obtained by Confusion Assessment Method for ICU (CAM-ICU) Intensive Care Screening Checklist (ICDSC) patients admitted their association outcomes.Adult more than 24 hours May November 2008 were included. Patients Richmond...
The aim of this study was to determine the association between serum concentrations brain-derived neurotrophic factor (BDNF), neuron-specific enolase (NSE), and S100β occurrence delirium in critically ill patients.This case-control included 30 patients with matched controls a 16-bed general intensive care unit (ICU). Serum BDNF, NSE, S100 were determined by enzyme-linked immunosorbent assay assays at time ICU admission on day before diagnosed. Delirium diagnosed confusion assessment method...
SUMMARY Poly(A)-tails are crucial for mRNA translation and degradation, but the exact relationship between tail length kinetics remains unclear. Here we employ a small library of identical mRNAs that differ only in their poly(A)-tail to examine behavior human embryonic kidney cells. We find strongly correlates with degradation rates, is decoupled from translation. Interestingly, an optimal ∼100 nucleotides displays highest rate, which average endogenous measured by nanopore sequencing....
Objective: To evaluate the relationship between brain damage biomarkers and mortality in intensive care unit (ICU). Methods: The sample comprised 70 patients admitted to an ICU. Blood samples were collected from all on ICU admission, levels of S100β neuron-specific enolase (NSE) determined by ELISA. Results: Acute Physiologic Chronic Health Evaluation (APACHE II) score was associated with mortality, but NSE not this outcome. In contrast, significantly higher delirious non-delirious who...
Quantifying the number of proteins that interact with mRNAs, in particular poly(A) tails is crucial for understanding gene regulation. Biochemical assays offer significant advantages this purpose. Here, we present a protocol synthesizing mRNAs accurate, length-specific through PCR-based approach. We also describe steps an vitro (i.e., cell-free) approach visualizing sequential binding Cytoplasmic Poly(A)-Binding Proteins (PABPCs) to these tails. detail quality control throughout procedure....
Long non-coding RNAs are nucleotide molecules that regulate transcription in numerous cellular processes and related to the occurrence of many diseases, including cancer. In this regard, we recently discovered a polyadenylated long RNA (named TG2-lncRNA) encoded within first intron Transglutaminase type 2 gene (TGM2), which is tumour proliferation human cancer cell lines. To better characterize new biological player, investigated effects its suppression MCF-7 breast cells, using siRNA...